A possible role for polyamines in cartilage in the mechanism of calcification

Vittur, Franco; Lunazzi, Gian Carlo; Moro, Luigi; Stagni, N.; Bernard, B. de; Moretti, Michèle; Stanta, Giorgio; Bacciottini, Franco; Orlandini, Giovanni; Reali, N; Casti, A

doi:10.1016/0304-4165(86)90094-2

Cited by 21 publications

(21 citation statements)

References 19 publications

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“…Polyamines are involved in a variety of cellular processes, including proliferation and differentiation, and several studies have implicated a role for polyamines in bone growth and development [28, 34]. Polyamines have also been suggested to play a role in the calcification of preosseous cartilage [28]. In epiphyseal cartilage from calf scapulas, polyamines were more abundantly present in the ossifying area as compared with the resting region [28].…”

Section: Discussionmentioning

confidence: 99%

“…Polyamines have also been suggested to play a role in the calcification of preosseous cartilage [28]. In epiphyseal cartilage from calf scapulas, polyamines were more abundantly present in the ossifying area as compared with the resting region [28]. In addition, alkaline phosphatase activity, which is a marker for bone cell differentiation, was enhanced by the addition of polyamines [28].…”

Section: Discussionmentioning

confidence: 99%

“…SSAT regulates polyamine catabolism, whereas polyamine modulating factor 1 (PMF‐1) modulates SSAT gene expression [23]. Polyamines are involved in various cellular processes, including proliferation, differentiation, apoptosis, and carcinogenesis [24, , –27], and have been implicated in bone growth and development [21, 28, –30]. Recent studies in our laboratory suggest a role for polyamines in the osteogenic differentiation of AT‐MSCs [31], indicating that polyamines may constitute an interesting tool for bone tissue engineering approaches targeting skeletal defects.…”

Section: Introductionmentioning

confidence: 99%

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Polyamines Modulate Nitric Oxide Production and Cox‐2 Gene Expression in Response to Mechanical Loading in Human Adipose Tissue‐Derived Mesenchymal Stem Cells

et al. 2006

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For bone tissue engineering, it is important that mesenchymal stem cells (MSCs) display a bone cell-like response to mechanical loading. We have shown earlier that this response includes increased nitric oxide (NO) production and cyclooxygenase-2 (COX-2) gene expression, both of which are intimately involved in mechanical adaptation of bone. COX-2 gene expression is likely regulated by polyamines, which are organic cations implicated in cell proliferation and differentiation. This has led to the hypothesis that polyamines may play a role in the response of adipose tissue-derived MSCs (AT-MSCs) to mechanical loading. The aim of this study was to investigate whether genes involved in polyamine metabolism are regulated by mechanical loading and to study whether polyamines modulate mechanical loading-induced NO production and COX-2 gene expression in human AT-MSCs. Human AT-MSCs displayed a bone cell-like response to mechanical loading applied by pulsating fluid flow (PFF), as demonstrated by increased NO production and increased gene expression of COX-2. Furthermore, PFF increased gene expression of spermidine/spermine N (1)-acetyltransferase, which is involved in polyamine catabolism, suggesting that mechanical loading modulates polyamine levels. Finally, the polyamine spermine was shown to inhibit both PFF-induced NO production and COX-2 gene expression, suggesting that polyamines modulate the response of human AT-MSCs to mechanical loading. In conclusion, this is the first study implicating polyamines in the response of human AT-MSCs to mechanical loading, creating opportunities for the use of polyamines in tissue engineering approaches targeting skeletal defects. STEM CELLS 2006;24:2262-2269

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polyamines Modulate Nitric Oxide Production and Cox‐2 Gene Expression in Response to Mechanical Loading in Human Adipose Tissue‐Derived Mesenchymal Stem Cells

et al. 2006

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“…Several studies have implicated polyamines in bone and cartilage growth and development [15–17]. Polyamines were shown to be abundantly present in the ossifying area as compared to the resting region in epiphyseal cartilage from calf scapulas [15], and alkaline phosphatase activity, which is a marker for bone cell differentiation, was enhanced by polyamines [15]. This suggests that polyamines may play a role in calcification of pre‐osseous cartilage.…”

Section: Introductionmentioning

confidence: 99%

The polymine spermine regulates osteogenic differentiation in adipose stem cells^†

