A potential anti-tumor herbal medicine, Corilagin, inhibits ovarian cancer cell growth through blocking the TGF-β signaling pathways

Jia, Luoqi; Jin, Huajun; Zhou, Jiayi; Chen, Lianghua; Lu, Yiling; Ming, Yanlin; Yu, Yinhua

doi:10.1186/1472-6882-13-33

Cited by 100 publications

(96 citation statements)

References 27 publications

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“…The present study further identified that corilagin is a major active component from P. niruri L. extracts and has broadspectrum antitumor activity, a better antitumor potential but lower toxicity to normal cells (12). It is effective in retarding the growth of hepatocarcinoma cells by inducing G2/M phase arrest (9); inhibiting ovarian cancer cells via TGF-β/AKT/ERK signaling pathways (10); and suppressing cholangiocarcinoma progression through the Notch signaling pathway (11). Recently, we found that corilagin enhances the sensitizing effects of chemotherapy drugs in ovarian cancer cells, through Snail epithelial-mesenchymal transition (EMT) and glycolysis pathways.…”

Section: Introductionmentioning

confidence: 86%

Corilagin sensitizes epithelial ovarian cancer to chemotherapy by inhibiting Snail-glycolysis pathways

et al. 2017

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Abstract. We identified that corilagin is a major component extracted from a well-known hepatoprotective and antiviral medicinal herb, Phyllanthus niruri L with antitumor activity. Our previous study found that corilagin inhibited the growth of ovarian cancer cells via the TGF-β/AKT/ERK signaling pathways. Recently, we demonstrated that corilagin enhanced the sensitivity of ovarian cancer cells to chemotherapy. Ovarian cancer cell lines, SKOv3ip, Hey and HO-8910PM-Snail, were treated with different concentrations of corilagin in combination with paclitaxel and carboplatin. Corilagin distinctly enhanced the inhibitory effects of paclitaxel and carboplatin. To understand the mechanisms involved in the chemo-sensitization by corilagin, we performed reverse phase protein array analysis to determine the signaling networks induced by corilagin. We observed that both paclitaxel and carboplatin upregulated the expression levels of several apoptotic and death-related proteins, such as caspase 3, caspase 7 and PDCD4, which were further enhanced when combined with corilagin. Meanwhile, corilagin induced distinct pathways to paclitaxel and carboplatin treatment. We also performed isobaric tags for relative and absolute quantitation proteomics analysis in corilagen-treated ovarian cancer cells. This analysis indicated that corilagin is mainly involved in the glycolysis pathway. Seahorse XF96 extracellular acidification rate analysis confirmed that corilagin inhibited glycolysis by downregulation of CD44 and STAT3. In summary, our observations indicate that corilagin sensitized epithelial ovarian cancer cells to paclitaxel and carboplatin treatment by primarily inhibiting Snail-glycolysis pathways. Corilagin is a herbal medicine with low toxic effects to normal cells, particularly hepatoprotective, and may be an ideal complimentary medicine when combined with highly toxic chemotherapeutic agents. IntroductionCorilagin (C 27 H 22 O 18 ), a gallotannin found in many plants, is a major active component of many ethnopharmacological plants, such as Phyllanthus niruri L., P. emblica L. and P. urinaria L. Corilagin was first isolated in 1951 by Schmidt and Lademann from Divi-divi (Caesalpina coriaria) plants (1). In the past few decades, corilagin has been reported to display several pharmacological activities, including antioxidant (2), hepatoprotective (3), anti-inflammatory (4), neural system protective (5) and cardiovascular protective (6) activities, and has been found to be beneficial in managing type 2 diabetes (7). Recently, its antitumor effects in hepatocellular carcinoma (8,9), ovarian cancer (10) and cholangiocarcinoma (11) have attracted attention.Since 2005, we screened hundreds of herbs, among which Phyllanthus niruri L. has the highest anticancer potential. The present study further identified that corilagin is a major active component from P. niruri L. extracts and has broadspectrum antitumor activity, a better antitumor potential but lower toxicity to normal cells (12). It is effective in retarding the growth of he...

