A proposed lectin‐mediated mechanism to explain the in Vivo antihyperglycemic activity of γ‐conglutin from <i>Lupinus albus</i> seeds

Grácio, Madalena; Rocha, José Antonio; Pinto, Rui; Ferreira, Ricardo B.; Solas, J; Figueira, Maria Eduardo; Sepodes, Bruno; Ribeiro, Ana C.

doi:10.1002/fsn3.2520

Cited by 7 publications

(7 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The serum levels of creatinine and urea in all treated groups presented lower levels than in the control group. However, these lower results were not interpreted as an indication of toxicity since the control group (which received only water) also showed lower values compared to historical control values (0.28–0.37 mg/dL) [ 28 , 29 ]. The observation of decreased values of creatinine led us to discard the presence of toxicity since only increased creatinine levels are considered indicative of kidney injury [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

Malú

Lima

Malmir

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

Dried leaves of Lannea velutina A. Rich. and Sorindeia juglandifolia (A. Rich.) Planch. ex Oliv. (family Anacardiaceae) are used in African traditional medicine. Although these medicinal plants have widespread use in the treatment of inflammatory diseases, there is no scientific data concerning their preclinical or clinical safety. This work aimed to investigate the phytochemical properties of the leaves of both species using HPLC-UV/DAD, as well as the in vivo oral repeated-dose toxicity of 70% hydroethanolic leaf extract of S. juglandifolia and the in vitro genotoxicity of 70% hydroethanolic leaf extracts of L. velutina and S. juglandifolia. Clinical signs of toxicity, body weight variations, and changes in food consumption, mortality, and blood biochemical parameters were monitored. Genotoxicity was assessed using the bacterial reverse mutation assay (Ames test) with and without metabolic activation, according to OECD guidelines. The obtained results showed the presence of gallic acid and anacardic acid as the main marker constituents in both species. No significant changes in general body weight or food intake were observed; small significant changes with no critical relevance were observed in the blood biochemistry of animals treated with S. juglandifolia hydroethanolic extract (50, 400, and 1000 mg/kg body weight) compared to those in the control group. No genotoxicity was observed in the bacterial reverse mutation assay with S. juglandifolia and L. velutina extracts (up to 5 mg/plate). The safety data obtained in vivo and lack of genotoxic potential in vitro points to the safe medicinal use of S. juglandifolia and L. velutina extracts.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The Ames test was carried out following OECD guideline No. 471 [ 24 ] and the ICH S2 (R1) guideline [ 29 ], according to previously published protocols [ 37 , 46 ].…”

Section: Methodsmentioning

confidence: 99%

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

Malú

Lima

Malmir

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…Only the protein from soy beverages, with the exception of low amounts of cysteine and methionine, was comparable to cow’s milk when nutritional composition and health benefits are discussed among commercial vegetable alternatives ( 50 ). Several advantages for human health are attributed to chickpea proteins and lupin proteins: the lupin lectin γ-conglutin, a globulin composed of 42 kDa subunits, showed beneficial properties through its reducing effect in glycemia being suitable for diabetics ( 14 , 51 ); the deflamin protein found in legume seeds, namely lupin and chickpea, has been shown to be anti-inflammatory in in vitro models with various colon cancer cell lines as well as in vivo models of acute and chronic disease, presenting inhibitory bioactivity against MMP-9 ( 24 , 27 ). Also, the seed reserve protein β-conglutin from Lupinus albus exerted action on glycemic modulation and reduced the circulating cholesterol ( 15 ), and a natural 210 kDa glyco-oligomer fungicide, termed Blad-containing oligomer present in Lupinus albus , showed potent antifungal activity against both human and phytopathogenic fungi ( 52 ).…”

Section: Discussionmentioning

confidence: 99%

“…The technique used to remove offflavors from legume-based beverages is thermal inactivation, but high temperatures lead to denaturation, aggregation, reduction in protein solubility, and nutrient losses (e.g., vitamins and minerals), which have restricted its use as a promising technology. In addition, some bioactive compounds from legume seeds, such as protease inhibitors (PIs), seed reserve proteins (γ-conglutin), lectins, phytates, oligosaccharides, saponins, and phenolic compounds may have important metabolic effects on the consumer's health (11)(12)(13)(14)(15). Some of these substances are beneficial, while others are considered anti-nutritional factors, which overall impair digestion, such as PIs, which decrease the digestibility and absorption of proteins (16).…”

