A potential new technique for studying biofilm growth and stress resistance of <i>Listeria monocytogenes</i>, <i>Escherichia coli</i> O157:H7 and other bacterial species in industrial plant

Trémoulet, Frédéric; Clement, Estelle; Martinie, Brigitte Gaillard; Labadie, Jean-Claude

doi:10.3166/sda.22.305-317

Cited by 5 publications

(2 citation statements)

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“…The biofilms studied in this work were grown on GFF, according to a new and convenient approach[6] successfully used to identify proteins after 2‐D electrophoresis combined with MALDI‐TOF‐MS and database searching. The growth of E. coli O157:H7 on GFF at 20°C lasted between 24 and 48 h. After that time, the E. coli O157:H7 biofilms remained stable during 7 days and microscopical observations of the filters showed microcolonies in the 24‐h‐old biofilms and numerous bacteria embedded in exopolymers in those of 7 days (Fig.…”

Section: Discussionmentioning

confidence: 99%

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A proteomic study ofEscherichia coliO157:H7 NCTC 12900 cultivated in biofilm or in planktonic growth mode

Trémoulet¹,

Duché²,

Namane

et al. 2002

FEMS Microbiology Letters

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Escherichia coli 0157:H7 biofilms were studied by a new method of cultivation in order to identify some of the proteins involved in the biofilm phenotype. A proteomic analysis of sessile or planktonic bacteria of the same age was carried out by two-dimensional electrophoresis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and database searching. Comparison of two-dimensional gels showed clear differences between protein patterns of sessile and planktonic cells. Fourteen proteins increased in biofilms, whereas three decreased. From these 17 proteins, 10 were identified by MALDI-TOF-MS and could be classified into four categories according to their function: (1) general metabolism proteins (malate dehydrogenase, thiamine-phosphate pyrophosphorylase), (2) sugar and amino acid transporters (D-ribose-binding periplasmic protein, D-galactose-binding protein, YBEJ), (3) regulator proteins (DNA starvation protein and H-NS) and (4) three proteins with unknown function. The results of this study showed that E. coli O157:H7 modified the expression of several proteins involved in biofilm growth mode.

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Section: Discussionmentioning

confidence: 99%

“…Cultures were grown in a rotary shaker (150 rpm) at 20°C for 7 days. The glass fiber filter method was used to produce biofilms[6]. Briefly, biofilms were grown on a prefiltration membrane disk Glass Fiber Filter Extra Thick (GFF, Pall Bio Pharma, Ann Arbor, MI, USA) with 1 μm pore size, 25 mm diameter and 1270 μm thickness.…”

Section: Methodsmentioning

confidence: 99%