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Section: Introductionmentioning

confidence: 86%

Corilagin sensitizes epithelial ovarian cancer to chemotherapy by inhibiting Snail-glycolysis pathways

et al. 2017

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“…Flavonoids have demonstrated antiproliferative activity in prostate, renal, colon, non-Hodgkin's lymphoma, gastric carcinomas and neuroblastoma cancer cells [41][42][43]. Tannin isolated from Cuphea hyssopifolia, Phyllanthus niruri and Cistus ladanifer among other medicinal plants has been documented to have antiproliferative effects against breast, human promyelocytic leukemia HL-60, ovarian and pancreatic cancer cell lines [44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative Activity of Kenyan Trametes versicolor Aqueous Extract on Selected Cancer and Normal Cell Lines

JK¹,

Adipo²,

DM³

2016

J Cancer Sci Ther

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“…A tannin compound, Cuphiin D1 (CD1), isolated from Cuphea hyssopifolia has been proven to exert antiproliferative effects against human promyelocytic leukemia HL-60 cell line [31]. Corilagin, a tannin compound, isolated from a number of medicinal plants including Phyllanthus niruri has been shown to have antiproliferative effects against ovarian cancer cell lines [32]. Pancreatic cancer cells and breast cancer cells proliferation has been inhibited by Ellitannin compound isolated from the Cistus ladanifer [33].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antiproliferative Activity of Aqueous Root Bark Extract of Cassia abbreviata (Holmes) Brenan

SM¹,

RC²

2016

J Cancer Sci Ther

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Cancer is referred to as uncontrolled abnormal proliferation of body cells. Currently cancer treatment and management is a challenge due to complexity of the disease, toxicity of chemotherapy, unaffordability of treatment and severe side effects. Therefore, it is imperative to investigate complementary and alternative medicine for leads and development of anticancer drugs. Cassia abbreviate (C. abbreviata) has been traditionally used for its ethnotherapeutic properties and pharmacological activities. It has been reported to possess antiproliferative activity, though there is no scientific evidence to validate this claim. The main objective of this study was to determine the antiproliferative activity of aqueous root bark extract of C. abbreviata on hepatocellular carcinoma (HCC), vero, and macrophage cell lines in vitro. The antiproliferative activity of aqueous root bark extract of C. abbreviata was determined using MTT assay. The results showed that aqueous root bark extract of C. abbreviata has antiproliferative activity against HCC, vero and macrophage cell lines. The extract had the highest antiproliferative activity against HCC cell line with an IC 50 of 1.49 µg/ml as compared to 81.08 µg/ml and 128.38 µg/ml in macrophages and vero cells respectively. The IC 50 observed on non-cancerous normal cells (macrophages and vero cells) indicated that the plant extract had little antiproliferative effects on normal cells hence regarded as safe. The extract contained flavonoids, phenols, tannins and saponins. In conclusion the antiproliferative activity of aqueous root bark extract of Cassia abbreviata observed could be attributed to the phytochemicals present in this plant extract. The results of this study, validates the claim that aqueous root bark extract of C. abbreviata has antiproliferative activity and justifies its use in herbal medicine.

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A potential anti-tumor herbal medicine, Corilagin, inhibits ovarian cancer cell growth through blocking the TGF-β signaling pathways

Cited by 100 publications

References 27 publications

Corilagin sensitizes epithelial ovarian cancer to chemotherapy by inhibiting Snail-glycolysis pathways

Corilagin sensitizes epithelial ovarian cancer to chemotherapy by inhibiting Snail-glycolysis pathways

Antiproliferative Activity of Kenyan Trametes versicolor Aqueous Extract on Selected Cancer and Normal Cell Lines

In Vitro Antiproliferative Activity of Aqueous Root Bark Extract of Cassia abbreviata (Holmes) Brenan

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