A proteomic study of pectin‐degrading enzymes secreted by Botrytis cinerea grown in liquid culture

Abstract

Botrytis cinerea is a pathogenic filamentous fungus which infects more than 200 plant species. The enzymes secreted by B. cinerea play an important role in the successful colonization of a host plant. Some of the secreted enzymes are involved in the degradation of pectin, a major component of the plant cell wall. A total of 126 proteins secreted by B. cinerea were identified by growing the fungus on highly or partially esterified pectin, or on sucrose in liquid culture. Sixty-seven common proteins were identified in each of the growth conditions, of which 50 proteins exhibited a Signal P motif. Thirteen B. cinerea proteins with functions related to pectin degradation were identified in both pectin growth conditions, while only four were identified in sucrose. Our results indicate it is unlikely that the activation of B. cinerea from the dormant state to active infection is solely dependent on changes in the degree of esterification of the pectin component of the plant cell wall. Further, these results suggest that future studies of the B. cinerea secretome in infections of ripe and unripe fruits will provide important information that will describe the mechanisms that the fungus employs to access nutrients and decompose tissues.

LongSAGE gene-expression profiling of Botrytis cinerea germination suppressed by resveratrol, the major grapevine phytoalexin

“…We further checked whether these four putative secreted virulence effectors could be found in four recently published secretomes of B. cinerea (Shah et al 2009a(Shah et al , 2009bEspino et al 2010;Fernandez-Acero et al 2010). The putative xyloglucanase (BC1G_00594.1/BofuT4_P021270.1), the putative pectin lyase (BC1G_12017.1/BofuT4_P032630.1) and the putative aspartic protease (BC1G_08393.1/BofuT4_P010710.1) were found in one or several of the published secretomes.…”

Identification of a New Class of Adenosine Deaminase from Helicobacter pylori with Homologs among Diverse Taxa

“…Following denaturing SDS-PAGE of the partially purified sample, five prominent bands from the Coomassie-stained gel were excised and treated separately with trypsin using a variation of a published protocol (16). Gel slices were equilibrated in water for 10 min, followed by incubation in a 1:1 solution of acetonitrile (ACN)-water for three rounds of 15 min each.…”

8 Degradation and Modification of Plant Biomass by Fungi

“…Induction of plant-biomass-related genes in the presence of plant biomass seems to be a universal phenomenon in ascomycete fungi, although significant differences in the specific gene/enzyme sets have been observed. Growth of B. fuckeliana on pectin resulted in the production of 13 pectinrelated enzymes, while only 4 were detected during growth on sucrose (Shah et al 2009), demonstrating that specific induction is the dominant driver of gene expression. Proteomics of extracellular samples from T. reesei grown on corn stover revealed cellulases and xylanases as the major enzymes produced (Nagendran et al 2009), fitting well with this species specialization for these two polysaccharides.…”