A prospective study of leucocyte mitochondrial DNA content and deletion in association with the metabolic syndrome

Kim, J.-Y.; Choi, Jong Rak; Park, I.H.; Huh, Ji Hye; Son, Jung Woo; Kim, K.W.; Park, K.-S.; Kuy, Seung; Sohn, Joon Hyung; Jung, Dong-Hyuk; Koh, Sang Baek

doi:10.1016/j.diabet.2016.09.007

Cited by 7 publications

(6 citation statements)

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“…[30][31][32][33] Given the robust evidence demonstrating an association between a low mtDNAcn in peripheral blood and cardiovascular and metabolic diseases, one could speculate that low levels of this biomarker reflect an unfavorable metabolic pattern predisposing to the development of this PE subtype. [21][22][23][24][25] A possible alternative or complementary explanation is suggested by the chorionic villi immaturity that characterizes the placental histology of PE associated with AGAf. In this regard, the role of mitochondria in energy production is critically important during embryo development and for placental function.…”

Section: Discussionmentioning

confidence: 99%

“…However, several authors observed a reduced peripheral blood mtDNA content in patients with conditions traditionally characterized by an excessive oxidative stress such as metabolic syndrome and coronary heart disease. [21][22][23][24][25] On these basis, we hypothesized a possible correlation between mtDNAcn in peripheral blood in the first trimester of pregnancy and the onset of PE associated with early placental damage and fetal growth restriction. The aim of this study was thus to explore whether mtDNAcn in maternal peripheral blood is reduced in pregnancies that develop PE associated with fetal growth restriction.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mitochondrial DNA Copy Number in Peripheral Blood in the First Trimester of Pregnancy and Different Preeclampsia Clinical Phenotypes Development: A Pilot Study

et al. 2018

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Inflammation and oxidative stress are intrinsically linked to early poor placentation, typical of pregnancies complicated by preeclampsia associated with intrauterine growth restriction (PE-IUGR). Low mitochondrial DNA copy number (mtDNAcn) in peripheral blood constitutes a good peripheral surrogate marker of inflammation and oxidative stress. On these basis, we explored a possible correlation between mtDNAcn in peripheral blood in the first trimester of pregnancy and the PE-IUGR onset. To shed light on this issue, we setup a nested case–control study from a prospective cohort of pregnant women undergoing first-trimester aneuploidies screening. Two groups of patients affected by PE classified according to the clinical phenotype were identified: (1) patients who developed PE-IUGR and (2) patients who developed PE associated with appropriate for gestational age intrauterine fetal growth (PE-AGAf). Controls were women with a physiologic pregnancy matched to cases on the basis of age (±6 months, ratio 2:1). Mitochondrial DNA copy number was quantified using real-time polymerase chain reaction and normalized to nuclear DNA. The median (interquartile range) mtDNAcn in peripheral blood in patients with PE-IUGR (n = 12) and in patients with PE-AGAf (n = 16) was 70 (44-97) and 108 (95-145), respectively ( P = .004). Both these values were significantly lower than that detected in the control group (161[133-183], P < .001). The area under the receiver–operator curve for PE-IUGR and PE-AGAf were 0.94 (95% confidence interval [CI]: 0.88-1.00, P < .001) and 0.81 (95%CI: 0.70-0.91, P < .001), respectively. In conclusion, MtDNAcn in peripheral blood resulted significantly lower both in patients affected by PE-IUGR and in those affected by PE-AGAf when compared to controls. The accuracy of this biomarker resulted particularly good in predicting PE-IUGR.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mitochondrial DNA Copy Number in Peripheral Blood in the First Trimester of Pregnancy and Different Preeclampsia Clinical Phenotypes Development: A Pilot Study

et al. 2018

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“…mtDNA content can be an indicator of mitochondrial (dys)function 23 . A lower mtDNA copy number is a potential biomarker for type 2 diabetes mellitus 24 , while an increased mtDNA copy number is linked with a lower risk of metabolic syndrome in adults 25 . Furthermore, mtDNA content has been positively associated with metabolic hormones such as leptin and insulin in early life 26,27 .…”

