A portable paper-based microfluidic platform for multiplexed electrochemical detection of human immunodeficiency virus and hepatitis C virus antibodies in serum

Zhao, Changzhi; Liu, Xinyu

doi:10.1063/1.4945311

Cited by 77 publications

(53 citation statements)

References 34 publications

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“…[34][35][36][37] The strengths of PADs have been exploited as powerful analytical tools for a variety of diagnostic intents including, but not limited to the following: (1) a multiplexed electrochemical platform to detect human immunodeficiency virus (HIV) and hepatitis C virus (HCV) with limits of detection (LODs) for HIV and HCV assays of 300 pg/ml and 750 pg/ml, respectively; and (2) a three-dimensional detection sensor with a linear response for nitrite concentrations of 0-11.5 ppm and oxalate concentrations of 0-1000 mg/100 ml. 38,39 Additionally, paper-based hybrid systems have been presented in response to hard flow control challenges and assay observation that suffers from opaque material properties. 40,41 Nowadays, the development of simple pesticide biosensors mainly focuses on the quantification of organophosphorus or carbamate compounds in food or water because both pesticides are widely used in agriculture.…”

Section: Introductionmentioning

confidence: 99%

Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

et al. 2016

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This article unveils the development of a paper-based analytical device designed to rapidly detect and clinically diagnose paraquat (PQ) poisoning. Using wax printing technology, we fabricated a PQ detection device by pattering hydrophobic boundaries on paper. This PQ detection device employs a colorimetric sodium dithionite assay or an ascorbic acid assay to indicate the PQ level in a buffer system or in a human serum system in 10 min. In this test, colorimetric changes, blue in color, were observable with the naked eye. By curve fitting models of sodium dithionite and ascorbic acid assays in normal human serum, we evaluated serum PQ levels for five PQ-poisoned patients before hemoperfusion (HP) treatment and one PQpoisoned patient after HP treatment. As evidenced by similar detection outcomes, the analytical performance of our device can compete with that of the highest clinical standard, i.e., spectrophotometry, with less complicated sample preparation and with more rapid results. Accordingly, we believe that our rapid PQ detection can benefit physicians determining timely treatment strategies for PQ-poisoned patients once they are taken to hospitals, and that this approach will increase survival rates.Published by AIP Publishing. [http://dx

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Section: Introductionmentioning

confidence: 99%

Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

et al. 2016

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“…(For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.) 2E) (Channon et al, 2018;Zhao and Liu, 2016). 2D) (Speller et al, 2019) and paper-based methods ( Fig.…”

Section: Technology For Loc Fabricationmentioning

confidence: 99%

Recent advances in lab-on-a-chip technologies for viral diagnosis

Zhu

Fohlerová

Pekárek

et al. 2020

Biosensors and Bioelectronics

195

146

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A B S T R A C TThe global risk of viral disease outbreaks emphasizes the need for rapid, accurate, and sensitive detection techniques to speed up diagnostics allowing early intervention. An emerging field of microfluidics also known as the lab-on-a-chip (LOC) or micro total analysis system includes a wide range of diagnostic devices. This review briefly covers both conventional and microfluidics-based techniques for rapid viral detection. We first describe conventional detection methods such as cell culturing, immunofluorescence or enzyme-linked immunosorbent assay (ELISA), or reverse transcription polymerase chain reaction (RT-PCR). These methods often have limited speed, sensitivity, or specificity and are performed with typically bulky equipment. Here, we discuss some of the LOC technologies that can overcome these demerits, highlighting the latest advances in LOC devices for viral disease diagnosis. We also discuss the fabrication of LOC systems to produce devices for performing either individual steps or virus detection in samples with the sample to answer method. The complete system consists of sample preparation, and ELISA and RT-PCR for viral-antibody and nucleic acid detection, respectively. Finally, we formulate our opinions on these areas for the future development of LOC systems for viral diagnostics.

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“…The creation of simple and cost‐effective methods to print electrodes on μPADs has expanded their use in quantitative analysis of biomarkers. Thus, a microfluidic paper‐based electrochemical device (μPED), tested in mouse serum, was able to detect HIV p24 core antigen and HCV core antigen with limits of detection of 300 pg/mL and 750 pg/mL, respectively …”

Section: Integrationmentioning

confidence: 99%

Innovative technologies for point‐of‐care testing of viral hepatitis in low‐resource and decentralized settings

Duchesne

Lacombe

2017

Journal of Viral Hepatitis

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According to the Global Burden of Diseases, chronic viral hepatitis B and C are one of the most challenging global health conditions that rank among the first causes of morbidity and mortality worldwide. Low- and middle-income countries are particularly affected by the health burden associated with HBV or HCV infection. One major gap in efficiently addressing the issue of viral hepatitis is universal screening. However, the costs and chronic lack of human resources for using traditional screening strategies based on serology and molecular biology preclude any scaling-up. Point-of-care tests have been deemed a powerful potential solution to fill the current diagnostics gap in low-resource and decentralized settings. Despite high interest resulting from their development in recent years, very few point-of-care devices have reached the market. Scaling down and automating all testing steps in 1 single device (eg, sample preparation, detection and readout) is indeed challenging. But innovations in multiple disciplines such as nanotechnologies, microfluidics, biosensors and synthetic biology have led to the creation of chip-sized laboratory systems called "lab-on-a-chip" devices. This review aims to explain how these innovations can overcome technological barriers that usually arise for each testing step while developing integrated point-of-care tests. Point-of-care test prototypes rarely meet the requirements for mass production, which also hinders their large-scale production. In addition to logistical hurdles, legal and economic constraints specific to the commercialization of in vitro diagnostics, which have also participated in the low transfer of innovative point-of-care tests to the field, are discussed.

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A portable paper-based microfluidic platform for multiplexed electrochemical detection of human immunodeficiency virus and hepatitis C virus antibodies in serum

Cited by 77 publications

References 34 publications

Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

Paper-based diagnostic devices for clinical paraquat poisoning diagnosis

Recent advances in lab-on-a-chip technologies for viral diagnosis

Innovative technologies for point‐of‐care testing of viral hepatitis in low‐resource and decentralized settings

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