A Practical Approach to Clinical and Research Biobanking

Yong, William H.; Dry, Sarah M.; Shabihkhani, Maryam

doi:10.1007/978-1-4939-1050-2_8

Cited by 24 publications

(22 citation statements)

References 71 publications

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“…16,17 Whether tissue should be stored at -80°C or -140°C remains unclear. 13,23 We observed no adverse effects on RNA quality in fresh-frozen tissue after storage at -80°C for up to 6 years.…”

Section: Discussionmentioning

confidence: 77%

“…13 We observed a small but significant difference in RIN between laparoscopic and open procedures for normal tissue but not tumor tissue. The mean operation time tends to be longer with laparoscopic colon surgery; 29 however, in this study, we only measured the time from the clamping of the vessels to the retrieval of the biopsy.…”

Section: Discussionmentioning

confidence: 88%

“…These factors include in and ex vivo ischemic time, storage time, type of anesthesia, pathological features, and co-morbidity. 13 In this study, we investigated the feasibility of obtaining high-quality tissue from a colon cancer biobank for downstream RNA analyses. Furthermore, we investigated the impact of in vivo ischemic time, storage time, pathological characteristics, and surgical procedure on RNA quality.…”

Section: Introductionmentioning

confidence: 99%

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Impact of in Vivo Ischemic Time on RNA Quality—Experiences from a Colon Cancer Biobank

Olsen

Eiholm

et al. 2015

Biopreservation and Biobanking

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Considerable effort has been made to improve differentiated diagnostics as well as personalized treatment for colorectal cancer patients. High-quality fresh frozen tissue is often required to investigate relevant molecular signatures in these patients. In RNA expression studies, the "RNA integrity number" is widely accepted as a reliable marker of RNA quality. Here, we investigate the feasibility of obtaining high-quality tissue from a colon cancer biobank and the impact of in vivo ischemic time and various technical and clinicopathological factors on RNA quality. Biopsies were obtained immediately following the tumor removal. The time from clamping the main arterial supply to resection and removal of the tumor was used to estimate the in vivo ischemic time. We did not observe a significant difference in RNA quality between normal tissue and tumor tissue. We observed a significant correlation between in vivo ischemic time and RNA quality in normal tissue (r = -0.24, p<0.001) but not in tumor tissue. Male gender and laparoscopic procedure were also significantly associated with lower RNA quality in normal tissue only. In tumor tissue, poor differentiation was associated with low RNA quality. In conclusion, in vivo ischemic time, surgical procedure, and gender have minor but significant effects on the quality of RNA from normal colon tissue but not tumor tissue. Poorly differentiated tumors are associated with lower RNA quality. Although its impact is low, it can still be considered to note in vivo ischemic time in colon cancer specimen procurement.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Impact of in Vivo Ischemic Time on RNA Quality—Experiences from a Colon Cancer Biobank

Olsen

Eiholm

et al. 2015

Biopreservation and Biobanking

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“…All standard operating procedures followed are based on NFS 96-900 Quality of Biological Resource Centers (BRC) -French management system of a BRC and quality of biological resources of human and microbial origin. [4][5][6] …”

Section: Standard Operating Proceduresmentioning

confidence: 99%

Biobank-based research on digestive peritoneal carcinomatosis (the BIG-RENAPE Biobank)

Villeneuve¹,

Jourdan-Enfer²,

Bibeau³

et al. 2016

J Peritoneum

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Access to good quality biological samples is a prerequisite for highlevel translational research. The BIG-RENAPE Biobank has been established by the French hyperthermic intraperitoneal chemotherapy centers involved in the management of peritoneal surface malignancies. The Biobank is a core facility aiming to support researchers who conduct studies with sample collections, both within and outside the Big-RENAPE Network.The Biobank is certified according to NFS 96-900 and has been launched in February 2016 as a service of processing, storage and transfer of high quality biological (plasma, serum, buffy coat) and tissue (formalin-fixed-paraffin-embedded) samples from patients with digestive peritoneal carcinomatosis. Biospecimens are collected at each stage of diagnostic and therapeutic care. The patient and his derivates are anonymized and registered in a web database reporting disease status, treatments, surgical procedures, pathological diagnosis, quality of life's assessment and long term follow-up. All participants have given their informed consent before any sample. The Biobank was approved by the local Ethical Committee, based on the assessed compliance to French regulatory rules. The Biobank is located in the Centre Hospitalier Lyon (SudBioTech), which is known to be an expert center in the management of the peritoneal carcinomatosis.Research projects that require material stored in the Biobank are submitted by specific form and evaluated by the BIG-RENAPE Scientific Committee. A material transfer agreement is agreed with the recipient before samples are allowed to be sent from the Biobank.

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“…6,7 Standard Operating Procedures (SOPs) have been developed to ensure sample quality and minimize variability in research results. The SOPs draw on the Best Practices issued by ISBER and other organizations, which provide recommendations for standard workflows and operating procedures.…”

Section: Introductionmentioning

confidence: 99%

Practical Electronic Information System and Printed Recording Promote Management Accuracy in an Early-Stage Small-Scale Non-Automatic Biobank

Gao

Gu²,

Lv³

et al. 2015

Biopreservation and Biobanking

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It is particularly necessary for biomedical researchers to obtain applicable biosamples accurately and efficiently, especially from a biobank with multiple-disease catalogs. To optimize the retrieval procedure, especially in the early stages of a non-automatic biobank, we developed a procedure that combined the electronic information system with a graphically designed printed recording system, which assisted in retrieving the samples quickly in a visualized way. In this procedure, we designed tables depending on the structure of equipment and registered the corresponding information in the tables layer by layer. Different samples from different types of diseases were first registered in the electronic system with the specific pre-allocation and barcodes. Then they were stored in the allocated position using their respective barcodes. In this way, the sample number and the location information in the electronic database were completely matched with the printed record. When the samples are needed, it is convenient to check the electronic information with the printed record. This procedure provides a convenient way to record the sample information during its lifecycle, and helps the administrator to double check information about the sample. The current solution offers an easy way for the transformation of a non-automatic biobank from the small-scale early-stage to the large-scale highly-automated level.

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A Practical Approach to Clinical and Research Biobanking

Cited by 24 publications

References 71 publications

Impact of in Vivo Ischemic Time on RNA Quality—Experiences from a Colon Cancer Biobank

Impact of in Vivo Ischemic Time on RNA Quality—Experiences from a Colon Cancer Biobank

Biobank-based research on digestive peritoneal carcinomatosis (the BIG-RENAPE Biobank)

Practical Electronic Information System and Printed Recording Promote Management Accuracy in an Early-Stage Small-Scale Non-Automatic Biobank

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