A Profile of Bioactive Compounds of Rumex vesicarius L.

Abstract

The present study was designed to investigate the bioactive compounds in extracts of Rumex vesicarius L. (Polygonaceae), a wild edible herb growing in Egypt. Ethyl acetate and n-butanol fractions of leaves of R. vesicarius were conducted by HPLC-PDA-MS/MS-ESI in the negative mode to analyze phenolics content. Results revealed the identification of 13 phenolic compounds: 8-C-glucosyl-apigenin, 8-C-glucosyl-luteolin, 6-C-hexosyl-quercetin, 3-O-rutinosyl-quercetin, 7-O-rhamno-hexosyl-diosmetin, 7-O-rhamno-acetylhexosyl-diosmetin, catechin, epicatechin, ferulohexoside, 6-C-glucosyl-naringenin, epicatechin gallate, 6-C-glucosyl-catechin, and epigallocatechin gallate. Quantification of the identified compounds revealed that 6-C-glucosyl-naringenin was the major compound. Also, qualitative and quantitative analysis of the hydro-ethanolic extract of leaves was carried out for ascorbic acid, α-tocopherol, β-carotene. The essential oil as well as lipids analysis of saponifiable and unsaponifiable matters. The biochemical studies were conducted to evaluate the antioxidant and hepatoprotective potential of roots (REE), leaves (LEE), and fruits (FEE) ethanolic extracts of R. vesicarius (100 mg/kg b.wt., p.o., each) against hepatotoxicity induced by CCl(4) (0.5 mL/kg b.wt., p.o., 3 times a week) compared with silymarin (50 mg/kg b.wt., p.o.) as standard drug. The results confirmed that coadministration of the tested extracts or silymarin with CCl(4) for 4 wk exhibited a marked hepatoprotective activity, attributed to their antioxidant potential, membrane stabilizing effect, and antifibrogenic activities. Practical Application: Investigation of the effect of hommad as a safe hepatoprotective diet that prospectively directs the attention to a valuable therapeutic natural herb rich in bioactive constituents.

DETERMINATION OF FLAVONOIDS IN STAMEN, GYNOECIUM, AND PETALS OFMagnolia grandifloraL. AND THEIR ASSOCIATED ANTIOXIDANT AND HEPATOPROTECTION ACTIVITIES

“…They were characterized on the bases of their product ion spectra, comparisons with reference samples, and literature data. 17 − . The MS 2 product-ion analysis of the parent ion provided a fragmentation pattern of isorhamnetin glucoside, similar to the results previously reported in the literature.…”

Stability-indicating densitometric high-performance thin-layer chromatographic method for the quantitative analysis of biomarker naringin in the leaves and stems ofRumex vesicariusL.

“…R. vesicarius is reported to contain phytoconstituents like anthraquinones and flavonoids [3] and phenolic compounds [4]. Out of various phenolic constituents identified and analyzed in R. vesicarius, 6-Cglucosyl-naringenin was found to be the major phenolic compound [4].…”

“…R. vesicarius is reported to contain phytoconstituents like anthraquinones and flavonoids [3] and phenolic compounds [4]. Out of various phenolic constituents identified and analyzed in R. vesicarius, 6-Cglucosyl-naringenin was found to be the major phenolic compound [4]. It is experimentally proved that naringin can be converted to its various analogues, viz., naringenin 7-O-glucoside, naringenin, naringenin 4´,7-O-diglucoside, naringenin 5,7-Odiglucoside, naringenin 7-O-gentiobioside, naringenin 7-O-rutinoside, and 7-O-gentiobiosyl-4´-O-glucosylnaringenin [5].…”

Pharmacological screening of Polygonaceae species and isolation of biologically active compounds from Rumex aquaticus L. and Rumex thyrsiflorus Fingerh.

“…The major hydrocarbons were docosane, nonacosane and dodecane. 44 From the roots of R. acetosa, a polysaccharide was isolated. Structure determination indicated a polymer with molecular weight in the region of 300 000, consisting of D-glucose in high and D-arabinose in low proportion.…”

“…42 Qualitative and quantitative analysis of the hydro-ethanolic extract of R. vesicarius leaves demonstrated the presence of ascorbic acid and α-tocopherol. 44 From the roots of R. patientia, orcinol, a phenolic compound was isolated. 27 Its glucoside was isolated from the roots of R. nepalensis.…”