A proof-of-concept fluorescence lifetime camera based on a novel gated image sensor for fluorescence-guided surgery

Ingelberts, Hans; Lapauw, Thomas; Debie, Pieterjan; Hernot, Sophie; Kuijk, Maarten

doi:10.1117/12.2507803

Cited by 6 publications

(10 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fluorescence lifetime is the average time the molecule stays in an excited energy state after absorption of a photon and is an independent parameter which can be measured additionally to the spectral properties of fluorescence. The fluorescence lifetime depends on a number of things which each can be exploited for imaging: The structure of the fluorescent molecule: imaging the lifetime can aid in revealing which fluorescent molecule is present or distinguish different fluorescent molecules [ 21 ]. The chemical environment of the fluorescent molecule: fluorescence lifetime has a sensitivity to chemical conditions such as pH or Ca+ or O 2 concentrations and can as such be used as a probe for these conditions [ 41 ].…”

Section: Cmos Photodetectors Using Current-assistancementioning

confidence: 99%

“…The structure of the fluorescent molecule: imaging the lifetime can aid in revealing which fluorescent molecule is present or distinguish different fluorescent molecules [ 21 ].…”

Section: Cmos Photodetectors Using Current-assistancementioning

confidence: 99%

“…A first-generation 32 × 32-pixel CAPS array has been realized in a standard 350 nm CMOS process on a high resistivity (~1000 Ω·cm), thick p-type epilayer (14 µm). A camera is developed around this to demonstrate the capabilities of the CAPS-array [ 21 ]. The pixels in the array measure 30 × 30 µm with an active area of 480 µm 2 resulting in a fill factor of 53%.…”

Section: Cmos Photodetectors Using Current-assistancementioning

confidence: 99%

“…Over the years, the prefix “current-assistance” has been found in an increasing number of different photonic sensors: current-assisted photonic demodulators (CAPD) for time-of-flight imaging [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ], fast time-gated current-assisted photonic samplers (CAPS) for high-precision fluorescence lifetime imaging [ 19 , 20 , 21 , 22 ], current-assisted photodetector (CAP) for optical receivers [ 23 ] and current-assisted single-photon avalanche diodes (CA-SPAD) for fast and efficient single-photon detection [ 24 , 25 ]. This review paper focusses on research performed at the Department of electronics and informatics (ETRO), Vrije Universiteit Brussel (VUB) using this “current-assistance” principle.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An Overview of CMOS Photodetectors Utilizing Current-Assistance for Swift and Efficient Photo-Carrier Detection

Jegannathan

Seliuchenko

Dries

et al. 2021

Sensors

Self Cite

View full text Add to dashboard Cite

This review paper presents an assortment of research on a family of photodetectors which use the same base mechanism, current assistance, for the operation. Current assistance is used to create a drift field in the semiconductor, more specifically silicon, in order to improve the bandwidth and the quantum efficiency. Based on the detector and application, the drift field can be static or modulated. Applications include 3D imaging (both direct and indirect time-of-flight), optical receivers and fluorescence lifetime imaging. This work discusses the current-assistance principle, the various photodetectors using this principle and a comparison is made with other state-of-the-art photodetectors used for the same application.

show abstract

Section: Cmos Photodetectors Using Current-assistancementioning

confidence: 99%

“…The structure of the fluorescent molecule: imaging the lifetime can aid in revealing which fluorescent molecule is present or distinguish different fluorescent molecules [ 21 ].…”

Section: Cmos Photodetectors Using Current-assistancementioning

confidence: 99%

Section: Cmos Photodetectors Using Current-assistancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An Overview of CMOS Photodetectors Utilizing Current-Assistance for Swift and Efficient Photo-Carrier Detection

Jegannathan

Seliuchenko

Dries

et al. 2021

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…Yet, each additional tap must share the number of photons available. More sensitive, fast gated cameras [37] could be used for better exploitation of the photon budget. Furthermore, time-of-flight imaging is a flourishing field, currently boosted by technological trends like autonomous driving [38].…”

Section: Discussionmentioning

confidence: 99%

Towards real-time wide-field fluorescence lifetime imaging of 5-ALA labeled brain tumors with multi-tap CMOS cameras

Reichert

Erkkilä

Holst³

et al. 2020

Biomed. Opt. Express

View full text Add to dashboard Cite

Fluorescence guided neurosurgery based on 5-aminolevulinic acid (5-ALA) has significantly increased maximal safe resections. Fluorescence lifetime imaging (FLIM) of 5-ALA could further boost this development by its increased sensitivity. However, neurosurgeons require real-time visual feedback which was so far limited in dual-tap CMOS camera based FLIM. By optimizing the number of phase frames required for reconstruction, we here demonstrate real-time 5-ALA FLIM of human high-and low-grade glioma with up to 12 Hz imaging rate over a wide field of view (11.0 x 11.0 mm). Compared to conventional fluorescence imaging, real-time FLIM offers enhanced contrast of weakly fluorescent tissue.

show abstract

Fluorescence Guided Surgery

Stewart

Birch

2021

Methods Appl. Fluoresc.

View full text Add to dashboard Cite

Fluorescence guided surgery (FGS) is an imaging technique that allows the surgeon to visualise different structures and types of tissue during a surgical procedure that may not be as visible under white light conditions. Due to the many potential advantages of fluorescence guided surgery compared to more traditional clinical imaging techniques such as its higher contrast and sensitivity, less subjective use, and ease of instrument operation, the research interest in fluorescence guided surgery continues to grow over various key aspects such as fluorescent probe development and surgical system development as well as its potential clinical applications. This review looks to summarise some of the emerging opportunities and developments that have already been made in fluorescence guided surgery in recent years while highlighting its advantages as well as limitations that need to be overcome in order to utilise the full potential of fluorescence within the surgical environment.

show abstract

A proof-of-concept fluorescence lifetime camera based on a novel gated image sensor for fluorescence-guided surgery

Cited by 6 publications

References 10 publications

An Overview of CMOS Photodetectors Utilizing Current-Assistance for Swift and Efficient Photo-Carrier Detection

An Overview of CMOS Photodetectors Utilizing Current-Assistance for Swift and Efficient Photo-Carrier Detection

Towards real-time wide-field fluorescence lifetime imaging of 5-ALA labeled brain tumors with multi-tap CMOS cameras

Fluorescence Guided Surgery

Contact Info

Product

Resources

About