A possible role for nonsense suppression in the synthesis of a human cytomegalovirus 58-kDa virion protein

Lahijani, Roya S.; Otteson, Elmer W.; Jeor, Stephen C. St.

doi:10.1016/0042-6822(92)90087-6

Cited by 12 publications

(13 citation statements)

References 36 publications

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“…Earlier studies of this region using an antipeptide antibody (anti-P 2-1 ) had identified a 58-kDa late protein that was concluded to be the product of the 1.6-kb late mRNA from the pp28 gene region (16,17). This protein was proposed to be generated by way of a translational read-through because the coding region of the late 1.6-kb RNA was unable to encode a protein of 58 kDa (17). These studies were performed prior to the availability of the HCMV sequence, which subsequently revealed an ORF (UL98) that overlapped this region (3).…”

Section: Characterization Of I2-specific Proteins In Hcmv-infected Cementioning

confidence: 99%

The human cytomegalovirus UL98 gene transcription unit overlaps with the pp28 true late gene (UL99) and encodes a 58-kilodalton early protein

Adam

Jervey

Kohler

et al. 1995

J Virol

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A murine monoclonal antibody (I2) reacts strongly with the nucleus of human cytomegalovirus (HCMV)infected human fibroblasts. Western blot (immunoblot) analysis using I2 demonstrated that a protein with an apparent molecular mass of 58-kDa (E58) was expressed at 5 h after infection, and levels increased through 72 h. Immunoblot screening of an early cDNA expression library resulted in a positive clone which hybridized to the right end of the XbaI C fragment of the HCMV Towne strain. Further analysis demonstrated that the E58-specific clone was homologous to the putative UL98 open reading frame, which has been proposed to encode the viral alkaline exonuclease homolog. RNA analysis demonstrated a 3.0-kb RNA which is expressed at early times after infection, as well as in the absence of viral DNA replication, and which is 3 coterminal with the pp28 (UL99) gene region. Insertion of the UL98 genomic sequence into a eucaryotic expression vector and subsequent Western blot analysis using I2 demonstrated that the expressed protein comigrated with E58 from infected cells. E58 also reacts specifically with a previously described antibody, anti-P 2-1 , which was proposed to recognize a putative late 58-kDa protein. E58 comigrates with the putative late 58-kDa protein, indicating that these two proteins are likely the same. Analysis of the UL98 promoter revealed a TATATAA sequence located at nucleotide 142525. Insertion of the putative promoter 5 to a reporter gene demonstrated that the UL98 promoter was activated in cotransfection experiments with IE1 and IE2 proteins. These studies demonstrate that UL98 is a bona fide early gene, which is consistent with its probable role as the viral alkaline exonuclease gene.

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Section: Characterization Of I2-specific Proteins In Hcmv-infected Cementioning

confidence: 99%

The human cytomegalovirus UL98 gene transcription unit overlaps with the pp28 true late gene (UL99) and encodes a 58-kilodalton early protein

Adam

Jervey

Kohler

et al. 1995

J Virol

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“…Analysis of the HindIH R fragment identified two short ORFs (ORF2-1, ORF2-2) in addition to the ORF coding for ppUL99 (Lahijani et al, 1991(Lahijani et al, , 1992. Their initiation codon does not follow Kozak's rules which have been used to predict the potential number of ORFs in the HCMV genome (Chee et al, 1990).…”

Section: Internal Proteins With a Still Undefined Location (I) Pul98amentioning

confidence: 99%

“…A rabbit monospecific antiserum has been obtained against amino acids 12 to 26 ofppUL98a (Lahijani et al, 1991(Lahijani et al, , 1992) (see Table 1). …”

Section: Specific Immunological Reagentsmentioning

confidence: 99%

Human cytomegalovirus structural proteins

Spaete¹,

Gehrz²,

Landini³

1994

Journal of General Virology

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“…The translated protein migrates close to its predicted size of 65 kDa in SDS-PAGE gels, and has been reported to be phosphorylated and glycosylated in infected cells. Additionally, the UL98 protein is localized in the nucleus late in infection, and has been identified as a component of extracellular viral particles (Lahijani et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

The human cytomegalovirus UL98 gene encodes the conserved herpesvirus alkaline nuclease.

Sheaffer

Weinheimer

Tenney

1997

Journal of General Virology

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The human cytomegalovirus (HCMV) UL98 gene is predicted to encode a homologue of the conserved herpesvirus alkaline nuclease. To determine if the HCMV UL98 gene product is functionally homologous to other herpesvirus alkaline nucleases, the HCMV UL98 protein was purified and its activity characterized in vitro. Extracts of HCMV-infected cells were fractionated using Q Sepharose, phosphocellulose and native DNA cellulose chromatography. UL98 immunoreactivity copurified with alkaline pH-dependent nuclease activity. The purified protein migrated at its predicted size of approximately 65 kDa in denaturing polyacrylamide gels,

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A possible role for nonsense suppression in the synthesis of a human cytomegalovirus 58-kDa virion protein

Cited by 12 publications

References 36 publications

The human cytomegalovirus UL98 gene transcription unit overlaps with the pp28 true late gene (UL99) and encodes a 58-kilodalton early protein

The human cytomegalovirus UL98 gene transcription unit overlaps with the pp28 true late gene (UL99) and encodes a 58-kilodalton early protein

Human cytomegalovirus structural proteins

The human cytomegalovirus UL98 gene encodes the conserved herpesvirus alkaline nuclease.

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