A portable instrument for the optical interrogation of a novel biochip

Baldini, Francesco; Bolzoni, L.; Giannetti, Ambra; Porro, G. Bianchi; Trono, C.

doi:10.1117/12.866456

Cited by 1 publication

(2 citation statements)

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“…The working principle of the device was already described in previous papers [26,27], but it is here shortly presented again for clarity. The optical beam of a laser diode emitting at 635 nm (Hitachi HL6314MG), properly filtered with a band pass filter at 635 nm and focused by means of a cylindrical lens, illuminates from the top of the chip a single microchannel, and consequently the sensing layer.…”

Section: The Integrated Optical Detection Systemmentioning

confidence: 99%

“…The optical beam of a laser diode emitting at 635 nm (Hitachi HL6314MG), properly filtered with a band pass filter at 635 nm and focused by means of a cylindrical lens, illuminates from the top of the chip a single microchannel, and consequently the sensing layer. The emitted fluorescence, which comes from the sensing layer where the specific biologic interaction takes place, is mainly coupled to the transparent waveguides running over each microchannel; this occurs since the emitted fluorescence is anisotropic and directed towards the denser medium, i.e., the PMMA, being the fluorophore located at a distance from the medium interface smaller than the emission wavelength; the emitted fluorescence is guided within the PMMA waveguides since the anisotropic emitted fluorescence has well-defined preferential directions, characterized by angles larger than the total reflection angle [11,[27][28][29]. The guided fluorescence is laterally collected by a single plastic optical fiber connected to a cooled photodiode (Hamamatsu S9295).…”

Section: The Integrated Optical Detection Systemmentioning

confidence: 99%

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Towards an Integrated System as Point-of-Care Device for the Optical Detection of Sepsis Biomarkers

et al. 2020

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Severe infection and sepsis are a common, expensive, and frequently fatal conditions in critically ill patients. The sepsis diagnosis is not trivial, since it is an extremely complex chain of events involving inflammatory and anti-inflammatory processes, cellular reactions, and circulatory disorders. For these reasons, delay in diagnosis and initiation of drug treatments have shown to be crucial for this pathology. Moreover, a multitude of biomarkers has been proposed, many more than for other pathologies. In order to select optimal treatments for the highly heterogeneous group of sepsis patients and to reduce costs, novel multiplexed tools that better characterize the patient and his or her specific immune response are highly desired. In order to achieve the fundament of drastically improved multi-analyte detection and to attain low limits of detection in diagnostics, the area of point-of-care testing (POCT) technology is developing quickly, leading to the production of instruments, the reliability of which is continuously increasing. For this purpose, a selection of two biomarkers—C-reactive protein (CRP) and neopterin (NP)—was studied in this paper and a fluorescence-based integrated optical system, suitable for future POCT applications, was implemented that is capable of performing the simultaneous measurement of the two different biomarkers in replicate. A limit of detection of 10 and 2.1 µg L−1 was achieved for CRP and NP spiked in commercially available human serum, respectively. Moreover, measurements on both biomarkers were also performed on serum samples collected from septic patients.

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Section: The Integrated Optical Detection Systemmentioning

confidence: 99%