Recent puzzling observations, such as the H0 tension, large-scale anisotropies, and massive disk galaxies at high redshifts, have been challenging the standard cosmological model. While one possible explanation is that the standard model is incomplete, other theories are based on the contention that the redshift model as a distance indicator might be biased. These theories can explain the recent observations, but they are challenged by the absence of a direct empirical reproducible observation that the redshift model can indeed be inconsistent. Here, I describe a simple experiment that shows that the spectra of galaxies depend on their rotational velocity relative to the rotational velocity of the Milky Way. Moreover, it shows that the redshift of galaxies that rotate in the opposite direction relative to the Milky Way is significantly smaller compared with the redshift of galaxies that rotate in the same direction relative to the Milky Way (p < 0.006). Three different datasets were used independently, each one was prepared in a different manner, and all of them showed similar redshift bias. A fourth dataset of galaxies from the Southern Galactic pole was also analyzed and shows similar results. All four datasets are publicly available. While a maximum average z difference of ∼0.012 observed with galaxies of relatively low redshift (z < 0.25) is not extreme, the bias is consistent and canpotentially lead to explanations to puzzling observations such as the H0 tension.