A preliminary study of mesenchymal stem cell-like cells derived from murine corneal stroma

Lü, Jin-Chun; Zhou, Ziyu; Zhang, Xiao-Rong; Li, Xiaolei; Wang, Hui‐Fang; Song, Xiujun

doi:10.1007/s00417-010-1367-0

Cited by 9 publications

(6 citation statements)

References 25 publications

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“…In mice, MSC-like cells isolated from the central part of the corneal stroma exhibited similar phenotype except for a higher CD34 and CD45 positivity, with the canonical differentiation potential being shown there as well 32 . Due to the limited MSC markers studied in mice, a full comparison and correspondence to our results are not feasible 33 . These findings, however, strengthen the previous hypothesis, that stem cells exist not just in the limbal epithelial crypts, but independently, in the central part of the cornea as well 33 34 35 36 37 38 39 40 .…”

Section: Discussionmentioning

confidence: 81%

Role of Human Corneal Stroma-Derived Mesenchymal-Like Stem Cells in Corneal Immunity and Wound Healing

Veréb

Póliska

Albert

et al. 2016

Sci Rep

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Corneal tissue regeneration is of crucial importance for maintaining normal vision. We aimed to isolate and cultivate human corneal stroma-derived mesenchymal stem-like cells (CSMSCs) from the central part of cadaver corneas and study their phenotype, multipotency, role in immunity and wound healing. The isolated cells grew as monolayers in vitro, expressed mesenchymal- and stemness-related surface markers (CD73, CD90, CD105, CD140b), and were negative for hematopoietic markers as determined by flow cytometry. CSMSCs were able to differentiate in vitro into fat, bone and cartilage. Their gene expression profile was closer to bone marrow-derived MSCs (BMMSCs) than to limbal epithelial stem cells (LESC) as determined by high-throughput screening. The immunosuppressive properties of CSMSCs were confirmed by a mixed lymphocyte reaction (MLR), while they could inhibit proliferation of activated immune cells. Treatment of CSMSCs by pro-inflammatory cytokines and toll-like receptor ligands significantly increased the secreted interleukin-6 (IL-6), interleukin-8 (IL-8) and C-X-C motif chemokine 10 (CXCL-10) levels, as well as the cell surface adhesion molecules. CSMSCs were capable of closing a wound in vitro under different stimuli. These cells thus contribute to corneal tissue homeostasis and play an immunomodulatory and regenerative role with possible implications in future cell therapies for treating sight-threatening corneal diseases.

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Section: Discussionmentioning

confidence: 81%

Role of Human Corneal Stroma-Derived Mesenchymal-Like Stem Cells in Corneal Immunity and Wound Healing

Veréb

Póliska

Albert

et al. 2016

Sci Rep

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“…In healthy human [107] and mouse corneas [104] a modest proportion of CD45 + resident cells express the hematopoietic stem cell marker CD34. Flow cytometric analysis of 70 pooled mouse corneas by Forrester and colleagues revealed that approximately 3% of all stromal cells express CD34 [56], a number that corroborates closely with the percentage of CD45 + cells reported in immunomorphological studies of the normal mouse cornea [104]. Considering that the majority of studies of human and mouse corneal macrophages agree that most CD45 + cells in healthy corneas phenotypically resemble macrophages [6,104,120,15,43], it is possible that these macrophages have a multipotent capacity to de-differentiate into keratocytes and contribute to collagen synthesis following injury, as has been demonstrated in vitro using primary cultures of isolated corneal stromal cells [107].…”

Section: Role In Homeostasismentioning

confidence: 99%

Macrophage physiology in the eye

Chinnery

McMenamin

Dando

2017

Pflugers Arch - Eur J Physiol

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The eye is a complex sensory organ composed of a range of tissue types including epithelia, connective tissue, smooth muscle, vascular and neural tissue. While some components of the eye require a high level of transparency to allow light to pass through unobstructed, other tissues are characterized by their dense pigmentation, which functions to absorb light and thus control its passage through the ocular structures. Macrophages are present in all ocular tissues, from the cornea at the anterior surface through to the choroid/sclera at the posterior pole. This review will describe the current understanding of the distribution, phenotype, and physiological role of ocular macrophages, and provide a summary of evidence pertaining to their proposed role during pathological conditions.

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“…As such, cultivated epithelial autografts have become widely used as a standard treatment for repairing the ocular surface [3]. Exploring deeper, a number of groups have more recently identified corneal/limbal stromal cells with stem cell properties [4][5][6][7][8][9][10] and similar studies are also being pursued for the innermost cellular layer, the corneal endothelium [11]. Nevertheless, the limited availability and sensitive location of corneal tissue present significant challenges for autologous corneal stem cell therapies, particularly in cases of bilateral disease.…”

Section: Introductionmentioning

confidence: 99%

Concise Reviews: Can Mesenchymal Stromal Cells Differentiate into Corneal Cells? A Systematic Review of Published Data

et al. 2015

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The majority of stem cell therapies for corneal repair are based upon the use of progenitor cells isolated from corneal tissue, but a growing body of literature suggests a role for mesenchymal stromal cells (MSC) isolated from noncorneal tissues. While the mechanism of MSC action seems likely to involve their immuno-modulatory properties, claims have emerged of MSC transdifferentiation into corneal cells. Substantial differences in methodology and experimental outcomes, however, have prompted us to perform a systematic review of the published data. Key questions used in our analysis included: the choice of markers used to assess corneal cell phenotype, the techniques used to detect these markers, adequate reporting of controls, and tracking of MSC when studied in vivo. Our search of the literature revealed 28 papers published since 2006, with half appearing since 2012. MSC cultures established from bone marrow and adipose tissue have been best studied (22 papers). Critically, only 11 studies used appropriate markers of corneal cell phenotype, along with necessary controls. Ten out of these eleven papers, however, contained positive evidence of corneal cell marker expression by MSC. The clearest evidence is observed with respect to expression of markers for corneal stromal cells by MSC. In comparison, the evidence for MSC conversion into either corneal epithelial cells or corneal endothelial cells is often inconsistent or inconclusive. Our analysis clarifies this emerging body of literature and provides guidance for future studies of MSC differentiation within the cornea as well as other tissues. STEM CELLS 2015;33:785-791

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A preliminary study of mesenchymal stem cell-like cells derived from murine corneal stroma

Cited by 9 publications

References 25 publications

Role of Human Corneal Stroma-Derived Mesenchymal-Like Stem Cells in Corneal Immunity and Wound Healing

Role of Human Corneal Stroma-Derived Mesenchymal-Like Stem Cells in Corneal Immunity and Wound Healing

Macrophage physiology in the eye

Concise Reviews: Can Mesenchymal Stromal Cells Differentiate into Corneal Cells? A Systematic Review of Published Data

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