A pre-clinical evaluation of silver, iodine and Manuka honey based dressings in a model of traumatic extremity wounds contaminated with Staphylococcus aureus

Guthrie, H C; Martin, Kevin R.; Taylor, Christopher J.; Spear, Abigail M.; Whiting, Rachel; Macildowie, Sara; Clasper, J; Watts, Sarah

doi:10.1016/j.injury.2014.05.007

Cited by 18 publications

(10 citation statements)

References 58 publications

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“…Guthrie and colleague [66] examined commercially available dressings: Inadine, Acticoat, and Activon Tulle in rabbit model of contaminated (by S. aureus) forelimb muscle injury. A positive control group was treated with antibiotics.…”

Section: Wound Dressing Containingmentioning

confidence: 99%

Certain Aspects of Silver and Silver Nanoparticles in Wound Care: A Minireview

Konop

Damps

Misicka

et al. 2016

Journal of Nanomaterials

133

106

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Resistance to antimicrobial agents by pathogenic bacteria has emerged in recent years and is a major health problem. In this context silver and silver nanoparticles (AgNP) have been known to have inhibitory and bactericidal effects and was used throughout history for treatment of skin ulcer, bone fracture, and supporting wound healing. In all of these applications prevention and treatment of bacterial colonized/infected wounds are critical. In this context silver and its derivatives play an important role in health care. Silver is widely used in clinical practice in the form of silver nitrate and/or silver sulfadiazine. In the last few years silver nanoparticles entered into clinical practice as both antimicrobial and antifungal agents. In addition, nanosilver is used in coating medical devices (catheters) and as component of wound dressings. In this paper we present summarized information about silver and nanoparticles made of silver in the context of their useful properties, especially antibacterial ones, being of a great interest for researchers and clinicians.

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Section: Wound Dressing Containingmentioning

confidence: 99%

Certain Aspects of Silver and Silver Nanoparticles in Wound Care: A Minireview

Konop

Damps

Misicka

et al. 2016

Journal of Nanomaterials

133

106

View full text Add to dashboard Cite

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“…By contrast, MH, made from the nectar of the manuka bush (Leptospermum scoparium), remains active after catalase treatment and is effective at low concentrations, and is therefore much more effective as an antimicrobial agent (Molan and Russell 2015). Manuka honey has proven effective at both the treatment and prevention of growth of multi-drug resistant bacteria (Jenkins et al 2011a), fungi (Irish et al 2006) and biofilms (Maddocks et al 2012); this has made it a suitable alternative to common antimicrobial treatments, especially in wound care (Guthrie et al 2014;Abd El-Malek et al 2017;Watson et al 2017;Hussain 2018;Szweda et al 2018). There are many factors contributing to the antimicrobial activity of MH, including low pH, high osmolarity and the presence of phytochemicals (Mandal and Mandal 2011).…”

Section: Introductionmentioning

confidence: 99%

Manuka honey chelates iron and impacts iron regulation in key bacterial pathogens

et al. 2019

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Aim The aim of this study was to test the hypothesis that Manuka honey (MH) chelates iron and promotes an iron‐limiting environment, which contributes to its antimicrobial activity. Methods and Results Employing a ferrozine‐based assay, we observed that MH is an iron chelator that depletes iron from solution. Siderophores are small molecules that bind ferric iron (III) with high affinity and their levels are upregulated by bacteria under iron‐limiting conditions. We demonstrated by quantitating siderophore production that Escherichia coli and Pseudomonas aeruginosa treated with MH sub‐minimum inhibitory concentrations (sub‐MIC) experience an iron‐limiting environment and increase siderophore production. In addition, supplementation with ferrous iron (II) significantly increased growth of E. coli, Staphylococcus aureus and P. aeruginosa cultured at their MH MIC above that observed in nonsupplemented controls. By contrast, supplementation with ferric iron (III) significantly increased growth for only E. coli and P. aeruginosa, above their nonsupplemented controls. Conclusions Manuka honey chelates iron, thereby generating an iron‐limiting environment for E. coli and P. aeruginosa, and to a lesser extent S. aureus, which contributes to its antimicrobial properties. Significance and Impact of the Study Our work demonstrates that MH‐induced iron chelation is an antimicrobial mechanism that differentially impacts the bacterial species tested here. Iron chelation affects multiple diverse physiological processes in bacteria and would contribute to the lack of bacterial resistance to MH. Iron metabolism is tightly regulated; bacteria require this essential nutrient for survival, but in excess it is toxic. Additional exploration of MH’s iron chelation mechanism will facilitate its future use in mainstream medicine.

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“…The uptake of various species was studied to assess the potential of the hydrogels for environmental applications (e.g., waste water purification [41][42][43][44][45][46][47]) or biomedical applications (e.g., wound dressings [3,[48][49][50][51][52]).…”

Section: Uptake and Release Of Species From Hydrogelsmentioning

confidence: 99%

Uptake and Release of Species from Carbohydrate Containing Organogels and Hydrogels

Pan

Roy

Haldar

et al. 2019

Gels

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Hydrogels are used for a variety of technical and medical applications capitalizing on their three-dimensional (3D) cross-linked polymeric structures and ability to act as a reservoir for encapsulated species (potentially encapsulating or releasing them in response to environmental stimuli). In this study, carbohydrate-based organogels were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization of a β-D-glucose pentaacetate containing methacrylate monomer (Ac-glu-HEMA) in the presence of a di-vinyl cross-linker; these organogels could be converted to hydrogels by treatment with sodium methoxide (NaOMe). These materials were studied using solid state 13C cross-polarization/magic-angle spinning (CP/MAS) NMR, Fourier transform infrared (FTIR) spectroscopy, and field emission scanning electron microscopy (FE-SEM). The swelling of the gels in both organic solvents and water were studied, as was their ability to absorb model bioactive molecules (the cationic dyes methylene blue (MB) and rhodamine B (RhB)) and absorb/release silver nitrate, demonstrating such gels have potential for environmental and biomedical applications.

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A pre-clinical evaluation of silver, iodine and Manuka honey based dressings in a model of traumatic extremity wounds contaminated with Staphylococcus aureus

Cited by 18 publications

References 58 publications

Certain Aspects of Silver and Silver Nanoparticles in Wound Care: A Minireview

Certain Aspects of Silver and Silver Nanoparticles in Wound Care: A Minireview

Manuka honey chelates iron and impacts iron regulation in key bacterial pathogens

Uptake and Release of Species from Carbohydrate Containing Organogels and Hydrogels

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