A preliminary evaluation of the use of microbial culture filtrates for the control of contaminants in plant tissue culture systems

Hussain, Syed Mazahir; Lane, S.D.; Price, David

doi:10.1007/bf00048314

Cited by 13 publications

(5 citation statements)

References 16 publications

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“…Using NS may be more convenient and less toxic than using antibiotics in the medium. Furthermore, using other methods for controlling the infection like first acidification of the medium and later regulation of pH to normal condition (Leifert et al 2000) and microbial culture filtrate (Hussain et al 1994) may be time consuming methods in tissue culture techniques. Showing acceptable antibacterial activity in this investigation is in agreement with the results obtained by other investigators (Nomiya et al 2004;Sondi and Salopek-Sondi 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Fungi and bacteria are the most common microorganisms to be found on or in plant tissues. To eliminate bacterial contamination during in vitro propagation, different methods have been developed in the last few years (Barrett and Cassells 1994;Hussain et al 1994;Herman 1996). Antibiotics are commonly used in the medium to eliminate unwanted contaminants from plant systems (Smart et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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Nano silver: a novel nanomaterial for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture

2008

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Bacterial contamination is a serious problem in plant tissue culture procedures. An experiment was conducted to evaluate the potential of nano silver (NS) to remove bacterial contaminants of valerian nodal explants. This experiment was conducted as a completely randomized design in a factorial arrangement with four replications and each replicate with ten explants. Treatments involved NS at two stages (before and after surface sterilization along with control) with three rates (25, 50 and 100 mg l -1 ) at three times of soaking (30, 60 and 180 min). Explants were cultured on MS medium supplemented with 5 mg l -1 Kin and 0.1 mg l -1 NAA. Results showed that using 100 mg l -1 of NS solution after surface sterilization resulted in the highest percentage (89%) of disinfected explants. Nano silver solution did not affect the characters measured. On the basis of the data obtained in this experiment, it was concluded that NS had a good potential for removing of the bacterial contaminants in plant tissue culture procedures. As this is the first report on application of NS in in vitro culture techniques, further investigations on other plant species are needed to clarify the effectiveness of NS for the removal of bacterial contaminants in tissue culture of other crops.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nano silver: a novel nanomaterial for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture

2008

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“…The use of NS can be more comfortable and less toxic than using antibiotics in the medium. Furthermore, the use of other methods of controlling infection as the first medium acidification and subsequent pH adjustment to normal conditions (Leifert et al, 2000) and filtering the microbial culture (Hussain et al, 1994) may be methods slow in tissue culture techniques. To show acceptable antibacterial activity in this study is consistent with the findings of other researchers (Nomiya et al, 2004, Sondi andSalopek-Sondi 2004).…”

Section: Resultsmentioning

confidence: 99%

Effect of Agno3 Nanoparticles on Sterilization and Alkaloid Production of Catherenthus Roseus Tissue By

Shahin

2018

Al-Azhar Journal of Pharmaceutical Sciences

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Bacterial contamination is a serious problem in plant tissue culture procedures. An experiment has been conducted to evaluate the potential of nanosilver (NS) to remove bacterial contaminants from Catherenthus shoot tips and single nodes. The experiments were performed as a totally randomized design in a faculty with four replications and each replicate with ten explants. Treatments involved NS in two steps (before and after surface sterilization along with control) at three concentrations (25, 50 and 100 mg 1-1) at three times (30, 45 and 60 minutes). Plants were grown on MS medium supplemented with 5 mg l-1 of Kinetin (KIN) and 0.1 mgl-1 of naphthaline acetic acid (NAA). The results showed that the use of 100 mg l-1 NS solution after sterilization of the surface resulted in the highest percentage (89%) of disinfected explants. Nano silver solution did not affect the measured characters. According to the data obtained in the experience, it was concluded that NS had a good view to eliminate bacterial diseases in plant tissue culture procedures. Further research on other cultural species requires that in the tissue cultures of other cultures, to enhance the effectiveness of the NS in order to eliminate bacterial diseases.

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“…Using NS seems more convenient and less toxic than using antibiotics in the medium. Moreover, other methods for controlling the infection like microbial culture filtrate [15], and acidification of the medium [16], are time consuming. Satisfactory antimicrobial activity obtained in this research is consistent with the previous results in the literature [17,8].…”

Section: Resultsmentioning

confidence: 99%

Plant In vitro Culture goes Nano: Nanosilver-Mediated Decontamination of Ex vitro Explants

Mahna¹,

Vahed²,

Khani³

2013

J Nanomedic Nanotechnol

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Plant tissue culture is a basic and fundamental component of plant biotechnology, and progress in different fields of biotechnology greatly depends on the improvement of this technique. Nowadays, nanomaterials especially Nanosilver [NS], are regularly being used as an antimicrobial agent in medical and environmental fields. In order to assay the efficiency of NS in sterilizing plant seeds and fragile tissues, such as leaf and cotyledon, two important model plants, Arabidopsis and tomato, as well as potato as an important cultivated crop, were used as explants in this study. Briefly, samples soaked in different concentrations of NS with various exposure times, and then transferred onto the MS medium. Results showed that at lower concentrations, NS could function as an antimicrobial agent with no side effect on the explant viability, and consequently, all decontaminated seeds germinated, and leaf and cotyledon explants survived.

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A preliminary evaluation of the use of microbial culture filtrates for the control of contaminants in plant tissue culture systems

Cited by 13 publications

References 16 publications

Nano silver: a novel nanomaterial for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture

Nano silver: a novel nanomaterial for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture

Effect of Agno3 Nanoparticles on Sterilization and Alkaloid Production of Catherenthus Roseus Tissue By

Plant In vitro Culture goes Nano: Nanosilver-Mediated Decontamination of Ex vitro Explants

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