A pressure-based bioassay for the rapid, portable and quantitative detection of C-reactive protein

Ji, Tao; Liu, Dan; Liu, Fang; Li, Jiuxing; Ruan, Qingyu; Song, Yanling; Tian, Tian; Zhu, Zhi; Zhou, Lili; Lin, Hui; Yang, Chaoyong; Wang, Dong

doi:10.1039/c6cc03705d

Cited by 55 publications

(32 citation statements)

References 25 publications

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“…Methods based on different signal-generation mechanisms have been utilized for the detection of antibiotic residues including fluorescence, surface-enhanced Raman spectroscopy, electrochemical method, and colorimetric assay . Inspiringly, the emergence of pressure-based bioassay opens a new horizon for the development of sensing systems by integrating the biomolecular recognition with a catalyzed gas-generation reaction into a portable device. − One major merit of using pressure-based bioassay is that one can control and tailor the system’s properties in a predictable manner to meet the requirements of specific applications without external cumbersome instrumentations. Shi et al creatively developed a simple point-of-care testing assay for the detection of microRNA on the basis of the change in gas pressure.…”

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confidence: 99%

Platinum Nanozyme-Catalyzed Gas Generation for Pressure-Based Bioassay Using Polyaniline Nanowires-Functionalized Graphene Oxide Framework

et al. 2018

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Pressure-based bioassays incorporating biomolecular recognition with a catalyzed gas-generation reaction have been developed for gas biosensors, but most involve poor sensitivity and are unsuitable for routine use. Herein we design an innovative gas pressure-based biosensing platform for the detection of Kanamycin (Kana) on polyaniline nanowires-functionalized reduced graphene oxide (PANI/rGO) framework by using platinum nanozyme-catalyzed gas generation. The signal was amplified by coupling with catalytic hairpin assembly (CHA) and strand-displacement amplification (SDA). Upon target Kana introduction, the analyte initially triggered a SDA reaction between hairpin DNA1 and hairpin DNA2, and then induced CHA conjugation between magnetic bead-labeled hairpin DNA3 (MB-H3) and platinum nanoparticle-labeled hairpin DNA4 (Pt-H4) to form a three-dimensional network. Numerous platinum nanoparticles (peroxidase-like nanozymes) were carried over with magnetic beads to reduce hydrogen peroxide into oxygen. The as-produced gas compressed PANI/rGO frameworks (modified to polyurethane sponge, used as the piezoelectric materials) in a homemade pressure-tight device, thus causing the increasing current of PANI/rGO sponge thanks to its deformation. The change in the current caused by the as-generated gas pressure was determined on an electrochemical workstation. Under optimum conditions, PANI/rGO sponge exhibited outstanding compressibility, stable signal-waveform output, fast response and recovery time (≈109 ms), and the current increased with the increasing Kana concentration within a dynamic working range of 0.2-50 pM at a detection limit of 0.063 pM. Good reproducibility, specificity, and acceptable precision were acquired for Kana analysis. In addition, the accuracy of this method was monitored to evaluate real milk samples with the well-matched results obtained by using the referenced Kana ELISA kit.

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confidence: 99%

Platinum Nanozyme-Catalyzed Gas Generation for Pressure-Based Bioassay Using Polyaniline Nanowires-Functionalized Graphene Oxide Framework

et al. 2018

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“…In order to evaluate the interference effect from the mixed sample (Hb+TRF+HSA+CRP) with the anti-CRP functionalized LSPR sensor, selective detection, and cross-reactivity to CRP were performed (Figure 8; Ji et al, 2016; Wu et al, 2016a). Our LSPR sensor showed a slight decrease in absorbance of CRP from 0.1 μg/mL concentration of mixed sample solution (Hb+TRF+HSA+CRP) compared to CRP single control sample, but no specificity for other proteins.…”

Section: Resultsmentioning

confidence: 99%

Development of a Cuvette-Based LSPR Sensor Chip Using a Plasmonically Active Transparent Strip

Heo

Bajpai

et al. 2019

Front. Bioeng. Biotechnol.

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This research demonstrates the development of a transmission-mode localized surface plasmon resonance (LSPR) sensor chip using a cuvette cell system for the sensitive detection of a biomolecule marker such as C-reactive protein (CRP). In order to develop a highly sensitive LSPR sensor chip, plasmonically active gold nanoparticles (AuNPs) were decorated onto various transparent substrates in the form of a uniform, high-density single layer using a self-assembly process. The transparent substrate surface was modified with amine functional groups via (3-Aminopropyl)triethoxysilane (APTES) treatment, and the ligand concentration and temperature (0.5% APTES at 60°C) were then optimized to control the binding energy with AuNPs. The optimized plasmonically active strip was subsequently prepared by dipping the amine-functionalized substrate into AuNPs for 8 h. The optimized plasmonic strip functionalized with anti-CRP was transformed into a portable LSPR sensor chip by placing it inside a cuvette cell system, and its detection performance was evaluated using CRP as a model sample. The detection limit for CRP using our LSPR sensor chip was 0.01 μg/mL, and the detection dynamic range was 0.01–10 μg/mL with a %CV of <10%, thus confirming its selectivity and good reproducibility. These findings illustrate that the highly sensitive portable LSPR biosensor developed in this study is expected to be widely used in a diverse range of fields such as diagnosis, medical care, environmental monitoring, and food quality control.

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“…There has been a recent trend to speed up this process, known as repurposing, based on expanding the capability of commercially available handheld devices to detect targets other than those intended. Thus, for example, simple devices used for monitoring physical or chemical parameters, such as pressure meters, thermometers, and pH meters, have been combined with biological recognition reactions enabling the determination of various types of clinical biomarkers [ 2 , 3 , 4 , 5 ]. Likewise, POC products already designed for the detection of biomarkers could be adapted for broadening the planned targets to other biomolecules of interest.…”

Section: Introductionmentioning

confidence: 99%

New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

Abardía-Serrano

Miranda‐Castro

de‐los‐Santos‐Álvarez

et al. 2020

Sensors

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A personal glucose meter (PGM)-based method for quantitative detection of a urinary nucleic acid biomarker in prostate cancer screening, the so-called PCA3, is reported herein. A sandwich-type genoassay is conducted on magnetic beads to collect the target from the sample by specific hybridization, making the assay appropriate for PCA3 detection in biological fluids. The success of the method hinges on the use of alkaline phosphatase (ALP) to link the amount of nucleic acid biomarker to the generation of glucose. In particular, specifically attached ALP molecules hydrolyze D-glucose-1-phosphate into D-glucose, thus enabling the amplification of the recorded signal on the personal glucose meter. The developed genoassay exhibits good sensitivity (3.3 ± 0.2 mg glucose dL−1 pM−1) for PCA3, with a dynamic range of 5 to 100 pM and a quantification limit of 5 pM. Likewise, it facilitates point-of-care testing of nucleic acid biomarkers by using off-the-shelf PGM instead of complex instrumentation involved in traditional laboratory-based tests.

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A pressure-based bioassay for the rapid, portable and quantitative detection of C-reactive protein

Cited by 55 publications

References 25 publications

Platinum Nanozyme-Catalyzed Gas Generation for Pressure-Based Bioassay Using Polyaniline Nanowires-Functionalized Graphene Oxide Framework

Platinum Nanozyme-Catalyzed Gas Generation for Pressure-Based Bioassay Using Polyaniline Nanowires-Functionalized Graphene Oxide Framework

Development of a Cuvette-Based LSPR Sensor Chip Using a Plasmonically Active Transparent Strip

New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

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