A primordial dopamine D1‐like adenylyl cyclase‐linked receptor from Drosophila melanogaster displaying poor affinity for benzazepines

Abstract

Sequences reported in this paper have been deposited in GenBank with Accession Number U22106.Abbreviations: (-+)-6,7-ADTN, 6,7-dihyroxy-2-aminotetralin; CY 208 243, (-)-4,6,6a,7,8, 12b-hexahydro-7-methyl-indolo[4,3-ab]-phenanthridine; DA, dopamine; DHA, dihydroalprenolol; 8-OH-DPAT, (+)-8-hydroxy-N, N-dipropyl-2-aminotetralin; 5-HT, serotonin (5-hydroxytryptamine); L-dopa, L-3,4-dihydroxphenylalanine; LSD, lysergic acid diethylamine; NE, norepinephrine; N(-)-NPA, N-propyl-norapomorphine; SCH-23390, (R)-(+) -7-chloro-8-hydroxy-3-methyl-l-phenyl-2,3,4,5-tetrahydro-lH-3-benzazepine; SKF-38393, 2,3,4,5-tetrahydro-7,8-dihydroxy-l-phenyl-lH-3-benzazepine; SKF-81927, 6-chloro-7,8-dihydroxy-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine; SKF-82526, 6-chloro-7,8-dihydroxy-1 -(p-hydroxyphenyl)-2,3,4,5-tetrahydro-(1H)-3-benzazepine; TM, transmembrane; WB-4101, 2-(2,6-dimethoxyphenoxyethyl)aminomethyl-l,4-benzodioxane; YM-09151-2, eis-N-(1-benzyl-2-methylpyrrolidin-3-yl)-5-chloro-2-methoxy-4-methylamino-benzamide. cific motifs or amino acid residues confering high affinity benzazepine receptor interactions.

Evolution and expression of D2 and D3 dopamine receptor genes in zebrafish

“…The importance of dopaminergic signaling in evolution is reflected by the fact that dopamine modulates movement and behavior in species as diverse as worms and man, and dopamine receptors have been identified in a variety of invertebrate and vertebrate species. In Drosophila, cDNAs encoding D1-like (Gotzes et al, 1994;Sugamori et al, 1995;Feng et al, 1996;Han et al, 1996) and D2-like (Hearn et al, 2002) dopamine receptors have been characterized. Although the Drosophila polypeptides are considerably divergent in sequence from their mammalian counterparts, they exhibit the signaling and pharmacologic properties characteristic of mammalian D1-and D2-like dopamine receptors (Gotzes et al, 1994;Sugamori et al, 1995;Feng et al, 1996;Han et al, 1996;Hearn et al, 2002).…”

“…In Drosophila, cDNAs encoding D1-like (Gotzes et al, 1994;Sugamori et al, 1995;Feng et al, 1996;Han et al, 1996) and D2-like (Hearn et al, 2002) dopamine receptors have been characterized. Although the Drosophila polypeptides are considerably divergent in sequence from their mammalian counterparts, they exhibit the signaling and pharmacologic properties characteristic of mammalian D1-and D2-like dopamine receptors (Gotzes et al, 1994;Sugamori et al, 1995;Feng et al, 1996;Han et al, 1996;Hearn et al, 2002). cDNAs encoding D1-and D2-like dopamine receptors have been identified recently in the nematode Caenorhabditis elegans (Suo et al, 2002(Suo et al, , 2003.…”

Analysis of two D1-like dopamine receptors from the honey bee Apis mellifera reveals agonist-independent activity

“…cally distinct from those found in vertebrates. This sugUsing the baculovirus Autographa californica nugestion was supported by the cloning and in vitro characcleopolyhedrovirus as an expression vector, we have terization of two dopamine receptors from Drosophila, expressed AmDOP1 and AmDOP2 in insect (Spodoptera DAMB / DopR99B [12,15] and Dmdop1/dDA1 [13,45].…”

“…For example, the of cAMP production are used as a functional assay to vertebrate D1 receptor antagonist, SCH 23390, does not examine the agonist and antagonist activities of selected exhibit high affinit binding, nor is it particularly effective dopamine receptor ligands. The results reveal that these at blocking cAMP stimulation via these Drosophila dopatwo receptors have similar, but distinct, pharmacological mine receptors [13,45]. Moreover, the agonist activity of profiles and that AmDOP1, like the D5 / D1B class of SKF 38393 at these receptors is low compared to vertevertebrate dopamine receptors, exhibits agonist-indepenbrate dopamine receptors [37,45].…”

“…The results reveal that these at blocking cAMP stimulation via these Drosophila dopatwo receptors have similar, but distinct, pharmacological mine receptors [13,45]. Moreover, the agonist activity of profiles and that AmDOP1, like the D5 / D1B class of SKF 38393 at these receptors is low compared to vertevertebrate dopamine receptors, exhibits agonist-indepenbrate dopamine receptors [37,45]. dent activity.…”