A potyvirus P1 protein interacts with the Rieske Fe/S protein of its host

Abstract

Yeast two-hybrid (Y2H) screens were used to test for interactions between the P1 protein of Soybean mosaic virus Pinellia isolate (SMV-P) and a cDNA expression library of its host, the aroid Pinellia ternata. Of the 13 independent interacting clones identified, ten were identical and had an open reading frame predicted to encode a 23.7-kDa protein closely related to the cytochrome b6/f complex Rieske Fe/S genes of plants. The interaction between SMV-P-P1 and the mature Rieske Fe/S protein (without transit peptide) of the host was confirmed by in vitro co-immunoprecipitation of the two proteins. Y2H assays using different parts of the two proteins showed that only the N-terminal part (amino acids 1-82) of SMV-P P1 was responsible for the interaction with the Rieske Fe/S protein and that amino acids 1-33 interacted only with the transit peptide, while amino acids 34-82 could interact with the entire Rieske Fe/S protein. SMV-P P1 also interacted moderately with the Rieske Fe/S protein of its other hosts, soybean and Zantedeschia aethiopica, but weakly with that of the non-host Arabidopsis thaliana. The P1-Rieske Fe/S protein interactions are likely to be involved in symptom development, and the very variable N-terminus of P1 may play an important role in host adaptation.

Recombination and selection pressure in the ipomovirus sweet potato mild mottle virus (Potyviridae) in wild species and cultivated sweetpotato in the centre of evolution in East Africa

“…In comparison to a number of viruses in the family Potyviridae studied previously (reviewed by García-Arenal et al, 2001), SPMMV exhibited relatively high nucleotide diversity in the P1 region (Adams et al, 2005b). Data of previous studies suggest that P1 may be crucial for host adaptation in potyviruses (Shi et al, 2007;Salvador et al, 2008a). P1 proteins of potyviruses such as turnip mosaic virus and potato virus Y (PVY) have the largest v values among the mature viral proteins (at the population level), (Colinet et al, 1998) and E/P (Mukasa et al, 2003b) (broken arrows) are also shown.…”

Unfolding the secrets of plum pox virus: from epidemiology to genomics

“…P1 is clearly connected with the virus host specificity and symptom manifestation (Maliogka et al, 2012;Nagyová et al, 2012b). It binds RNA and interacts with the host chloroplast Rieske Fe/S protein (Shi et al, 2007). P1 cooperates with HC-pro in the inhibition of plant posttranscriptional gene silencing (PTGS) defence and its involvement in genome amplification has been presumed, too Verchot and Carrington, 1995).…”

Inducible expression of magnesium protoporphyrin chelatase subunit I (CHLI)-amiRNA provides insights into cucumber mosaic virus Y satellite RNA-induced chlorosis symptoms

“…These changes include (1) variation in chlorophyll fluorescence and reduced chlorophyll content [3], (2) inhibition of photosynthetic activity [17], (3) disproportionate accumulation of photoassimilates [1,23], (4) alterations in chloroplast ultrastructure and functions [4,25], (5) downregulation of nuclear-encoded chloroplast and photosynthesis-related genes and proteins (CPRGs and CPRPs, respectively) [8,21,34,35], (6) direct binding of viral components with chloroplast factors [4,33], and (7) perturbation of photosynthesis by regulation of host miRNA expression [43].…”