A practical guide to hydrogels for cell culture

Caliari, Steven R.; Burdick, Jason A.

doi:10.1038/nmeth.3839

Cited by 1,557 publications

(1,407 citation statements)

References 98 publications

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“…Hydrogels, networks of polymers that have been swollen with water, are attractive materials for cellular applications due to their biocompatibility, ease of fabrication, and capacity for customization (Caliari and Burdick, 2016). One advantage of hydrogels is that their porosity is not detrimental to their structure and can allow for migration of cells within the hydrogel scaffold.…”

Section: Topographical Cues Drive Alignment and Directionalitymentioning

confidence: 99%

Biomaterials for Enhancing Neuronal Repair

Cangellaris

Gillette

2018

Front. Mater.

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As they differentiate from neuroblasts, nascent neurons become highly polarized and elongate. Neurons extend and elaborate fine and fragile cellular extensions that form circuits enabling long-distance communication and signal integration within the body. While other organ systems are developing, projections of differentiating neurons find paths to distant targets. Subsequent post-developmental neuronal damage is catastrophic because the cues for reinnervation are no longer active. Advances in biomaterials are enabling fabrication of micro-environments that encourage neuronal regrowth and restoration of function by recreating these developmental cues. This mini-review considers new materials that employ topographical, chemical, electrical, and/or mechanical cues for use in neuronal repair. Manipulating and integrating these elements in different combinations will generate new technologies to enhance neural repair.

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Section: Topographical Cues Drive Alignment and Directionalitymentioning

confidence: 99%

Biomaterials for Enhancing Neuronal Repair

Cangellaris

Gillette

2018

Front. Mater.

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“…The interest in these materials mainly relies on the fact that their composition and structure resembles the ECM properties of native tissues [26]. These polymers can also be easily converted into either physical or chemical hydrogels under cell-compatible conditions through a myriad of crosslinking pathways [210].…”

Section: Hydrogel Bioinksmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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“…degradation rate, porosity, and mechanical properties). Adapted by permission from Macmillan Publishers Ltd: [NATURE METHODS] [7] , copyright (2016).…”

Section: Neural Tissue Engineering: the Need For Relevant Three-dimenmentioning

confidence: 99%

Three-dimensional neuronal cell culture: in pursuit of novel treatments for neurodegenerative disease

et al. 2017

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To gain a better understanding of the underlying mechanisms of neurological disease, relevant tissue models are imperative. Over the years, this realization has fuelled the development of novel tools and platforms, which aim at capturing in vivo complexity. One example is the field of biofabrication, which focuses on fabrication of three-dimensional (3D) biologically functional products in a controlled and automated manner. Herein, we provide a general overview of classical 3D cell culture platforms, particularly in the context of neurodegenerative disease. Subsequently, the focus is put on bioprinting-based biofabrication, its potential to advance 3D neuronal cell culture and, to conclude, the relevant translational bottlenecks, which will need to be considered as the field evolves.

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A practical guide to hydrogels for cell culture

Cited by 1,557 publications

References 98 publications

Biomaterials for Enhancing Neuronal Repair

Biomaterials for Enhancing Neuronal Repair

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

Three-dimensional neuronal cell culture: in pursuit of novel treatments for neurodegenerative disease

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