A protein isolated from human oviductal tissue in vitro secretion, identified as human lactoferrin, interacts with spermatozoa and oocytes and modulates gamete interaction

Zumoffen, Carlos; Gil, Roberto R.; Caille, Adriana; Morente, Carlos; Munuce, María José; Ghersevich, Sergio

doi:10.1093/humrep/det016

Cited by 42 publications

(64 citation statements)

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“…It was detected in tubal fluid and appeared to be estrogen regulated in human oviduct epithelial cells (Zumoffen et al 2013). Results of our study indicated that lactoferrin presents different binding patterns to sperm related to the capacitation status and the acrosome reaction, suggesting that the protein could participate in sperm-oocyte interaction ( Fig.…”

Section: Lactoferrinmentioning

confidence: 62%

“…Results of our study indicated that lactoferrin presents different binding patterns to sperm related to the capacitation status and the acrosome reaction, suggesting that the protein could participate in sperm-oocyte interaction ( Fig. 3; Zumoffen et al 2013). In addition, lactoferrin was able to modulate some sperm functions related to capacitation (C Zumoffen and S Ghersevich unpublished observations).…”

Section: Lactoferrinmentioning

confidence: 80%

“…In addition, the presence of the protein caused a dosedependent decrease in the human sperm-zona interaction (Zumoffen et al 2013). Lactoferrin was also shown to reduce the availability of sperm D-mannose receptors (C Zumoffen and S Ghersevich, unpublished observations).…”

Section: Lactoferrinmentioning

confidence: 97%

“…This glycoprotein was isolated in our laboratory from the conditioned medium of human oviductal tissue culture based on its binding ability to sperm membrane and was further identified as human lactoferrin (Zumoffen et al 2013). It was detected in tubal fluid and appeared to be estrogen regulated in human oviduct epithelial cells (Zumoffen et al 2013).…”

Section: Lactoferrinmentioning

confidence: 99%

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Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

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Experimental evidence from the last 30 years supports the fact that the oviduct is involved in the modulation of the reproductive process in eutherian mammals. Oviductal secretion contains molecules that contribute to regulation of gamete function, gamete interaction, and the early stages of embryo development. The oviductal environment would act as a sperm reservoir, maintaining sperm viability, and modulating the subpopulation of spermatozoa that initiates the capacitation process. It could also contribute to prevent the premature acrosome reaction and to reduce polyspermy. Many studies have reported the beneficial effects of the oviductal environment on fertilization and on the first stages of embryo development. Some oviductal factors have been identified in different mammalian species. The effects of oviductal secretion on the reproductive process could be thought to result from the dynamic combined action (inhibitory or stimulatory) of multiple factors present in the oviductal lumen at different stages of the ovulatory cycle and in the presence of gametes or embryos. It could be hypothesized that the absence of a given molecule would not affect fertility as its action could be compensated by another factor with similar functions. However, any alteration in this balance could affect certain events of the reproductive process and could perhaps impair fertility. Thus, the complexity of the reproductive process warrants a continuous research effort to unveil the mechanisms and factors behind its regulation in the oviductal microenvironment.

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Section: Lactoferrinmentioning

confidence: 62%

Section: Lactoferrinmentioning

confidence: 80%

Section: Lactoferrinmentioning

confidence: 97%

Section: Lactoferrinmentioning

confidence: 99%

See 2 more Smart Citations

Oviductal secretion and gamete interaction

Ghersevich¹,

Massa²,

Zumoffen³

2015

Reproduction

View full text Add to dashboard Cite

show abstract

“…Lactoferrin, an oestrogen-regulated glycoprotein, was first identified in human OF. Depending on the sperm capacitation status, this protein exhibited different binding patterns, suggesting its involvement in gamete interaction (Zumoffen et al 2013). Oviductin, or oviduct-specific glycoprotein (OVGP1), is an oestrus-associated protein that has been demonstrated to be highly conserved in all species studied (Aviles et al 2010).…”

Section: Oviductal Fluidmentioning

confidence: 99%

Oviductal response to gametes and early embryos in mammals

Maillo¹,

Sánchez‐Calabuig²,

Lopera-Vásquez³

et al. 2016

Reproduction

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The oviduct is a complex and organized thin tubular structure connecting the ovary with the uterus. It is the site of final sperm capacitation, oocyte fertilization and, in most species, the first 3-4 days of early embryo development. The oviductal epithelium is made up of ciliary and secretory cells responsible for the secretion of proteins and other factors which contribute to the formation of the oviductal fluid. Despite significant research, most of the pathways and oviductal factors implicated in the crosstalk between gametes/early embryo and the oviduct remain unknown. Therefore, studying the oviductal environment is crucial to improve our understanding of the regulatory mechanisms controlling fertilization and embryo development. In vitro systems are a valuable tool to study in vivo pathways and mechanisms, particularly those in the oviducts which in livestock species are challenging to access. In studies of gamete and embryo interaction with the reproductive tract, oviductal epithelial cells, oviductal fluid and microvesicles co-cultured with gametes/embryos represent the most appropriate in vitro models to mimic the physiological conditions in vivo.Reproduction (2016) 152 R127-R141

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S100 A9 is expressed and secreted by the oviduct epithelium, interacts with gametes and affects parameters of human sperm capacitation in vitro

Massa

Prez

Zumoffen

et al. 2019

J of Cellular Biochemistry

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Our previous findings demonstrate that some oviductal secretion proteins bind to gametes and affect sperm physiology and gamete interaction. One of these proteins possesses an estimated molecular weight of 14 kDa. The objective of this study was to isolate and identify this 14 kDa protein, to localize it in the human oviduct, to detect gamete binding sites for the protein, and to evaluate its effects on sperm capacitation parameters and gamete interaction. Explants from the human oviductal tissues of premenopausal women were cultured in the presence of [35S]‐Methionine‐proteins ([35S]‐Met‐proteins). De novo synthesized secreted [35S]‐Met‐proteins were isolated from the culture media by affinity chromatography using their sperm membrane binding ability and analysed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Using liquid chromatography‐tandem mass spectrometry peptide sequencing, human S100 A9 was identified as one of the isolated proteins from the 14 kDa protein band. S100 A9 was detected in oviduct epithelium and oviduct secretion using immunohistochemistry and a Western blot. S100 A9 binding to human oocytes and spermatozoa was assessed by indirect immunofluorescence. The acrosome reaction (AR) affected S100 A9 ability to bind sperm cells. The presence of S100 A9 significantly increased both the induced AR and the sperm protein tyrosine phosphorylation, with respect to controls. However, the protein did not affect sperm‐zona pellucida interaction. Results indicate that S100 A9 is present in the human oviduct and that it modulates parameters of sperm capacitation in vitro. Hence, the protein might contribute to the regulation of the reproductive process in the oviductal microenvironment.

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A protein isolated from human oviductal tissue in vitro secretion, identified as human lactoferrin, interacts with spermatozoa and oocytes and modulates gamete interaction

Abstract: This study was supported by grants from FONCYT (PICT 01095, S.A.G., M.J.M) and SECyT UNR (PIDBIO238, S.A.G). The authors have no conflict of interest to declare.

Cited by 42 publications

References 50 publications

Oviductal secretion and gamete interaction

Oviductal secretion and gamete interaction

Oviductal response to gametes and early embryos in mammals

S100 A9 is expressed and secreted by the oviduct epithelium, interacts with gametes and affects parameters of human sperm capacitation in vitro

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