A polysaccharide from Sargassum thunbergii inhibits angiogenesis via downregulating MMP-2 activity and VEGF/HIF-1α signaling

Ou, Minghui; Sun, Xianqiang; Liang, Jian; Liu, Fei; Wang, Liqin; Wu, Xiaomin; Tu, Jie

doi:10.1016/j.ijbiomac.2016.10.046

Cited by 27 publications

(21 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the previous study [8], the expressions of VEGF-A and HIF-1α in the endothelial cells were significantly downregulated by the STPC2 treatment. However, the molecular mechanism of anti-angiogenesis mediated by STPC2 has not been demonstrated very well, thus we verified whether the modulation of VEGF-A and HIF-1α was related to the inhibition of VEGFR2 phosphorylation and its subsequent downstream signaling.…”

Section: Resultsmentioning

confidence: 95%

“…It was found that STPC2 was composed of a high ratio of fucose. In addition, its sulfate content was also relatively high, up to 27.8% [8]. In previous studies and reviews, the sulfation was reported to be associated with the contribution of anti-angiogenesis activity [7,9].…”

Section: Resultsmentioning

confidence: 99%

“…As elucidated in the previous study [8], STPC2 was demonstrated to be a potential anti-angiogenic agent, but the determinants were still unknown. As discussed in the review [7], the angiogenesis activity of the fucoidans was not only significantly determined by the degree of sulfation, but also highly dependent on their molecular weight, namely fucoidans of high molecular weight were tightly connected with the anti-angiogenic activity, whereas fucoidans of low molecular weight might correlate to their pro-angiogenic capability.…”

Section: Resultsmentioning

confidence: 99%

“…In the previous study [8], STPC2 could downregulate the expression of hypoxia-inducible-factor-1α (HIF-1α) and VEGF, which might impede vascular permeability. However, the molecular mechanism of STPC2 regulating the VEGF expression to affect the anti-angiogenic activity has been illustrated well.…”

Section: Discussionmentioning

confidence: 99%

“…The native polysaccharide, STPC2, was prepared and characterized in the previous publication [8]. Briefly, all the brown alga was extracted from the boiling-water and subjected to precipitation with ethanol.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Structural Determinant and Its Underlying Molecular Mechanism of STPC2 Related to Anti-Angiogenic Activity

Cui

et al. 2017

Marine Drugs

View full text Add to dashboard Cite

In this study, we aimed to use different strategies to further uncover the anti-angiogenic molecular mechanism of a fucoidan-like polysaccharide STPC2, isolated from brown alga Sargassum thunbergii. A desulfated derivative, STPC2-DeS, was successfully prepared and identified. The native polysaccharide and desulfated product were subjected to evaluate their anti-angiogenic effects. In the tube formation assay, STPC2 showed dose-dependent inhibition. In addition, STPC2 could distinctly inhibit the permeation of HUVEC cells into the lower chamber. Moreover, a significant reduction of microvessel density was observed in chick chorioallantoic membrane assay treated with STPC2. Meanwhile, STPC2 was found to repress the VEGF-induced neovessel formation in the matrigel plug assay in vivo. However, STPC2-DeS failed to suppress the anti-angiogenic activity via these in vitro and in vivo strategies. In addition, we demonstrated that STPC2 could significantly downregulate the phosphorylation of VEGFR2 and its related downstream Src family kinase, focal adhesion kinase, and AKT kinase. Furthermore, surface plasmon resonance assay revealed that STPC2 bound strongly to VEGF to interfere with VEGF–VEGFR2 interaction. Taken together, these results evidently demonstrated that STPC2 exhibited a potent anti-angiogenic activity through binding to VEGF via sulfated groups to impede VEGF–VEGFR2 interaction, thus affected the downstream signaling molecules.

show abstract

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Structural Determinant and Its Underlying Molecular Mechanism of STPC2 Related to Anti-Angiogenic Activity

Cui

et al. 2017

Marine Drugs

View full text Add to dashboard Cite

show abstract

Marine Algal Phlorotannins and their Biological Importance

Thomas

Diyya

Ghafour

et al. 2020

Encyclopedia of Marine Biotechnology

View full text Add to dashboard Cite

Sema3A and HIF1α co‐overexpressed iPSC‐MSCs/HA scaffold facilitates the repair of calvarial defect in a mouse model

Wang

et al. 2020

Journal Cellular Physiology

View full text Add to dashboard Cite

Mesenchymal stem/stromal cells (MSCs) play an important role in bone tissue engineering because MSCs possess multilineage potential of differentiation to mesenchymal tissues. Semaphorin 3A (Sema3A) and hypoxia-inducible factor-1α (HIF1α) are proved as important regulatory factors for osteogenesis and angiogenesis.The aim of this study was to investigate the effects of Sema3A and HIF1α co-overexpression on the osteogenesis and angiogenesis in induced pluripotent stem cell-derived mesenchymal stem cells (iPSC-MSCs). Importantly, we assessed the potential osteogenic effectiveness of Sema3A and HIF1α co-overexpressed iPSC-MSCs seeded on hydroxyapatite (HA) scaffold in a mouse calvarial defect model. The overexpression for Sema3A, HIF1α, or Sema3A-HIF1α fusion in iPSC-MSCs was performed by separately infecting with conducted lentiviral vector. We determined the

show abstract

A polysaccharide from Sargassum thunbergii inhibits angiogenesis via downregulating MMP-2 activity and VEGF/HIF-1α signaling

Cited by 27 publications

References 30 publications

Structural Determinant and Its Underlying Molecular Mechanism of STPC2 Related to Anti-Angiogenic Activity

Structural Determinant and Its Underlying Molecular Mechanism of STPC2 Related to Anti-Angiogenic Activity

Marine Algal Phlorotannins and their Biological Importance

Sema3A and HIF1α co‐overexpressed iPSC‐MSCs/HA scaffold facilitates the repair of calvarial defect in a mouse model

Contact Info

Product

Resources

About