A proposed nomenclature for biological processes that remove nitrogen

Weißbach, M.; Criddle, Craig S.; Drewes, Jörg E.; Koch, Konrad

doi:10.1039/c6ew00216a

Cited by 16 publications

(13 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nitrate/nitrite reduction via NO dismutation can be referred to as oxygenic denitrification to facilitate discussion. It can also be referred to as a potential new oxygenic route in nitrous denitritation, in line with the nomenclature proposed for biological nitrogen removal systems (13). It is interesting to consider whether the putative nod genes detected in the different systems could be affiliated with microbes other than "Ca.…”

Section: Discussionmentioning

confidence: 99%

“…The two reactors of the CANDO system, one performing partial nitrification to nitrite and one nitrous denitritation to N 2 O, were designated CANDO-nitritation and CANDO-N 2 O, respectively (Table 2). A comprehensive redefinition of the nomenclature for biological processes contributing to nitrogen removal in such engineered water systems can be found elsewhere (13).…”

Section: Methodsmentioning

confidence: 99%

“…For example, such populations can be hypothesized to occur in contaminated aquifers or in wastewater treatment systems. Wastewater treatment systems especially offer a wealth of distinct niches for microbes involved in biological nitrogen removal (13).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Unexpected Diversity and High Abundance of Putative Nitric Oxide Dismutase (Nod) Genes in Contaminated Aquifers and Wastewater Treatment Systems

Zhu

Bradford

Huang

et al. 2017

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

It has recently been suggested that oxygenic dismutation of NO into N 2 and O 2 may occur in the anaerobic methanotrophic "Candidatus Methylomirabilis oxyfera" and the alkane-oxidizing gammaproteobacterium HdN1. It may represent a new pathway in microbial nitrogen cycling catalyzed by a putative NO dismutase (Nod). The formed O 2 enables microbes to employ aerobic catabolic pathways in anoxic habitats, suggesting an ecophysiological niche space of substantial appeal for bioremediation and water treatment. However, it is still unknown whether this physiology is limited to "Ca. Methylomirabilis oxyfera" and HdN1 and whether it can be coupled to the oxidation of electron donors other than alkanes. Here, we report insights into an unexpected diversity and remarkable abundance of nod genes in natural and engineered water systems. Phylogenetically diverse nod genes were recovered from a range of contaminated aquifers and N-removing wastewater treatment systems. Together with nod genes from "Ca. Methylomirabilis oxyfera" and HdN1, the novel environmental nod sequences formed no fewer than 6 well-supported phylogenetic clusters, clearly distinct from canonical NO reductase (quinoldependent NO reductase [qNor] and cytochrome c-dependent NO reductase [cNor]) genes. The abundance of nod genes in the investigated samples ranged from 1.6 ϫ 10 7 to 5.2 ϫ 10 10 copies · g Ϫ1 (wet weight) of sediment or sludge biomass, accounting for up to 10% of total bacterial 16S rRNA gene counts. In essence, NO dismutation could be a much more widespread physiology than currently perceived. Understanding the controls of this emergent microbial capacity could offer new routes for nitrogen elimination or pollutant remediation in natural and engineered water systems.IMPORTANCE NO dismutation into N 2 and O 2 is a novel process catalyzed by putative NO dismutase (Nod). To date, only two bacteria, the anaerobic methaneoxidizing bacterium "Ca. Methylomirabilis oxyfera" and the alkane-oxidizing gammaproteobacterium HdN1, are known to harbor nod genes. In this study, we report efficient molecular tools that can detect and quantify a wide diversity of nod genes in environmental samples. A surprisingly high diversity and abundance of nod genes were found in contaminated aquifers as well as wastewater treatment systems. This evidence indicates that NO dismutation may be a much more widespread physiology in natural and man-made environments than currently perceived. The molecular tools presented here will facilitate further studies on these enigmatic microbes in the future.KEYWORDS nitric oxide (NO) dismutation, NO dismutase, oxygenic denitrification,

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Unexpected Diversity and High Abundance of Putative Nitric Oxide Dismutase (Nod) Genes in Contaminated Aquifers and Wastewater Treatment Systems

