Investigation of the selective bactericidal effect of several decontaminating solutions on bacterial biofilms including useful, spoilage and/or pathogenic bacteria

Ammor, Salim; Chevallier, Isabelle; Laguet, Arlette; Labadie, Jean-Claude; Talon, Régine; Dufour, Éric

doi:10.1016/s0740-0020(03)00051-0

Cited by 28 publications

(22 citation statements)

References 17 publications

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“…These results are in agreement with those obtained by previous authors, who found a smaller log decrease for mixed L. monocytogenes biofilms exposed to disinfectants compared to the corresponding monospecies biofilm (Ammor et al, 2004).…”

Section: Discussionsupporting

confidence: 93%

“…It is widely recognized that one of the most frequent contamination routes of L. monocytogenes in foods is through cross contamination from the surfaces in food processing plants (Ammor et al, 2004;Norwood & Gilmour, 1999;Porsby, Vogel, Mohr, & Gram, 2008), where it is able to attach and form resistant biofilms (Aase, Sundheim, Langsrud, & Rørvik, 2000;Takahashi et al, 2009;To, Favrin, Romanova, & Griffiths, 2002). This resistance is related to the threedimensional structure of the biofilm, which defines the interactions between the bacterial species (Wuertz, Okabe, & Hausner, 2004) and determines the degree to which chemicals can access the cells (Bourion & Cerf, 1996;Qu, Daley, Istivan, Rouch, & Deighton, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Adherence kinetics, resistance to benzalkonium chloride and microscopic analysis of mixed biofilms formed by Listeria monocytogenes and Pseudomonas putida

et al. 2012

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Adherence kinetics, resistance to benzalkonium chloride and microscopic analysis of mixed biofilms formed by Listeria monocytogenes and Pseudomonas putida

et al. 2012

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“…The remaining cells were counted after scraping with two sterile moistened swabs and resuspended by vigorously vortexing in 9 mL of SPW. Serial dilutions were prepared in SPW, and aliquots of 100 µL were spread plated onto NA plates and incubated at 37 ºC for 24 h (Ammor et al, 2004). After the incubation period, the number of viable cells was counted and the results expressed as Log cfu/cm 2 .…”

Section: Sanitizer Applicationmentioning

confidence: 99%

“…Some pathogenic bacteria are able to adhere to food-contact surfaces and remain viable even after cleaning and disinfection (Ammor et al, 2004). One of the most common ways for bacteria to live is adhering to surfaces and forming biofilms in which they are embedded in an organic, extracellular polymeric matrix (Chae, Schraft, 2000).…”

Section: Introductionmentioning

confidence: 99%

Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

Jerônimo

Queiroga

Costa

et al. 2012

Braz. J. Pharm. Sci.

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This study assessed the effect of different growth media [BHI broth, BHI broth plus glucose (10 g/100 mL) and BHI broth plus NaCl (5 g/100 mL)] and incubation temperatures (28 or 37 ºC) on the adherence, detachment and biofilm formation on polypropylene and stainless steel surfaces (2 x 2 cm coupons) for a prolonged period (24-72 h) by some strains of Staphylococcus aureus (S3, S28 and S54) from food processing plants. The efficacy of the sanitizers sodium hypochlorite (250 mg/mL) and peracetic acid (30 mg/mL) in reducing the number of viable bacterial cells in a preformed biofilm was also evaluated. S. aureus strains adhered in highest numbers in BHI broth, regardless of the type of surface or incubation temperature. Cell detachment from surfaces revealed high persistence over the incubation period. The number of cells needed for biofilm formation was noted in all experimental systems after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered onto polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacities to adhere and form biofilms on polypropylene and stainless steel surfaces under the different growth conditions, and the cells in biofilm matrixes were resistant to total removal when exposed to the sanitizers sodium hypochlorite and peracetic acid. Uniterms:Staphylococcus aureus/food processing. Biofilm. Surfaces. Sanitizers/efficacy.Este estudo teve como objetivo avaliar o efeito de diferentes meios de crescimento [caldo BHI, caldo BHI adicionado de glucose (10 g/100 mL) e caldo BHI adicionado de NaCl (5 g/100 mL)] e temperaturas de incubação (28 e 37 °C) sobre a adesão, separação e formação de biofilme sobre superfícies (2 x 2 cm) de polipropileno e aço inoxidável durante longo tempo de incubação (24-72 h) por parte de cepas de Staphylococcus aureus (S3, S58 e S54) isoladas de plantas de processamento de alimentos. Também foi avaliada a eficácia dos sanitizantes hipoclorito de sódio (250 mg/mL) e ácido peracético (30 mg/mL) na redução do número de células bacterianas viáveis presentes em um biofilme pré-formado. As cepas de S. aureus aderiram em número mais elevado quando incubadas em caldo BHI em ambos os tipos de superfícies e temperaturas de incubação testadas. A separação das células das superfícies revelou alta persistência ao longo do período de incubação. Número de células necessário para a formação do biofilme foi detectado depois de três dias de incubação em todos os sistemas experimentais. O ácido peracético e o hipoclorito de sódio não foram eficientes em remover completamente a células de S. aureus aderidas sobre as superfícies de polipropileno e aço inoxidável. Os resultados obtidos revelaram alta capacidade das cepas ensaiadas em aderir e formar biofilme sobre superfícies de polipropileno e aço inoxidável sobre diferentes condições de crescimento e que as células na matriz do biofilme apresentaram-se resistentes à total remoção quando expostas aos sanitizantes hipoclorito de sód...

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“…Biofilms were grown according to the method of Ammor, Chevallier, Laguet, Labadie, and Talon (2004). Extra-thick fiberglass disks (diameter: 25 mm, Gelmann Sciences, Michigan, USA) were used as support matrix for biofilm growth.…”

Section: Biofilm Formationmentioning

confidence: 99%

Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility

et al. 2006

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Investigation of the selective bactericidal effect of several decontaminating solutions on bacterial biofilms including useful, spoilage and/or pathogenic bacteria

Cited by 28 publications

References 17 publications

Adherence kinetics, resistance to benzalkonium chloride and microscopic analysis of mixed biofilms formed by Listeria monocytogenes and Pseudomonas putida

Adherence kinetics, resistance to benzalkonium chloride and microscopic analysis of mixed biofilms formed by Listeria monocytogenes and Pseudomonas putida

Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature

Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility

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