A portable and low-cost centrifugal microfluidic platform for multiplexed colorimetric detection of protein biomarkers

Akhtar, Ahmad S.; Pinto, Inês F.; Soares, Ruben R. G.; Russom, Aman

doi:10.1016/j.aca.2023.340823

Cited by 14 publications

(10 citation statements)

References 53 publications

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“…This high-throughput assay can effectively reduce detection costs and upgrade the efficiency of resource utilization. 64,65 ■ SIGNAL TRANSDUCTION MECHANISMS FOR MULTIPLEXED PEC SENSORS Enzyme-Catalyzed Regulation Strategy. The primary challenge in applying traditional PEC sensors to multiplex detection is the high likelihood of cross-interference of multiplex detection signals at a single recognition interface.…”

Section: General Detection Principlesmentioning

confidence: 99%

“…However, the portability of the sensor is considered a requirement for practical applications. 65,89 Despite the existence of studies on the construction of miniature PEC sensors using microfluidic chips, achieving practical miniaturization remains challenging owing to the presence of electrochemical workstations and excitation light sources. In terms of the miniaturization and integration design of PEC sensor devices, the miniaturization of the identification and sensing unit and the integration of hand-held instruments and miniaturized signaling devices, such as smartphones, 15,90,91 multimeters, 92−94 pH meters, 95 and thermometers, 7,96,97 with PEC sensing technology should be considered.…”

Section: General Detection Principlesmentioning

confidence: 99%

“…Multiplex PEC detection technology can be used to obtain data from multiplex indicators in a single test. This high-throughput assay can effectively reduce detection costs and upgrade the efficiency of resource utilization. , …”

Section: General Detection Principles and Technical Advantages For Mu...mentioning

confidence: 99%

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Multiplex Signal Transduction and Output at Single Recognition Interface of Multiplexed Photoelectrochemical Sensors

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Chen,

et al. 2024

Anal. Chem.

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Section: General Detection Principlesmentioning

confidence: 99%

Section: General Detection Principlesmentioning

confidence: 99%

See 1 more Smart Citation

Multiplex Signal Transduction and Output at Single Recognition Interface of Multiplexed Photoelectrochemical Sensors

Li,

Chen,

et al. 2024

Anal. Chem.

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“…Conventional manufacturing techniques used for microfluidic devices require a clean room and generate high costs, which hinder their widespread adoption ( Walsh et al, 2017 ; Shakeri et al, 2021 ). Consequently, there has been a growing interest in developing low-cost techniques to fabricate microfluidic devices, facilitating broader access and expediting integration into various applications ( Li et al, 2022 ; Akhtar et al, 2023 ; Li et al, 2023 ).…”

Section: Manufacturing Techniquesmentioning

confidence: 99%

Breaking the clean room barrier: exploring low-cost alternatives for microfluidic devices

Rodríguez

Andrade-Pérez

Vargas

et al. 2023

Front. Bioeng. Biotechnol.

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Microfluidics is an interdisciplinary field that encompasses both science and engineering, which aims to design and fabricate devices capable of manipulating extremely low volumes of fluids on a microscale level. The central objective of microfluidics is to provide high precision and accuracy while using minimal reagents and equipment. The benefits of this approach include greater control over experimental conditions, faster analysis, and improved experimental reproducibility. Microfluidic devices, also known as labs-on-a-chip (LOCs), have emerged as potential instruments for optimizing operations and decreasing costs in various of industries, including pharmaceutical, medical, food, and cosmetics. However, the high price of conventional prototypes for LOCs devices, generated in clean room facilities, has increased the demand for inexpensive alternatives. Polymers, paper, and hydrogels are some of the materials that can be utilized to create the inexpensive microfluidic devices covered in this article. In addition, we highlighted different manufacturing techniques, such as soft lithography, laser plotting, and 3D printing, that are suitable for creating LOCs. The selection of materials and fabrication techniques will depend on the specific requirements and applications of each individual LOC. This article aims to provide a comprehensive overview of the numerous alternatives for the development of low-cost LOCs to service industries such as pharmaceuticals, chemicals, food, and biomedicine.

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“…The last few decades have shown broader access of these microdevices thereby expediting their integration across diverse areas including biomedical, point of care, pharmaceutical, chemical, and health care [2][3][4][5]. Consequently, the growing demand for microfluidic devices especially in bio-analytical applications [6] facilitates the development of a low-cost technique with high throughput production potential [7][8][9]. Microdevices can be manufactured either through direct fabrication or a replication approach [10].…”

Section: Introductionmentioning

confidence: 99%

Design and fabrication of microfluidic devices: a cost-effective approach for high throughput production

Thomas,

Agrawal

2023

J. Micromech. Microeng.

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Microdevices have been recognized as a potential platform for performing numerous biomedical analysis and diagnostic applications. However, promising and viable techniques for a cost-effective and high throughput production of microfluidic devices still remain as a challenge. This paper addresses this problem with an alternative solution for the fabrication of microfluidic devices in a simple and efficient manner. We utilized laser-assisted engraving technique to fabricate a master mold on an acrylic sheet of different thicknesses from 4 to 20mm. Low cost indigenously developed CO2 (10.6μm wavelength) laser engraving device was used for the experiments. The effect of various laser parameters such as power and speed of operation on the height of engraved structures was studied in detail. Optimal engraving results were obtained with a laser speed of 200–250mm s−1 with a spacing interval of 0.002mm at a laser power of 10–12W. Master mold of microdevice with a channel width of 100μm were produced using this technique. The replica transfer was performed by a simple imprinting method using a benchtop universal testing machine that can provide a maximum compressive load upto 1kN. The replicas were successfully generated on various thin film substrates including polymers, plastics, Whatman filter paper, teflon, vinyl sheets, copper, and aluminum sheets. The effect of load applied on the depth of the microfluidic channel was studied for the substrates such as teflon and Whatman filter paper. A load of 1kN can generate a depth of a few hundred micrometers on various substrates mentioned above. The replicas were also transferred to thermoformable PETG (polyethylene terephthalate glycol) sheets under load with an elevated temperature. The channel-imprinted PETG substrates were later sandwiched between two acrylic sheets with adhesive-coated polymer sheets and screws at the corners. Soft lithographic techniques were also performed to replicate the channel on a poly dimethyl siloxane substrate which was later bonded to a glass plate using an oxygen plasma cleaner device. Fluidic flow testing was conducted by pumping dye-mixed deionized (DI) water through the channels using a syringe pump and connecting tubes at a constant flow rate of 5ml min−1. The outcomes of this study provide an alternative solution for a simple and low-cost method for microdevice fabrication at a large scale.

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A portable and low-cost centrifugal microfluidic platform for multiplexed colorimetric detection of protein biomarkers

Cited by 14 publications

References 53 publications

Multiplex Signal Transduction and Output at Single Recognition Interface of Multiplexed Photoelectrochemical Sensors

Multiplex Signal Transduction and Output at Single Recognition Interface of Multiplexed Photoelectrochemical Sensors

Breaking the clean room barrier: exploring low-cost alternatives for microfluidic devices

Design and fabrication of microfluidic devices: a cost-effective approach for high throughput production

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