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confidence: 99%

Breastfeeding predicts blood mitochondrial DNA content in adolescents

Cosemans

Nawrot

Janssen

et al. 2020

Sci Rep

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nutrition during early childhood is linked to metabolic programming. We hypothesized that breastfeeding has long-term consequences on the energy metabolism exemplified by mitochondrial DnA (mtDnA). As part of the third cycle of the flemish environment and Health Study (fLeHSiii) cohort, 303 adolescents aged 14-15 years were included. We associated breastfeeding and blood mtDNA content 14-15 years later while adjusting for confounding variables. Compared with nonbreastfed adolescents, mtDNA content was 23.1% (95%CI: 4.4-45.2; p = 0.013) higher in breastfed adolescents. Being breastfed for 1-10 weeks, 11-20 weeks, and >20 weeks, was associated with a higher mtDNA content of respectively 16.0% (95%CI: −7.1-44.9; p = 0.191), 23.5% (95%CI: 0.8-51.3; p = 0.042), and 31.5% (95%CI: 4.3-65.7; p = 0.021). Our study showed a positive association between breastfeeding and mtDnA content in adolescents which gradually increased with longer periods of breastfeeding. Higher mtDNA content may be an underlying mechanism of the beneficial effects of breastfeeding on children's metabolism. Exclusive breastfeeding (i.e. not formula-feeding) for six months is recommended by the World Health Organization (WHO) for optimal growth, development, and health of the child 1. Breast milk is known to have many benefits for the child's health, like strengthening their immune system and it has a positive effect on brain development and cognitive function. Studies on the effect of breastfeeding stated a decline in infection rates in childhood 2 , an improvement in white matter development 3 , a better psychomotor development during the first year of life 4 , and higher cognitive development scores 5. Breastfeeding may, in addition, be associated with a lower risk of type 2 diabetes mellitus 6 and has a protective effect against obesity 7. The potential mechanisms through which breastfeeding can have positive effects on children's development may be due to the composition of breast milk (i.e. presence of nutrients and absence of preservatives), the optimal supply of breast milk, or the improved bond between mothers and their child 8. However, the exact cellular and molecular mechanisms through which breastfeeding exerts its positive effects are still unknown. Mitochondria are intracellular organelles responsible for energy production by producing adenosine triphosphate (ATP), a substrate required for metabolism. Every cell contains various mitochondria, each with multiple copies of mitochondrial DNA (mtDNA) 9. Decreased mitochondrial function can cause impaired cellular functions and give rise to a variety of human diseases such as cardiovascular diseases 10 , cancer 11 , diabetes mellitus and metabolic syndrome 12,13 , autoimmune diseases 14,15 , and neurodegenerative and-behavioral diseases 16-18. In addition, placental mtDNA content was positively associated with neurocognition in children 19. Impaired mitochondrial function also plays a role in obesity-related 7 and cardiovascular diseases 20. Mitochondrial function can be altered by environme...

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“…Recently, Kim et al reported that reduced leukocyte mitochondrial DNA (mtDNA) copy number and increased mtDNA deletion ratios are independent predictors of new-onset metabolic syndrome (MetS) in a population-based longitudinal study [1]. Authors suggest that the risk of MetS development could be estimated by measuring mtDNA copy number and deletion ratio, baseline of which was measured with a quantitative polymerase chain reaction (qPCR) in leukocytes.…”

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confidence: 99%

Advanced Mitochondrial DNA Assay for Metabolic Syndrome

Sohn

2016

J Lifestyle Med

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A prospective study of leucocyte mitochondrial DNA content and deletion in association with the metabolic syndrome

Cited by 7 publications

References 10 publications

Mitochondrial DNA Copy Number in Peripheral Blood in the First Trimester of Pregnancy and Different Preeclampsia Clinical Phenotypes Development: A Pilot Study

Mitochondrial DNA Copy Number in Peripheral Blood in the First Trimester of Pregnancy and Different Preeclampsia Clinical Phenotypes Development: A Pilot Study

Breastfeeding predicts blood mitochondrial DNA content in adolescents

Advanced Mitochondrial DNA Assay for Metabolic Syndrome

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