Development of a Cuvette-Based LSPR Sensor Chip Using a Plasmonically Active Transparent Strip

Abstract

This research demonstrates the development of a transmission-mode localized surface plasmon resonance (LSPR) sensor chip using a cuvette cell system for the sensitive detection of a biomolecule marker such as C-reactive protein (CRP). In order to develop a highly sensitive LSPR sensor chip, plasmonically active gold nanoparticles (AuNPs) were decorated onto various transparent substrates in the form of a uniform, high-density single layer using a self-assembly process. The transparent substrate surface was modified with amine functional groups via (3-Aminopropyl)triethoxysilane (APTES) treatment, and the ligand concentration and temperature (0.5% APTES at 60°C) were then optimized to control the binding energy with AuNPs. The optimized plasmonically active strip was subsequently prepared by dipping the amine-functionalized substrate into AuNPs for 8 h. The optimized plasmonic strip functionalized with anti-CRP was transformed into a portable LSPR sensor chip by placing it inside a cuvette cell system, and its detection performance was evaluated using CRP as a model sample. The detection limit for CRP using our LSPR sensor chip was 0.01 μg/mL, and the detection dynamic range was 0.01–10 μg/mL with a %CV of <10%, thus confirming its selectivity and good reproducibility. These findings illustrate that the highly sensitive portable LSPR biosensor developed in this study is expected to be widely used in a diverse range of fields such as diagnosis, medical care, environmental monitoring, and food quality control.

CRP Detection Using Gold Nanosensors – Effect of Layer Thickness and Immobilization Chemistry

“…This points to the fact that a saturation of the binding capacity is reached, even with the low concentration. A closer look on 13 and 19 reveals that the initial part of the curve is steeper in the case of higher concentration (13) compared to the lower one (19). When the secondary antibody is ushed in again ( 21), the same signal increase results as before in 15, pointing to good reproducibility.…”

“…The direct binding of CRP on anti-CRP antibodies (anti-CRP-AB)-modi ed gold nanospheres 19 , nanorods modi ed with single chain variable fragment (scFv) 20 , or silver nanoprisms modi ed with cytidine 5´diphosphocholine (PC) 21 allows this measurement principle. In combination with straightforward optical readout units, LSPR sensors can also open up a new eld of application for on-site diagnostics.…”

Biosensing Applications Using Nanostructure-Based Localized Surface Plasmon Resonance Sensors

“…Most flat substrate-based LSPR sensors use glass as a flat substrate on which to deposit AuNPs [ 31 , 57 , 59 , 60 , 61 , 62 , 63 , 64 ]. One of the simplest ways to fabricate such LSPR sensing substrates is to dip glass coated with 0.5% 3-aminopropyltriethoxysilane (APTES) as an amine group linker into an AuNP solution ([ 57 ]; Figure 2 A).…”

“…This sensor achieved a 10 4 CFU/mL limit of detection within 30–35 min. They further optimized the production conditions for AuNP-deposited glass substrates [ 60 ]. Specifically, the authors investigated the effect of glass thickness and quality on the properties of the final product.…”

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

“…CRP was used a model system to demonstrate the working of gold nanoparticles in a cuvette based localized surface plasmon resonance (LSPR) sensor chip also. The system was able to detect CRP but the linear range (0.01–10 μg/mL) and LOD of 0.01 μg/mL were not much impressive ( Oh et al, 2019 ). Park et al developed a fluorescent fullerene nanoparticle based sensor for the lateral flow immuno chromatographic assay of CRP.…”