A Proposed Biochemical Basis for the Genus Pseudomonas

Studies on Certain Species of Bacteria Assigned to the Genus Chromobacterium

“…Although the transmittancy curves of C. iodinum and C. viscosum, figure 3, are not completely similar, there is a relationship shown in respect to the general location of transmittancy peaks in the ultraviolet region. This shows that each has a transmittancy peak in the ultraviolet at approximately 300 mp& and an absorption peak in the ultraviolet at approximately 350 m;p. This relationship is strengthened further by the fact that P. aeruginosa produces a pigment known to be a phenazine derivative (Tobie, 1945), and that phenazine derivatives have this distinctive peak of absorption in the ultraviolet, figure 3. It is known further that the pigment of C. iodinum is a phenazine derivative (Tobie, 1945).…”

“…This shows that each has a transmittancy peak in the ultraviolet at approximately 300 mp& and an absorption peak in the ultraviolet at approximately 350 m;p. This relationship is strengthened further by the fact that P. aeruginosa produces a pigment known to be a phenazine derivative (Tobie, 1945), and that phenazine derivatives have this distinctive peak of absorption in the ultraviolet, figure 3. It is known further that the pigment of C. iodinum is a phenazine derivative (Tobie, 1945). Also, C. visco8um and P. aeruginosa show similar transmittancy peaks at 510 mu.…”

“…Cruess-Callaghan and Gorman (1935) published descriptions of C. violaceum and other violet bacteria, and Grimes (1927) described C. viscosum, and Davis (1939) described C. iodinum. Tobie (1945) suggested that the best classification of pigment producing bacteria would be one based on biochemistry. He suggested that all bacteria producing yellowish-green or brownishgreen fluorescent pigments, which were soluble in water or diffusible in media, or a pigment of a phenazine derivative regardless of color, or both, should be placed in the genus Pseudomonas. With the history of the classification of these species in mind, it was the objective of this study to describe more fully the relationships between the species in question and, by so doing, either enhance a more logical cla#sification of the doubtful species or substantiate their present classification.…”

Characterization of Certain Gram-Negative Bacteria from Surface Waters1

“…Of 336 cultures having an oxidative glucose metabolism which were isolated by this procedure, only 9 failed to produce fluorescent pigment on King's B medium, including all isolates which produced a surface alkaline reaction on Hugh and Leifson's medium. Gram-negative rod-shape, oxidative glucose metabolism, the production of fluorescent pigment, and polar flagellation would be sufficient to place these bacteria in the genus Pseudomonas, according to Tobie (1945) and Park (1962). Since flagella stains of all cultures isolated were not examined, no positive statement on classification can be made.…”

Siderophore mediated metal uptake by <i>Pseudomonas fluorescens</i> and its comparison to Iron (III) chelation

“…Pseudomonas fluorescens was grown for 3 days in BR medium and Nutrient broth which were suitable for intense pigment production. The culture free broth was extracted with equal volumes of isopropanol and chloroform (Tobie, 1945) and the resultant solvent fractions was allowed to evaporate under refrigeration. The extract was redissolved in 5ml of distilled water and used as a crude siderophore preparation for TLC as well as for metal complexation studies.…”