A practical protocol for particle bombardment-mediated transformation of Phyllostachys bamboo suspension cells

Ogita, Shinjiro; Kikuchi, Nanaka; Nomura, Taiji; Kato, Yasuo

doi:10.5511/plantbiotechnology.10.1101a

Cited by 31 publications

(43 citation statements)

References 20 publications

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“…The monopodial rhizome structure of the running bamboos is long and the rhizomes spread quickly, making it a top choice for reforestation and industrial cultivation. There are fewer successful and convenient plantlet regeneration systems for monopodial bamboo [19]–[22]. It is necessary to establish simple and cost-effective plantlet regeneration in monopodial bamboos.…”

Section: Introductionmentioning

confidence: 99%

Protocol for Callus Induction and Somatic Embryogenesis in Moso Bamboo

Yuan

Yue

et al. 2013

PLoS ONE

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Moso bamboo [Phyllostachys heterocycla var. pubescens (Mazel ex J. Houz.) Ohwi] is one of the most important forest crops in China and the rest of Asia. Although many sympodial bamboo tissue culture protocols have been established, there is no protocol available for plantlet regeneration as indicated by callus induction for monopodial bamboos, such as Moso bamboo. In the present report, embryogenic callus induction, embryoid development, and germination were established for Moso bamboo from zygotic seed embryos. Callus was initiated from zygotic embryos after 10–20 d culture on MS media supplemented with 4.0 mg/L 2, 4-D and 0.1 mg/L zeatin (ZT). About 50% of the explants produced calli, and nearly 15% of the calli were found to be embryogenic in nature. These embryogenic calli can be subcultured for proliferation in the Murashige and Skoog media (MS) supplemented with 0.5–2.0 mg/L 2, 4-D. These calli were found to have maintained their capacity for regeneration even after one year of subculture. The viable somatic embryoids regenerated in medium containing 5.0–7.0 mg/L ZT. Nearly 5% of the calli were found capable of regenerating into plantlets directly in MS medium containing 5.0–7.0 mg/L ZT. Root growth was more pronounced when the plantlets were transferred to medium containing 2.0 mg/L NAA. After 30 days of subculture, the plantlets were transferred to a greenhouse.

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Section: Introductionmentioning

confidence: 99%

Protocol for Callus Induction and Somatic Embryogenesis in Moso Bamboo

Yuan

Yue

et al. 2013

PLoS ONE

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“…Cells in these cultures proliferate in a highly synchronous manner and show uniform morphological features (Ogita 2005;Ogita et al 2012c). We also established highly efficient particle bombardment transformation protocols for suspension-cultured cells of P. nigra and P. bambusoides (Ogita et al 2011(Ogita et al , 2012a. To study the sequential biological events in xylogenesis in living bamboo cells, we developed a culture system that promotes a high degree of lignification in P. nigra suspension cells (Ogita et al 2012b).…”

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confidence: 99%

“…To study the sequential biological events in xylogenesis in living bamboo cells, we developed a culture system that promotes a high degree of lignification in P. nigra suspension cells (Ogita et al 2012b). In parallel, we established culture conditions that promote rapid cell proliferation without lignin deposition (Ogita et al 2011). Those culture systems enabled us to explore the mechanisms of xylogenesis in bamboo cells by comparative analyses of cells in distinct developmental phases, i.e., proliferation and lignification.…”

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confidence: 99%

Occurrence of hydroxycinnamoylputrescines in xylogenic bamboo suspension cells

Nomura

Shiozawa

Ogita

et al. 2013

Plant Biotechnology

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To explore the chemical basis of xylogenesis in bamboo cells, we compared secondary metabolite compositions between xylogenic and non-xylogenic suspension cell cultures of bamboo (Phyllostachys nigra), which we developed previously. Two compounds, one major and one minor, showed large-scale increases in the cells cultured under two lignification (xylogenic) conditions, compared with cells cultured under proliferation (non-xylogenic) conditions. Based on spectroscopic analyses, the major compound was identified as feruloylputrescine (FP) and the minor compound was identified as p-coumaroylputrescine (pCP). We examined the accumulation profiles of these hydroxycinnamic acid amides during a 16-day culture period. When cells were kept in proliferation conditions for 16 days, the contents of FP and pCP peaked at 2 days (0.32 and 0.25 nmol mg −1 fresh weight, respectively) and decreased to trace levels thereafter. In contrast, the FP content increased throughout the 16-day culture period, reaching maximum levels of 4.3 and 6.8 nmol mg −1 fresh weight in the two xylogenic conditions. The pCP content was lower than that of FP under both xylogenic conditions. The pattern of FP accumulation resembled that of lignin accumulation, as monitored by phloroglucinol-HCl staining. It is likely that FP plays a role in xylogenesis in suspension-cultured bamboo cells.