Zhu

Bradford

Huang

et al. 2017

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, these factors amongst others were also reported to influence the production of dissolved nitrous oxide (N 2 O) potentially being emitted into the atmosphere due to diffusion and stripping induced by aeration (Kampschreur et al, 2009b). Different N 2 O production pathways have been reported, including nitrifier denitrification (or also termed 'aerobic nitrous denitritation' as proposed by Weißbach et al (2017)) and hydroxylamine oxidation by AOBs as well as incomplete heterotrophic denitrification due to inhibition of N 2 O reductase (Ding et al, 2016;Law et al, 2012; Rathnayake et al, 2015). In contrast, the metabolic pathway of AnAOBs is not known to be capable of N 2 O production (Kartal et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Strategies for enhanced deammonification performance and reduced nitrous oxide emissions

Leix

Drewes

et al. 2017

Bioresource Technology

Self Cite

View full text Add to dashboard Cite

Deammonification's performance and associated nitrous oxide emissions (NO) depend on operational conditions. While studies have investigated factors for high performances and low emissions separately, this study investigated optimizing deammonification performance while simultaneously reducing NO emissions. Using a design of experiment (DoE) method, two models were developed for the prediction of the nitrogen removal rate and NO emissions during single-stage deammonification considering three operational factors (i.e., pH value, feeding and aeration strategy). The emission factor varied between 0.7±0.5% and 4.1±1.2% at different DoE-conditions. The nitrogen removal rate was predicted to be maximized at settings of pH 7.46, intermittent feeding and aeration. Conversely, emissions were predicted to be minimized at the design edges at pH 7.80, single feeding, and continuous aeration. Results suggested a weak positive correlation between the nitrogen removal rate and NO emissions, thus, a single optimizing operational set-point for maximized performance and minimized emissions did not exist.

show abstract

“…Es gibt mehrere Hinweise darauf, dass Distickstoffoxid (N 2 O, dinitrogen oxide bzw. nitrous oxide ) bei der Abwasserbehandlung , im Bereich von 0,4 bis 25 % proportional zur Stickstoffbelastung entstehen kann . Verschiedene Prozessbedingungen sind für diese Emissionen verantwortlich, die während biochemischer Reaktionen entstehen können.…”

Section: Introductionunclassified

N₂O‐Vermeidung bei der Behandlung hoch stickstoffreicher Abwässer

Lindenblatt

Drewes

2019

Chemie Ingenieur Technik

Self Cite

View full text Add to dashboard Cite

Bezogen auf den Stickstoffabbau zeigen die N2O‐Emissionen große Unterschiede bei der zweistufigen Behandlung mit Nitritation als erster Stufe gegenüber der einstufigen Deammonifikation. Es sind Messergebnisse der Nitritation im Kontaktprozess mit kontinuierlicher Beschickung und Nachklärung sowie der Behandlung im Sequencing‐Batch‐Reaktor gegenübergestellt. Während der biologischen Zentratbehandlung wurden N2O‐Konzentrationen in Reaktoren und der Abluft gemessen. Bei ähnlicher NH4‐N‐Abbauleistung liegen die N2O‐Emissionen in der einstufigen Deammonifikation um eine Zehnerpotenz niedriger.

show abstract

A proposed nomenclature for biological processes that remove nitrogen

Abstract: A consistent terminology is proposed to resolve present inconsistencies and to facilitate distinct communication about biological nitrogen removal processes.

Cited by 16 publications

References 72 publications

Unexpected Diversity and High Abundance of Putative Nitric Oxide Dismutase (Nod) Genes in Contaminated Aquifers and Wastewater Treatment Systems

Unexpected Diversity and High Abundance of Putative Nitric Oxide Dismutase (Nod) Genes in Contaminated Aquifers and Wastewater Treatment Systems

Strategies for enhanced deammonification performance and reduced nitrous oxide emissions

N₂O‐Vermeidung bei der Behandlung hoch stickstoffreicher Abwässer

Contact Info

Product

Resources

About

A proposed nomenclature for biological processes that remove nitrogen

Abstract: A consistent terminology is proposed to resolve present inconsistencies and to facilitate distinct communication about biological nitrogen removal processes.

Cited by 16 publications

References 72 publications

Unexpected Diversity and High Abundance of Putative Nitric Oxide Dismutase (Nod) Genes in Contaminated Aquifers and Wastewater Treatment Systems

Unexpected Diversity and High Abundance of Putative Nitric Oxide Dismutase (Nod) Genes in Contaminated Aquifers and Wastewater Treatment Systems

Strategies for enhanced deammonification performance and reduced nitrous oxide emissions

N2O‐Vermeidung bei der Behandlung hoch stickstoffreicher Abwässer

Contact Info

Product

Resources

About

N₂O‐Vermeidung bei der Behandlung hoch stickstoffreicher Abwässer