Tjabringa

Zandieh-Doulabi

Helder

et al. 2008

J Cellular Molecular Medi

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For bone tissue engineering, it is important that mesenchymal stem cells (MSCs) differentiate into osteoblasts. To develop a method for differentiation of adipose tissue-derived mesenchymal stem cells (AT-MSCs) along the osteogenic lineage, we studied the effect of polyamines, which are organic cations implicated in bone growth and development, on differentiation of AT-MSCs. Treatment of goat-derived AT-MSCs with 1,25-dihydroxyvitamin-D3 (1,25(OH)2D3), which stimulates osteogenic differentiation, for 7 days induced gene expression of the polyamine-modulated transcription factor-1 (PMF-1) and spermidine/spermine N (1)-acetyltransferase (SSAT), which are both involved in polyamine metabolism, suggesting that polyamines are involved in osteogenic differentiation of AT-MSCs. Furthermore, treatment of AT-MSCs with the polyamine spermine-regulated gene expression of runx-2, a transcription factor involved in early stages of osteogenic differentiation, and that of osteopontin, a bone matrix protein expressed in later stages of osteogenic differentiation. Runx-2 gene expression was increased 4 and 14 days after a short 30 min. treatment with spermine, while osteopontin gene expression was only increased 4 days after spermine treatment. Finally, alkaline phosphatase activity, which is intimately involved in the formation of extracellular matrix of bone, was increased 4 weeks after the 30 min.-spermine treatment of AT-MSCs. In conclusion, this study shows for the first time that the polyamine spermine regulates differentiation of AT-MSCs along the osteogenic lineage, which can be used as a new method for differentiation of AT-MSCs along the osteogenic lineage. Therefore, polyamines may constitute a promising tool for bone tissue engineering approaches using AT-MSCs, such as a one-step surgical procedure for spinal interbody fusion.

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“…In contrast to the PHEX gene, which first appeared in teleosts (Bianchetti et al, 2002), both Sms and Sat are expressed in microorganisms (Tabor and Tabor, 1985). Moreover, a role for polyamines in skeletal function and mineralization remains to be determined (Vittur et al, 1986;Ljunggren et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

The X chromosome deletion in Hyp mice extends into the intergenic region but does not include the Sat gene downstream from Phex

Sabbagh

Gauthier

Tenenhouse³

2002

Cytogenet Genome Res

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The murine Hyp mutation is a model for X-linked hypophosphatemia (XLH), the most prevalent form of inherited rickets in humans. Although mutations in the murine Phex gene and the human PHEX gene have been identified in both murine and human disorders, the extent of the Hyp deletion on the mouse X chromosome has not been delineated. In the present study we demonstrate that the Hyp deletion starts in the middle of Phex intron 15 and includes ∼48 kb of the 3′ region of the Phex gene and ∼10 kb of intergenic sequence on the mouse X chromosome. In addition, we show that the Hyp deletion does not involve the downstream spermidine/spermine N1-acetyl transferase (Sat; formerly Ssat) gene and thus is not a contiguous gene deletion syndrome. Our data indicate that the Hyp mouse is a true homolog of XLH in humans and underscore the validity of this murine model in studies of XLH pathophysiology and for testing novel treatment modalities.

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A possible role for polyamines in cartilage in the mechanism of calcification

Cited by 21 publications

References 19 publications

Polyamines Modulate Nitric Oxide Production and Cox‐2 Gene Expression in Response to Mechanical Loading in Human Adipose Tissue‐Derived Mesenchymal Stem Cells

Polyamines Modulate Nitric Oxide Production and Cox‐2 Gene Expression in Response to Mechanical Loading in Human Adipose Tissue‐Derived Mesenchymal Stem Cells

The polymine spermine regulates osteogenic differentiation in adipose stem cells^†

The X chromosome deletion in <i>Hyp</i> mice extends into the intergenic region but does not include the <i>Sat</i> gene downstream from <i>Phex</i>

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A possible role for polyamines in cartilage in the mechanism of calcification

Cited by 21 publications

References 19 publications

Polyamines Modulate Nitric Oxide Production and Cox‐2 Gene Expression in Response to Mechanical Loading in Human Adipose Tissue‐Derived Mesenchymal Stem Cells

Polyamines Modulate Nitric Oxide Production and Cox‐2 Gene Expression in Response to Mechanical Loading in Human Adipose Tissue‐Derived Mesenchymal Stem Cells

The polymine spermine regulates osteogenic differentiation in adipose stem cells†

The X chromosome deletion in <i>Hyp</i> mice extends into the intergenic region but does not include the <i>Sat</i> gene downstream from <i>Phex</i>

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The polymine spermine regulates osteogenic differentiation in adipose stem cells^†