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“…Changes in SCV and FW during the culture period were monitored as shown in pattern as previously documented [8]. There was a lag growth phase for ca.…”

Section: Growth Patterns Of Suspension Cellsmentioning

confidence: 99%

“…Changes in the SCV value were monitored during the 14 days of culture period. The SCV was measured by holding a liquid suspension cells for 15 min in a 50 ml centrifugation tube graduated in milliliters [8].…”

Section: Growth Regulation Of Suspension Cellsmentioning

confidence: 99%

A <i>β</i>-Glucosidase Activity Potentially Involved in Cell Division and Wall Development of <i>Phyllostachys</i> Bamboo Suspension Cells

Ogita¹,

Ohki²,

Nomura³

et al. 2012

AJPS

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We propose a novel Madake (Phyllostachys bambusoides) bamboo suspension culture model for investigation of key enzyme(s) activity involved in growth/differentiation. Sedimented Cell Volume (SCV) and fresh weight (FW) of the suspension cultured cells reached 34% (v/v) and 8.7 g in 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D)-containing medium while only 7% (v/v) SCV and 1.9 g FW of the cells in 10 μM gibberellic acid (GA 3 )-containing medium in 14 days. Proportion of mitotically active cells (S to G 2 /M phases) at a log phase was identified as 29.5% in the former cells with tiny cytoplasmic features while 5.4% in the latter cells with elongation, wall thickening, and lignification by using flow cytometry and laser scanning microscopic analysis. The total β-glucosidase (BGL) activity under the 2,4-D condition increased from 4.8 U in day 2 to 26.2 U in day 14 (ca. 5.5-fold) while a slight reduction, from 4.4 U in day 2 to 2.1 U in day 14 (ca. 0.5-fold), occurred when cell division was suppressed under the GA 3 condition. Ratio of the BGL activity of the soluble fractions to the membrane-associated fractions varied depending of the culture condition. The ratio was stable (2 to 8) during the culture period under the 2,4-D condition. Interestingly, the activity of the soluble enzyme fractions increased up to ca. 65% under the GA 3 condition in inverse proportion to the membrane-associated enzymes. All together, it was strongly suggested that the detected specificity/variability of BGL activity is potentially involved in cell division and lignification in Madake bamboo cells.

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A practical protocol for particle bombardment-mediated transformation of Phyllostachys bamboo suspension cells

Cited by 31 publications

References 20 publications

Protocol for Callus Induction and Somatic Embryogenesis in Moso Bamboo

Protocol for Callus Induction and Somatic Embryogenesis in Moso Bamboo

Occurrence of hydroxycinnamoylputrescines in xylogenic bamboo suspension cells

A <i>β</i>-Glucosidase Activity Potentially Involved in Cell Division and Wall Development of <i>Phyllostachys</i> Bamboo Suspension Cells

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A practical protocol for particle bombardment-mediated transformation of Phyllostachys bamboo suspension cells

Cited by 31 publications

References 20 publications

Protocol for Callus Induction and Somatic Embryogenesis in Moso Bamboo

Protocol for Callus Induction and Somatic Embryogenesis in Moso Bamboo

Occurrence of hydroxycinnamoylputrescines in xylogenic bamboo suspension cells

A &lt;i&gt;β&lt;/i&gt;-Glucosidase Activity Potentially Involved in Cell Division and Wall Development of &lt;i&gt;Phyllostachys&lt;/i&gt; Bamboo Suspension Cells

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A <i>β</i>-Glucosidase Activity Potentially Involved in Cell Division and Wall Development of <i>Phyllostachys</i> Bamboo Suspension Cells