Section: Introductionmentioning

confidence: 99%

New Alternatives to Milk From Pulses: Chickpea and Lupin Beverages With Improved Digestibility and Potential Bioactivities for Human Health

et al. 2022

Self Cite

View full text Add to dashboard Cite

There is a strong demand for plant-based milk substitutes, often low in protein content (<1.5% w/v). Protein-rich pulse seeds and the right processing technologies make it possible to make relevant choices. The major objective of this study was to assess the impact of processing on the nutritional characteristics of beverages with a high impact on health, in particular on digestibility and specific bioactivities. The results suggest that pulse beverages are as high in protein content (3.24% w/v for chickpea and 4.05% w/v for lupin) as cow’s milk. The anti-nutrient level characteristics of pulses have been considerably reduced by strategic processing. However, when present in small quantities, some of these anti-nutritional factors may have health benefits. Controlling processing conditions play a crucial role in this fine balance as a tool to take advantage of their health benefits. There is evidence of protein hydrolysis by in vitro digestion and limited bioaccessibility of minerals. In addition to being highly digestible, lupin and chickpea beverages have anti-inflammatory and anti-carcinogenic potential evaluated through the inhibition of metalloproteinase MMP-9.

show abstract

“…Duranti et al (1995) reported a L. albus -conglutin with lectin-like activity and the ability to bind glycoproteins. Recently, an hemagglutinating -conglutin from L. albus was isolated (Grácio et al, 2021), but its activity was not constant in different purified batches, indicating that the methodology was not ideal. The hemagglutinating activity (Schoeneberger et al, 1982;Djabayan-Djibeyan et al, 2022) and the affinity for galactose of seed extracts from Lupinus mutabilis was reported (Falcon, 2000b), but the purification protocols were not suitable and caused the loss of the hemagglutinating capacity (Falcón et al, 2000b).…”

Section: Introductionmentioning

confidence: 99%

Isolation of a Hemagglutinating γ-conglutin from Seeds of the Andean Legume Lupinus mutabilis Sweet (Tarwi)

Carnero¹,

Alzamora-Gonzales²,

Colona-Vallejos³

et al. 2023

View full text Add to dashboard Cite

Background: Lupinus mutabilis Sweet is a species of Andean legume with a high content of proteins, which present hemagglutinating lectins. Here, we demonstrated that the γ-conglutin lectin isolated from L. mutabilis seeds is responsible for the hemagglutinating activity by evaluating this activity at each step of the isolation process. Methods: Seeds of three ecotypes of L. mutabilis were used. Saline protein extraction size exclusion and ionic exchange chromatography were performed to isolate the lectin. Carbohydrate specificity, thermostability and resistance to chelating and reducing agents of the lectin were tested. SDS-page and mass spectrometry were performed to characterize the isolated hemagglutinating lectin. Rabbit erythrocyte hemagglutination test was performed at each step. Result: Patón Grande ecotype had higher hemagglutinating titers and therefore was selected for further purification steps. Hemagglutinating activity of the purified lectin, which was identified as a γ-conglutin, was cation-independent and optimal between 15-20°C. Besides, it resisted temperatures up to 70°C, its activity was lost in basic pH and remained active under reducing and chelating conditions.

show abstract

A proposed lectin‐mediated mechanism to explain the in Vivo antihyperglycemic activity of γ‐conglutin from Lupinus albus seeds

Cited by 7 publications

References 44 publications

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

New Alternatives to Milk From Pulses: Chickpea and Lupin Beverages With Improved Digestibility and Potential Bioactivities for Human Health

Isolation of a Hemagglutinating γ-conglutin from Seeds of the Andean Legume Lupinus mutabilis Sweet (Tarwi)

Contact Info

Product

Resources

About