A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT<sub>2</sub>- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs

Yamada, Yuko; Yamauchi, Daiki; Yokoo, Megumi; Ohinata, Kousaku; Usui, Hachiro; Yoshikawa, Masaaki

doi:10.1271/bbb.70638

Cited by 27 publications

(22 citation statements)

References 11 publications

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“…In endothelium-denuded artery, it did not show relaxing activity, indicating that the activity is mediated by an endothelium-derived relaxing factor. The relaxing effect of Novokinin was significantly blocked by AT2 receptor antagonist PD123319 (100 µM) suggests that the effects of vasodilator was mediated AT2 receptor [19]. The results of our study are similar to the literatures which showing hypotensive effect of Novokinin.…”

Section: Discussionsupporting

confidence: 90%

Effects of At2 Receptor Agonist Novokinin on Oxidative Stress, Inflammation and Infarct Size Due to Myocardial Ischemia-Reperfusion

E¹,

Şahna²

2017

JBiSE

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Aim: Ischemia and reperfusion (IR) injury is a serious problem that is occurred during thrombolytic therapy, organ transplantation, coronary angioplasty, and cardiopulmonary bypass. There is an increase in the number of AT2 receptors in some pathological conditions such as cardiac hypertrophy, myocardial infarction, congestive heart failure. This study was designed to investigate the effects AT2 receptor agonist Novokinin on infarct size, caveolin-1 (CAV-1), HSP90, ADMA, NADPH oxidase and Rhokinase associated to endothelial dysfunction and oxidative stress, and NFκB and TLR-4 levels induced by inflammation on myocardial IR. Methods: The experimental groups: Sham (C), Novokinin (N), IR and IRN. Novokinin was performed with infusion pump before ischemia, and during IR. The left main coronary artery was occluded for 30 minutes ischemia followed by 120 minutes reperfusion in anesthetized rats. CAV-1, HSP90, and NFκB levels were measured by the quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), ADMA, TLR-4, NADPH oxidase, and Rhokinase levels were measured by ELISA, infarct size was measured by ImageJ, an image analysis software, in the heart tissue. Results: NFκB, HSP90, NADPH oxidase and TLR-4 levels increased with IR and significantly decreased with Novokinin. CAV-1 levels were not different between the groups. ADMA and Rhokinase levels were increased due to IR but decreased with Novokinin. Novokinin reduced infarct size due to IR. Conclusion: Our results showed that, ADMA, HSP90, NFκB, TLR-4, Rhokinase, and NADPH oxidase levels play important roles on IR injury. AT2 receptor agonist Novokinin may affect positively oxidative changes, inflammation, and endothelial function in myocardial IR injury.

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Section: Discussionsupporting

confidence: 90%

Effects of At2 Receptor Agonist Novokinin on Oxidative Stress, Inflammation and Infarct Size Due to Myocardial Ischemia-Reperfusion

E¹,

Şahna²

2017

JBiSE

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“…In this context the hypotensive peptides ovokinin (2–7) (RADHPF) and novokinin (RPLKPW) have a similar structure but different vasorelaxing mechanisms. While NO and B2 bradykinin receptors are implicated in the mechanism of action of ovokinin,33, 37 the mechanism of novokinin is mediated by prostacyclin and the IP receptor downstream of the AT(2) receptor 38…”

Section: Resultsmentioning

confidence: 99%

Vascular effects of egg white-derived peptides in resistance arteries from rats. Structure-activity relationships

García-Redondo

Roque

Miguel

et al. 2010

J. Sci. Food Agric.

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“…For example, the antihypertensive peptides IPP and VPP can reduce blood pressure via the promotion of NO synthesis in endothelial cells . Novokinin reduced blood pressure via AT2 and IP receptor dependent mechanism . Therefore, it is possible that LRA lowered blood pressure not just by ACE inhibition alone, but further studies on its hypotensive activity are needed to elucidate any other mechanism.…”

Section: Discussionmentioning

confidence: 99%

A Novel Antihypertensive Peptide Identified in Thermolysin‐Digested Rice Bran

Shobako

Ogawa

Ishikado

et al. 2018

Molecular Nutrition Food Res

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A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT₂- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs

Cited by 27 publications

References 11 publications

Effects of At2 Receptor Agonist Novokinin on Oxidative Stress, Inflammation and Infarct Size Due to Myocardial Ischemia-Reperfusion

Effects of At2 Receptor Agonist Novokinin on Oxidative Stress, Inflammation and Infarct Size Due to Myocardial Ischemia-Reperfusion

Vascular effects of egg white-derived peptides in resistance arteries from rats. Structure-activity relationships

A Novel Antihypertensive Peptide Identified in Thermolysin‐Digested Rice Bran

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A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT2- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs

Cited by 27 publications

References 11 publications

Effects of At2 Receptor Agonist Novokinin on Oxidative Stress, Inflammation and Infarct Size Due to Myocardial Ischemia-Reperfusion

Effects of At2 Receptor Agonist Novokinin on Oxidative Stress, Inflammation and Infarct Size Due to Myocardial Ischemia-Reperfusion

Vascular effects of egg white-derived peptides in resistance arteries from rats. Structure-activity relationships

A Novel Antihypertensive Peptide Identified in Thermolysin‐Digested Rice Bran

Contact Info

Product

Resources

About

A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT₂- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs