A porcine epidemic diarrhea virus strain with distinct characteristics of four amino acid insertion in the COE region of spike protein

Ji, Zhaoyang; Da, Shi; Shi, Hongyan; Wang, Xiaobo; Chen, Jianfei; Li, Jianbo; Ye, Dandan; Jing, Zhaoyang; Liu, Qiuge; Fan, Qianjin; Li, Mingwei; Cong, Guangyi; zhang, Jiyu; Han, Yuru; Zhang, Xin; Feng, Li

doi:10.1016/j.vetmic.2020.108955

Cited by 12 publications

(15 citation statements)

References 37 publications

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“…The antigenic epitopes inducing neutralizing antibodies against PEDV infection in pigs are mainly located in the S glycoprotein. The mutations therefore more frequently occur in the S protein of PEDV [ 40 , 41 , 42 ]. Mutations in the S protein can lead to a variety of structural and functional consequences and contribute to the lack of efficacy of PEDV vaccines [ 42 , 43 , 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…The mutations therefore more frequently occur in the S protein of PEDV [ 40 , 41 , 42 ]. Mutations in the S protein can lead to a variety of structural and functional consequences and contribute to the lack of efficacy of PEDV vaccines [ 42 , 43 , 44 , 45 ]. In this study, the two particular substitutions, N537S and Y561H, within the COE domain of the S protein could be significant in the pathogenetic nature of the PEDV strain isolated from the SL farm.…”

Section: Discussionmentioning

confidence: 99%

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Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback)

Thi

Chen

Chung

et al. 2022

Viruses

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The feedback strategy, or controlled exposure of pig herd to the porcine epidemic diarrhea virus (PEDV), significantly decreased losses during a severe outbreak in late 2013 in Taiwan. However, some pig farms still suffered from recurrent outbreaks. To evaluate the association between antibody titers and clinical manifestations, sera and colostra were analyzed from one pig farm that employed the feedback strategy. Furthermore, spike (S) gene full sequences from six positive samples of two farms with and without using feedback were compared to investigate the evolution of PEDV variants circulating in pig herds. The results in this study showed that high PEDV antibody titers do not correlate with the high rate of protection from PEDV infection. In addition, repeated feedback generated the emergence of PEDV variants with unique substitutions of N537S and Y561H in the COE domain and S769F in the SS6 epitopes. These mutations indicated the pathogenetic evolution of PEDV strains existing in the cycle of the feedback method. A very strict biosecurity practice to block the routes of pathogen transfer should be followed to achieve successful control of PEDV infections in pig herds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback)

Thi

Chen

Chung

et al. 2022

Viruses

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“…The S1 region is responsible for viral-host recognition and receptor binding, whereas the S2 region is responsible for membrane fusion and internalization. Mutations occurring in the S gene can result in amino acid changes that affect pathogenicity, transmissibility, antigenic properties, and reactivity with neutralizing antibodies [ 20 ]. The S1 region functions as a receptor-binding domain (RBD) and has the ability to recognize a variety of host receptors, including proteins and sugars, that can trigger humoral and cellular responses.…”

mentioning

confidence: 99%

“…The S1 region functions as a receptor-binding domain (RBD) and has the ability to recognize a variety of host receptors, including proteins and sugars, that can trigger humoral and cellular responses. Meanwhile, the S2 subunit mediates the membrane fusion process, leading to the release of viral RNA into the host cell [ 11 , 20 ]. S1 contains two main domains: the N-terminal domain (S1-NTD) and the C-terminal domain (S1-CTD) [ 17 ].…”

mentioning

confidence: 99%

“…As the S protein contains various immunodominant epitopes, this high level of mutation may affect vaccine efficacy. Vaccines derived from classical strains in the G1 group are highly effective against classical PEDV strains but do not provide adequate protection against highly virulent strains in the G2 group [ 20 ]. The S1 subunit has an even higher mutation rate than the full S gene, with 1.5 × 10 −3 substitutions/site/year [ 24 ].…”

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confidence: 99%

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Molecular analysis reveals a distinct subgenogroup of porcine epidemic diarrhea virus in northern Vietnam in 2018–2019

et al. 2022

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The spike protein (S) of porcine epidemic diarrhea virus (PEDV), in particular, the C-terminal domain of the S1 subunit (S1-CTD), which contains the conserved CO26K-equivalent (COE) region (aa 499–638), which is recognized by neutralizing antibodies, exhibits a high degree of genetic and antigenic diversity. We analyzed 61 PEDV S1-CTD sequences (630 nt), including 26 from samples collected from seven provinces in northern Vietnam from 2018 to 2019 and 35 other sequences, representing the G1a and 1b, G2a and 2b, and recombinant (G1c) genotypes and vaccines. The majority (73.1%) of the strains (19/26) belonged to subgroup G2b. In a phylogenetic analysis, seven strains were clustered into an independent, distinct subgenogroup named dsG with strong nodal support (98%), separate from both G1a and G1b as well as G2a, 2b, and G1c. Sequence analysis revealed distinct changes (513 T>S , 520 G>D , 527 V>(L/M) , 591 L>F , 669 A>(S/P) , and 691 V>I ) in the COE and S1 D regions that were only identified in these Vietnamese strains. This cluster is a new antigenic variant subgroup, and further studies are required to investigate the antigenicity of these variants. The results of this study demonstrated the continuous evolution in the S1 region of Vietnamese PEDV strains, which emphasizes the need for frequent updates of vaccines for effective protection. Supplementary Information The online version contains supplementary material available at 10.1007/s00705-022-05580-x.

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Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Xinjiang, China, from 2020 to 2022

Chen,

Tian,

Liu

et al. 2024

Arch Virol

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A porcine epidemic diarrhea virus strain with distinct characteristics of four amino acid insertion in the COE region of spike protein

Cited by 12 publications

References 37 publications

Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback)

Antibody Evaluation and Mutations of Antigenic Epitopes in the Spike Protein of the Porcine Epidemic Diarrhea Virus from Pig Farms with Repeated Intentional Exposure (Feedback)

Molecular analysis reveals a distinct subgenogroup of porcine epidemic diarrhea virus in northern Vietnam in 2018–2019

Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Xinjiang, China, from 2020 to 2022

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