A proteome-based assessment of the earthworm Eisenia fetida: response to chemical warfare agents in a sandy loam soilThe 7th international symposium on earthworm ecology · Cardiff · Wales · 2002

Kuperman, Roman G.; Checkai, Ronald T.; Ruth, Lisa M.; Henry, Tammy; Simini, Michael; Kimmel, David G.; Phillips, Carlton T.; Bradley, Brian P.

doi:10.1016/s0031-4056(04)70245-0

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…Like in many other ecologically important species, proteomics research in earthworms lags far behind other model species, such as Mus musculus and Caenorhabditis elegans. There are only five research papers about proteomics of earthworm until now [11][12][13][14][15]. The key technologies of the proteome analysis are protein separation by 2-DE and mass spectrometric protein identification, which result in the efficient analysis of hundreds to thousands of proteins expressed in cells at one time [16].…”

Section: Introductionmentioning

confidence: 99%

A two-dimensional electrophoresis reference map of the earthworm Eisenia fetida

Wang¹,

Zhang²,

Sun³

2012

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The molecular mechanisms underlying innate immunity in the earthworm E. fetida remain unclear. For the recognition of innate immunity in the earthworm E. fetida, a detailed knowledge of this proteome is a prerequisite. The absence of a high-resolution E. fetida proteome map prompted us to determine E. fetida protein spots that can be visualised on 2-D protein gels. In this study, we present a preliminary description of the whole earthworm E. fetida proteome. A highly detailed two-dimensional gel electrophoresis (2-DE) map of the E. fetida proteome was established and approximately 1500 protein spots were detected from the earthworm sample when applying a 500 μg protein 2-DE in the pH range 3.0 - 10.0. We present a 2-DE proteome map of E. fetida, identifying 76 different proteins by matrix-assisted laser desorption/ionization-tandem time of flight mass spectrometry (MALDI-TOF/ TOF-MS) analysis. These identified proteins, including heat shock protein 90 (Hsp90), chaperonine protein HSP60, caspase-8, fibrinolytic protease 0, gelsolin-like protein, lombricine kinase, coelomic cytolytic factor1 (CCF 1), manganous superoxide dismutase (MnSOD), triosephosphate isomerase, extracellular globin-4, lysenin, and intermediate filament protein, glyceraldehyde-3- phosphate dehydrogenase, et al., are involved in several processes, including transcripttion, translation, the tricarboxylic acid cycle, the cellular amino acid metabolic process, protein amino acid phosphorylation, glycolysis, and the glucose metabolic process. These 2-DE data will enhance future comparisons of immunity, toxicology, biotic processes and other challenges, thereby allowing for further study of the molecular mechanisms in response to environmental stressors, and it will be useful to investigate environmental proteomics in invertebrate earthworms

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Section: Introductionmentioning

confidence: 99%

A two-dimensional electrophoresis reference map of the earthworm Eisenia fetida

Wang¹,

Zhang²,

Sun³

2012

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Analysis of earthworm Eisenia fetida proteomes during cadmium exposure: An ecotoxicoproteomics approach

et al. 2010

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Identification of differential proteomic responses to cadmium (Cd) would provide a means for better understanding the survival mechanisms of the earthworm, Eisenia fetida, living in a Cd-polluted environment, and the stress responses can be assessed by ecotoxiptoromics approaches. Extracts of whole earthworm collected at days 7, 14, 21, and 28 after Cd exposure were analyzed by 2-DE and quantitative image analysis. In total, 143 proteins demonstrated significant regulation in at least at one time point, and 56 proteins were identified by MALDI-TOF/TOF-MS and database searching. Compared with control samples, 28 protein spots were up-regulated and 28 were down-regulated during at least one time point. The identified proteins, including chaperonine protein HSP60, caspase-8, calcium ion-binding protein, zinc ion-binding protein, actin-binding protein, proteolysis, fibrinolytic protease, glutamate dehydrogenase, gelsolin-like protein, lombricine kinase, coelomic cytolytic factor 1, manganous superoxide dismutase, extracellular globin-4, lysenin, intermediate filament protein, and tubulin, are involved in several processes, including transcription, translation, the tricarboxylic acid cycle, the cellular amino acid metabolic process, protein amino acid phosphorylation, glycolysis, and the glucose metabolic process. Thus, our study provides a functional profile of the Cd-responsive proteins in earthworms.

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A summary of eleven years progress in earthworm ecotoxicologyThe 7th international symposium on earthworm ecology · Cardiff · Wales · 2002

2003

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A proteome-based assessment of the earthworm Eisenia fetida: response to chemical warfare agents in a sandy loam soilThe 7th international symposium on earthworm ecology · Cardiff · Wales · 2002

Cited by 4 publications

References 17 publications

A two-dimensional electrophoresis reference map of the earthworm <i>Eisenia fetida</i>

A two-dimensional electrophoresis reference map of the earthworm <i>Eisenia fetida</i>

Analysis of earthworm Eisenia fetida proteomes during cadmium exposure: An ecotoxicoproteomics approach

A summary of eleven years progress in earthworm ecotoxicologyThe 7th international symposium on earthworm ecology · Cardiff · Wales · 2002

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A proteome-based assessment of the earthworm Eisenia fetida: response to chemical warfare agents in a sandy loam soilThe 7th international symposium on earthworm ecology · Cardiff · Wales · 2002

Cited by 4 publications

References 17 publications

A two-dimensional electrophoresis reference map of the earthworm &lt;i&gt;Eisenia fetida&lt;/i&gt;

A two-dimensional electrophoresis reference map of the earthworm &lt;i&gt;Eisenia fetida&lt;/i&gt;

Analysis of earthworm Eisenia fetida proteomes during cadmium exposure: An ecotoxicoproteomics approach

A summary of eleven years progress in earthworm ecotoxicologyThe 7th international symposium on earthworm ecology · Cardiff · Wales · 2002

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A two-dimensional electrophoresis reference map of the earthworm <i>Eisenia fetida</i>

A two-dimensional electrophoresis reference map of the earthworm <i>Eisenia fetida</i>