A protease-free and signal-on electrochemical biosensor for ultrasensitive detection of lead ion based on GR-5 DNAzyme and catalytic hairpin assembly

Huang, Xinyi; Li, Junlong; Zhang, Qiongyuan; Chen, Shuai; Xu, Wei; Wu, Jiayi; Niu, Wuceng; Xue, Jianjiang; Li, Chaorui

doi:10.1016/j.jelechem.2018.03.036

Cited by 21 publications

(5 citation statements)

References 56 publications

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“…On the basis of catalytic hairpin assembly, a wide range of sensors has been developed over the past few years. With the use of similar principles as the HCR sensors discussed above, the CHA reaction has been applied to the detection of the same analyte classes (i.e., metal ions, − DNA and DNA mismatches, − mRNA, microRNAs, − ,− proteins, ,− and cells) . As for the HCR, analytes other than nucleic acids can be detected by coupling the CHA scheme to aptamer target recognition.…”

Section: Applications In Sensing Diagnostics and Therapeuticsmentioning

confidence: 99%

Principles and Applications of Nucleic Acid Strand Displacement Reactions

2019

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Dynamic DNA nanotechnology, a subfield of DNA nanotechnology, is concerned with the study and application of nucleic acid strand-displacement reactions. Strand-displacement reactions generally proceed by three-way or four-way branch migration and initially were investigated for their relevance to genetic recombination. Through the use of toeholds, which are single-stranded segments of DNA to which an invader strand can bind to initiate branch migration, the rate with which strand displacement reactions proceed can be varied by more than 6 orders of magnitude. In addition, the use of toeholds enables the construction of enzyme-free DNA reaction networks exhibiting complex dynamical behavior. A demonstration of this was provided in the year 2000, in which strand displacement reactions were employed to drive a DNAbased nanomachine et al. Nature 2000, 406, 605−608). Since then, toeholdmediated strand displacement reactions have been used with ever increasing sophistication and the field of dynamic DNA nanotechnology has grown exponentially. Besides molecular machines, the field has produced enzyme-free catalytic systems, all DNA chemical oscillators and the most complex molecular computers yet devised. Enzyme-free catalytic systems can function as chemical amplifiers and as such have received considerable attention for sensing and detection applications in chemistry and medical diagnostics. Strand-displacement reactions have been combined with other enzymatically driven processes and have also been employed within living cells (Groves, B.; et al. Nat. Nanotechnol. 2015, 11, 287−294). Strand-displacement principles have also been applied in synthetic biology to enable artificial gene regulation and computation in bacteria. Given the enormous progress of dynamic DNA nanotechnology over the past years, the field now seems poised for practical application.

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Section: Applications In Sensing Diagnostics and Therapeuticsmentioning

confidence: 99%

Principles and Applications of Nucleic Acid Strand Displacement Reactions

2019

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“…Table shows the performance parameters of the reported electrochemical sensors, such as the linear range, limit of detection and detection time. Compared to other sensors , the detecting parameters of Pbzyme/SWNTs/FET were beyond most electrochemical sensors, except DNA/SWNTs/FET. DNA/SWNTs/FET for Pb 2+ determination had a wide linear rang and lower limit of detection of were much better, but the detection time reached 20 min, which was not suitable to rapid detection in site.…”

Section: Resultsmentioning

confidence: 99%

“…SWNTs modified single‐gap microelectrode (SWNTs/FETs) are highly sensitive to low concentrations of analyte . Therefore, a sensitive and selective biosensor using SWNTs and GR‐5 DNAzyme was proposed, which were modified on the single‐gap microelectrode (Pbzyme/SWNTs/FET) for the determination of Pb 2+ . The mechanism utilizes the number of carriers in SWNTs affected significantly by the biomaterials attached to the SWNTs’ surface.…”

Section: Introductionmentioning

confidence: 93%

Single‐gap Microelectrode Functionalized with Single‐walled Carbon Nanotubes and Pbzyme for the Determination of Pb²⁺

