A predictive nutritional model for plant cells and hairy roots

Abstract

A structured nutritional model is proposed to describe growth and nutritional behavior of Eschscholtzia californica suspension cells and Catharanthus roseus and Daucus carota hairy roots in in vitro culture. The model describes the cells specific growth rate from concentration of intracellular nutrients such as inorganic phosphate (Pi), nitrogen sources (NO(3) (-) and NH(4) (+)) and sugars. Two-level Michaelis-Menten kinetics are used to describe Pi and NO(3) (-) uptake and simple Michaelis-Menten kinetics for description of sugars uptake. Model parameters for each cell line were calibrated using data from batch cultures. The predictive capacity of the model was tested using data from medium exchange hairy root cultures. The model describes growth and nutritional behavior for the cell and hairy root lines. A sensitivity analysis was performed to identify critical model parameters and effect of initial conditions. The cell and hairy roots lines are also compared from their kinetic parameters. The kinetic model is efficient for describing and predicting growth and nutritional behaviors of suspension cells and hairy roots.

A new approach to define optimized range of medium composition for enhancement of hairy root production in fed-batch process

“…The roots probably use their mineral stock from previous culture. In this case, the intracellular nutrient level determine growth rate [22]. Nevertheless, if the nutrients are correctly supplemented during a fed-batch operation, we can suppose that the intracellular nutrient levels are quasi constant near the saturation.…”

Hairy root biotechnology—indicative timeline to understand missing links and future outlook

“…An accurate picture of 'culture in real time' at any scale, stage, or condition would be immensely helpful in eliminating the possible limitations during the growth. Modeling the tissue growth by measuring osmolality (concentrations of different components) and the conductivity of the medium during culture are the examples of mathematical estimation of root growth through fresh weight time course data (Leduc et al 2006;Cloutier et al 2008). Another relevant example that uses the mathematical calculation to study the biomass increment based on population balance approach has also been proposed (Han et al 2004).…”

Plant cell culture technology–harnessing a biological approach for competitive cyclotides production

“…While organ, e.g. shoot and root, cultures offer genetic stability as well as, in some instances, superior performance over suspension cultures of the same line, the development of appropriate reactors and processing techniques for these systems involves enormous investment (Cloutier et al 2008;Guillon et al 2006;Mishra and Ranjan 2008).…”