A prospective study characterizing full-length hepatitis B virus genomes during acute exacerbation

Liu, Chun‐Jen; Chen, Pei‐Jer; Lai, Ming‐Yang; Kao, Jia‐Horng; Chang, Ching-Fung; Wu, Hui‐Lin; Shau, Wen–Yi; Chen, Ding‐Shinn

doi:10.1053/gast.2003.50003

Cited by 63 publications

(54 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pathogenesis of HBV infection involves repeated necroinflammation and regeneration, as well as the interplay among the virus, host immunity, and hepatocytes [2]. Therefore, both host and viral factors are known to contribute to the clinical outcomes of chronic hepatitis B [3].…”

Section: Introductionmentioning

confidence: 99%

High serum adiponectin correlates with advanced liver disease in patients with chronic hepatitis B virus infection

et al. 2008

Self Cite

View full text Add to dashboard Cite

Purpose Adiponectin possesses anti-inflammatory and insulin-sensitizing properties. Little is known about the role of adiponectin in hepatitis B-related liver disease.Methods Serum adiponectin and hepatitis B viral factors were cross-sectionally assayed in 280 patients with chronic hepatitis B virus (HBV) infection including 120 patients with chronic HBV infection, 40 patients with cirrhosis, and 120 patients with hepatocellular carcinoma (HCC); 116 healthy adults were used as controls. The dynamics of serum adiponectin level was also studied longitudinally in 25 patients with hepatitis B e antigen (HBeAg) seroconversion (SC). Results We found that serum adiponectin level in patients with chronic HBV infection was similar to that in healthy controls and was significantly lower than patients with cirrhosis and HCC. In univariate analysis, high serum adiponectin level significantly correlated with the presence of HBV-related cirrhosis or HCC, abnormal serum ALT level, and HBV genotype C. Multivariate analysis revealed that high serum adiponectin level significantly correlated with the development of HCC. Serum adiponectin levels remained stationary in patients experiencing HBeAg SC. Conclusions Our findings suggest that HBV infection itself does not affect adiponectin levels. Serum adiponectin level correlates with the progression of HBV-related liver diseases but not with the development of HBeAg SC.

show abstract

Section: Introductionmentioning

confidence: 99%

High serum adiponectin correlates with advanced liver disease in patients with chronic hepatitis B virus infection

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…Serum DNA was extracted and collected as previously described. 6,7 Liver DNA was extracted from 10 to 25 mg liver biopsy tissue specimens stored in liquid nitrogen using a commercially available Abbreviations: HBeAg, hepatitis B e antigen; B, baseline; ALT-S, serum ALT; AP, after exacerbation; ALT-P, peak of serum ALT; DNA-S, serum HBV DNA; DNA-P, peak of serum HBV DNA during exacerbation; M, male; Ba, Asia subtype of HBV genotype B; F, female; C, HBV genotype C; ϩ, positive; Ϫ, negative.…”

Section: Virological Assaysmentioning

confidence: 99%

“…We then performed polymerase chain reaction (PCR) and sequencing of the full-length genome of the dominant HBV strain, as previously described. 6,7,13 Preparation and Sequencing of HBV Covalently Closed Circular DNA (cccDNA) From Liver Biopsy Specimens. From the life-cycle point of view, all serum and intrahepatic HBVs originate from the cccDNA within the nucleus of the infected hepatocyte.…”

Section: Virological Assaysmentioning

confidence: 99%

“…In addition to direct sequencing, the subcloning and sequencing of full-length or subgenomic PCR products obtained from serum, liver specimens and intrahepatic cccDNA were also performed in randomly selected cases as previously described. 6,7,13 Determination of the Nucleotide and Derived Amino Acid Changes of the Dominant HBV Strains in Patients With Consecutive Episodes of AE. The nucleotides and their derived amino acid sequences of the dominant HBV strains obtained at different time-points in each AE were compared with the corresponding one at enrollment (baseline of first AE) for each patient.…”

