A proteomic approach to study pea (Pisum sativum) responses to powdery mildew (Erysiphe pisi)

Curto, Miguel; Camafeita, Emilio; López, Juan Antonio; Maldonado, Antonio; Rubiales, Diego; Jorrı́n, Jesús

doi:10.1002/pmic.200500396

Cited by 95 publications

(64 citation statements)

References 77 publications

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“…Thus minor changes of protein expression in the resistant plant were observed. Similar evidence was also reported earlier in resistant cultivars of pea in response to powdery mildew fungus, Erysiphe pisi in comparison to the susceptible cultivars 24 . The combination of MALDI-TOF MS analysis and NCBI database searching allowed the identification of 9 protein spots.…”

Section: Discussionsupporting

confidence: 90%

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Wongpia¹,

Lomthaisong

2010

ScienceAsia

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Wilt disease caused by Fusarium oxysporum f. sp. capsici is a major problem of chilli pepper production worldwide that calls for a better understanding of defensive mechanisms in the chilli plant. We used a proteomic technique to investigate protein responses of chilli pepper to F. oxysporum f. sp. capsici. Two cultivars of resistant (Mae Ping 80) and susceptible (Long Chilli 455) plants were cultured in vitro. Chilli plants at 6-week growth were then infected with a suspension of F. oxysporum f. sp. capsici or distilled water used as a control. After 48 h of infection, proteins were extracted and analysed using 2DE to identify the responsive proteins. At least 9 spots were differentially expressed in the resistant cultivar (5 increasing, 4 decreasing) and 1 supplementary; while 15 increasing, 11 decreasing, and 11 supplementary protein spots were found in the susceptible cultivar. These proteins were then identified by MALDI-TOF MS combined with bioinformatics methods. Some of the induced proteins e.g., NADPH HC toxin reductase, serine/threonine protein kinase, and 1-aminocyclopropane-1-carboxylate synthase 3 are involved in plant defence mechanism. In order to determine the Fusarium wilt protective proteins in chilli plant, the protein patterns of healthy resistance were compared with those of susceptible cultivars. Interestingly, resistance showed higher expression of proteins related to ROS detoxification. Moreover, the ability of chilli plant to resist Fusarium wilt disease was related to the expression of non-inducible immunity 1 protein.

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Section: Discussionsupporting

confidence: 90%

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Wongpia¹,

Lomthaisong

2010

ScienceAsia

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“…Following CBB staining of the gels, an average of 350 spots was resolved, this figure being within the range observed for leaves of other plant species analyzed [13,18]. The 2-DE pattern was quite reproducible in the three replicates of the same treatment, and at least 250 spots were commonly represented in all of them (genotype or treatment) (Figs.…”

Section: Discussionmentioning

confidence: 79%

“…Proteins were extracted by using the TCA-acetone precipitation protocol and quantified with the RCDC protein assay (Bio-Rad) as described [13].…”

Section: Sample Preparationmentioning

confidence: 99%

Proteomic Analysis of Responses to Drought Stress in Sunflower (Helianthus annuus) Leaves by 2DE Gel Electrophoresis and Mass Spectrometry

Castillejo¹,

Maldonado²,

Ogueta³

et al. 2008

TOPROTJ

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By using a differential proteomic approach, responses to drought stress in sunflower have been studied. Two sunflower genotypes, showing different levels of tolerance to drought have been utilized. Following TCA-acetone protein extraction, the 2-DE leaf protein profile of well watered and drought stressed plants have been compared. Coomassie staining of the gels allowed visualization of around 350 well resolved spots within the 5-8 pH and 10-100 kDa ranges. Image analysis revealed the presence of both, qualitative and quantitative changes between genotypes and treatments. Differential spots were subjected to trypsin digestion and peptides were analyzed by MALDI-TOF mass spectrometry. After database search using peptide mass fingerprinting, 2 genotype-dependent and 23 (susceptible genotype) and 5 (tolerant genotype) stress-responsive protein spots were identified. The two proteins spots differentiating sunflower genotypes corresponded to phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase. In response to drought conditions a general decrease in protein spots corresponding to enzymes of the photosynthesis and carbohydrate metabolism was observed in the more susceptible genotype, suggesting inhibition of the energetic metabolism. Such changes have not been observed in the tolerant genotype, indicating a normal metabolism under drought stress.

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“…The transcriptomic and proteomic approaches developed for this model legume can be used to understand the molecular components and identify candidate genes involved in M. truncatula defence against these pathogens. Transcriptomic or proteomic studies have been performed to determine genes involved in defence mechanisms against Aphanomyces euteiches (Nyamsuren et al 2003;Colditz et al 2005), Erysiphe pisi (Curto et al 2006(Curto et al , 2008Foster-Hartnett et al 2007), Orobanche crenata (Dié et al 2007;Castillejo et al 2009;Dita et al 2009), U. striatus (Castillejo et al 2003) or Mycosphaerella pinodes (Fondevilla et al 2009b).…”

Section: Resultsmentioning

confidence: 99%

Disease resistance in pea (Pisum sativum L.) types for autumn sowings in Mediterranean environments - a review

Rubiales¹,

Fernández‐Aparicio²,

Moral³

et al. 2009

CZECH J GENET PLANT

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Abstract:Pea is an important grain legume mainly grown as spring crop in temperate regions. However, in areas with mild winters and dry springs, like Mediterranean type environments, spring pea types are autumn sown. Unfortunately, little efforts have been made so far in pea breeding for constraints typical of these environments, such as crenate broomrape (Orobanche crenata), rust (Uromyces pisi), powdery mildew (Erysiphe pisi) and ascochyta blight (Mycosphaerella pinodes). In this paper we revise the present state of the art in pea breeding against these diseases and we will critically discuss present progress and future perspectives.

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A proteomic approach to study pea (Pisum sativum) responses to powdery mildew (Erysiphe pisi)

Cited by 95 publications

References 77 publications

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Proteomic Analysis of Responses to Drought Stress in Sunflower (Helianthus annuus) Leaves by 2DE Gel Electrophoresis and Mass Spectrometry

Disease resistance in pea (Pisum sativum L.) types for autumn sowings in Mediterranean environments - a review

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