2019

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Lead is a highly toxic metal, which can persist in the natural environment and enrich in biological bodies. It can cause many severe diseases in the human body even at extremely low concentration. Here, we developed a new biosensor using single‐walled carbon nanotubes (SWNTs) modified with a specific Pbzyme (Pbzyme/SWNTs/FET) to detect lead ion (Pb2+), which can monitor the lead pollution. This biosensor used 3‐aminopropyltriethoxysilane to immobilize SWNTs on the area between the source and the drain of single‐gap microelectrode (FET), and the duplex DNA (Pbzyme) consisted of DNAzyme (GR‐5) and complementary DNA (CS‐DNA) was functionalized with the SWNTs’ surface through a peptide bond. The use of GR‐5 DANzyme and Pb2+ to form a stable complex structure to cleave the CS‐DNA can change radically the Pbzyme's structure on the SWNTs’ surface, which will further affect the number of carriers in SNWTs and the conductivity of the Pbzyme/SWNTs/FET. The change in conductivity can be used to evaluate the Pb2+ concentration. Under the optimal conditions, the relative resistances presented a positive correlation with the Pb2+ concentrations, showing a good linear relationship in the range of 10 pM to 50 nM, where the linear regression equation was y=10.104log [CPb]+5.8656, and the detection limit was 7.4 pM. Finally, the biosensor was applied to measure the Pb2+ contents in the soil collected from the forest grass green belt and paint, and the results were compared with the results of atomic fluorescence spectrometry.

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“…Besides, the Agzyme/SWNTs-FET exhibited a significantly high recovery response that ranged from 92.45% to 105.12% which implied the proposed sensor can quantify the Ag + ions in real river water with acceptable accuracy. Wang and colleagues also demonstrated new strategies for electrical detection of Pb 2+ concerning the potential of GR-5 DNAzyme (Pbzyme) [ 121 ] which could cleave the substrate strand at the RNA site in the presence of Pb 2+ [ 122 , 123 ]. The sensor structure was similar to the previously mentioned sensor for detection of Ag + ( Figure 6 a).…”

Section: Carbon-based Fetsmentioning

confidence: 99%

Ten Years Progress of Electrical Detection of Heavy Metal Ions (HMIs) Using Various Field-Effect Transistor (FET) Nanosensors: A Review

et al. 2021

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Heavy metal pollution remains a major concern for the public today, in line with the growing population and global industrialization. Heavy metal ion (HMI) is a threat to human and environmental safety, even at low concentrations, thus rapid and continuous HMI monitoring is essential. Among the sensors available for HMI detection, the field-effect transistor (FET) sensor demonstrates promising potential for fast and real-time detection. The aim of this review is to provide a condensed overview of the contribution of certain semiconductor substrates in the development of chemical and biosensor FETs for HMI detection in the past decade. A brief introduction of the FET sensor along with its construction and configuration is presented in the first part of this review. Subsequently, the FET sensor deployment issue and FET intrinsic limitation screening effect are also discussed, and the solutions to overcome these shortcomings are summarized. Later, we summarize the strategies for HMIs’ electrical detection, mechanisms, and sensing performance on nanomaterial semiconductor FET transducers, including silicon, carbon nanotubes, graphene, AlGaN/GaN, transition metal dichalcogenides (TMD), black phosphorus, organic and inorganic semiconductor. Finally, concerns and suggestions regarding detection in the real samples using FET sensors are highlighted in the conclusion.

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A protease-free and signal-on electrochemical biosensor for ultrasensitive detection of lead ion based on GR-5 DNAzyme and catalytic hairpin assembly

Cited by 21 publications

References 56 publications

Principles and Applications of Nucleic Acid Strand Displacement Reactions

Principles and Applications of Nucleic Acid Strand Displacement Reactions

Single‐gap Microelectrode Functionalized with Single‐walled Carbon Nanotubes and Pbzyme for the Determination of Pb²⁺

Ten Years Progress of Electrical Detection of Heavy Metal Ions (HMIs) Using Various Field-Effect Transistor (FET) Nanosensors: A Review

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A protease-free and signal-on electrochemical biosensor for ultrasensitive detection of lead ion based on GR-5 DNAzyme and catalytic hairpin assembly

Cited by 21 publications

References 56 publications

Principles and Applications of Nucleic Acid Strand Displacement Reactions

Principles and Applications of Nucleic Acid Strand Displacement Reactions

Single‐gap Microelectrode Functionalized with Single‐walled Carbon Nanotubes and Pbzyme for the Determination of Pb2+

Ten Years Progress of Electrical Detection of Heavy Metal Ions (HMIs) Using Various Field-Effect Transistor (FET) Nanosensors: A Review

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Single‐gap Microelectrode Functionalized with Single‐walled Carbon Nanotubes and Pbzyme for the Determination of Pb²⁺