Section: Virological Assaysmentioning

confidence: 99%

“…2 Hepatitis B virus (HBV) evolves rapidly in chronic hepatitis B patients, and the correlation of HBV variation with the onset of AE has been extensively studied. [3][4][5] Previously, we investigated sequential full-length viral sequences in patients with AE 6,7 and found that most exacerbations were preceded by an upsurge of serum HBV identical to the preexisting HBV strain. After exacerbation, however, about half of the patients were repopulated by a different viral variant in the circulation.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Origin of serum hepatitis B virus in acute exacerbation: Comparison with HBV in the liver and from other exacerbation

et al. 2004

Self Cite

View full text Add to dashboard Cite

Acute exacerbation (AE) of chronic hepatitis B is usually preceded by reemergence or increase of hepatitis B virus (HBV) in the serum. To investigate the origin of the reemergence or increase, we compared the identity of the serum viral genome to that in the liver and in previous AE by full-length sequencing. The full-length viral genome and extent of quasispecies were obtained from serum and liver biopsy specimens at the same time from 9 subjects with hepatitis B exacerbation (group I). Composition of viral quasispecies was compared by the genetic diversity and the average number of nucleotide substitutions within and between different viral sources. Another 2 patients with repeated AEs (group II) were also enrolled, and their serial serum alanine aminotransferase, HBV DNA levels and full-length sequences were determined. In all group I patients, serum viral genome was identical to that in the liver. The genetic diversity and the average number of nucleotide difference were also comparable between serum and liver tissue. In 2 group II patients, the viral variant that emerged after previous AE was not identical to that caused by the subsequent AE. Dominant viral strains for serial AEs in a single patient did not show a sequential evolution, but presented as a horizontal selection of a minor population from the original viral pool. In conclusion, the findings suggest that viral strain in serum reflects the intrahepatic strain of the AE. Random reactivation of the original HBV pool, rather than a sequential evolution of one strain, also contributes to the onset of repeated AE. A cute exacerbation (AE) occurs frequently in hepatitis B surface antigen carriers. 1 The exacerbation can accelerate the progression of liver disease. 2 Hepatitis B virus (HBV) evolves rapidly in chronic hepatitis B patients, and the correlation of HBV variation with the onset of AE has been extensively studied. [3][4][5] Previously, we investigated sequential full-length viral sequences in patients with AE 6,7 and found that most exacerbations were preceded by an upsurge of serum HBV identical to the preexisting HBV strain. After exacerbation, however, about half of the patients were repopulated by a different viral variant in the circulation.The pathogenesis of AE actually takes place in the liver. Therefore, it is better to directly characterize the viral genome from the liver biopsy specimen. As noted previously, some potential pathogenic strains may replicate actively within the liver but not be released into the circulation. 8 Thus, HBV genomic changes that are correlated with the development of hepatitis B exacerbation might occur and be retained within the liver but not be detected in the specimens obtained from the circulation. To resolve these questions, the representation of the serum viral genome to the corresponding intrahepatic one was first determined by comparing the dominant viral nucleotide sequence and genetic complexity of the viral quasispecies between both tissue specimens obtained si-

show abstract

Hepatitis after arterial embolization for hepatocellular carcinoma

Huo

Lee

2006

Hepatology

View full text Add to dashboard Cite

A prospective study characterizing full-length hepatitis B virus genomes during acute exacerbation

Cited by 63 publications

References 40 publications

High serum adiponectin correlates with advanced liver disease in patients with chronic hepatitis B virus infection

High serum adiponectin correlates with advanced liver disease in patients with chronic hepatitis B virus infection

Origin of serum hepatitis B virus in acute exacerbation: Comparison with HBV in the liver and from other exacerbation

Hepatitis after arterial embolization for hepatocellular carcinoma

Contact Info

Product

Resources

About