LongSAGE gene-expression profiling of Botrytis cinerea germination suppressed by resveratrol, the major grapevine phytoalexin

Zheng, Chuanlin; Choquer, Mathias; Zhang, Bing; Ge, Hui; Hu, Songnian; Ma, Huiqin; Chen, Shangwu

doi:10.1016/j.funbio.2011.06.009

Cited by 12 publications

(18 citation statements)

References 96 publications

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“…With regard to propanoate metabolism, the expression of genes encoding glucan carrier protein is a mitochondrial translocase and promotes the transport of solutes across the mitochondrial membrane; this process between the mitochondrion and cytosol is dependent on ATP [25]. This finding was also contrary to the results reported by Zheng et al (2011). It is noteworthy that qRT-PCR results for Bcin01g00400, Bcin07g06960, Bcin02g03350, and Bcin16g04640 were all consistent with our RNA-seq data (Table S1).…”

Section: Transcriptional Analysis Of Genes Involved In Carbon and Enesupporting

confidence: 79%

“…Protein synthesis, although an exceedingly complicated process, is essential for cellular growth and metabolism. Among the genes associated with protein synthesis in B. cinerea, there were 28 genes enriched in term "ribosome" by KEGG, and their expression was found to be significantly upregulated ( Table S2) previous gene-profiling study on B. cinerea germination reported that transcripts encoding ribosomal proteins were markedly affected upon resveratrol treatment [23].…”

Section: Transcriptional Analysis Of Genes Involved In Amino Acid Metmentioning

confidence: 96%

“…The expression of the 40S ribosomal protein S17 (Bcin12g01300), 60S ribosomal proteins L18ae (Bcin05g01600) and L9 (BC1G_16427), and 60S ribosome biogenesis protein SQT1 (Bcin08g05590) was significantly downregulated, but that of 40S ribosomal proteins S8 (Bcin04g05190) and S5 (Bcin03g00590) and 60S ribosomal proteins L14 (Bcin03g06970), L44 (Bcin09g02490), L9 (Bcin11g05920), and L35 (Bcin11g03280) was significantly upregulated [23]. However, only the trend shown by the 60S ribosomal protein L44 (Bcin09g02490) was the same when B. cinerea was treated with wuyiencin or resveratrol; all other genes were either not differentially expressed or showed contradictory expression trends upon wuyiencin treatment.…”

Section: Transcriptional Analysis Of Genes Involved In Amino Acid Metmentioning

confidence: 99%

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Genome-wide Transcriptomic Analysis of the Response ofBotrytis cinereato Wuyiencin

Shi

Liu

Wei

et al. 2019

Preprint

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AbstractGrey mould is caused by the ascomycetes Botrytis cinerea in a range of crop hosts. As a biological control agent, the nucleoside antibiotic wuyiencin has been industrially produced and widely used as an effective fungicide. To elucidate the effects of wuyiencin on the transcriptional regulation in B. cinerea, we, for the first time, report a genome-wide transcriptomic analysis of B. cinerea treated with wuyiencin. We could identify 2067 differentially expressed genes (DEGs); of them, 886 and 1181 genes were significantly upregulated and downregulated, respectively. Functional categorization indicated that genes involved in amino acid metabolism and those encoding putative secreted proteins were remarkably influenced in response to wuyiencin treatment. Moreover, the expression of genes involved in protein synthesis and energy metabolism (oxidative phosphorylation) and of those encoding ATP-binding cassette transporters was markedly upregulated, whereas that of genes participating in DNA replication, cell cycle, and stress response was downregulated. Furthermore, wuyiencin resulted in mycelial malformation and negatively influenced cell growth rate and conidial yield in B. cinerea. Our results suggest that this nucleoside antibiotic regulates all aspects of cell growth and differentiation in B. cinerea. To summarize, we identified candidate pathways and target genes that may offer insights into the protective and antagonistic mechanisms underlying the action of biological control agents.

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Section: Transcriptional Analysis Of Genes Involved In Carbon and Enesupporting

confidence: 79%

Section: Transcriptional Analysis Of Genes Involved In Amino Acid Metmentioning

confidence: 96%

Section: Transcriptional Analysis Of Genes Involved In Amino Acid Metmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide Transcriptomic Analysis of the Response ofBotrytis cinereato Wuyiencin

Shi

Liu

Wei

et al. 2019

Preprint

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“…Until now, no previous proteomic studies have been reported concerning the intracellular proteins accumulated during germination process in B. cinerea. This process has been studied by transcriptome profiling (Zheng et al 2011;Leroch et al 2013) describing the up-regulation of secreted proteins, different germinationspecific expression pattern and corresponding secondary metabolic pathways. Only a few proteomic approaches concerning the germination process have been reported (Noir et al 2009) describing the first annotated proteome map of ungerminated conidiospores of Blumeria graminis f.sp hordei, the causal agent of powdery mildew in barley, concluding that spores are equipped with the complete molecular machinery to trigger the germination process on a suitable host surface, since most of the identified proteins in these ungerminated conidiospores are involved in carbohydrate, lipid and protein metabolism.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Proteomic profiling of Botrytis cinerea conidial germination

et al. 2014

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Botrytis cinerea is one of the most relevant plant pathogenic fungi. The first step during its infection process is the germination of the conidia. Here, we report on the first proteome analysis during the germination of B. cinerea conidia, where 204 spots showed significant differences in their accumulation between ungerminated and germinated conidia by two-dimensional polyacrylamide gel electrophoresis and qPCR. The identified proteins were grouped by gene ontology revealing that the infective tools are mainly preformed inside the ungerminated conidia allowing a quick fungal development at the early stages of conidial germination. From 118 identified spots, several virulence factors have been identified while proteins, such as mannitol-1-phosphate dehydrogenase, 6,7-dimethyl-8-ribityllumazine synthase or uracil phosphoribosyltransferase, have been disclosed as a new potential virulence factors in botrytis whose role in pathogenicity needs to be studied to gain new insights about the role of these proteins as therapeutic targets and virulence factors.

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“…The role of selected enzymes [8]–[16], metabolites [17]–[21], transporters [22], [23], stress response elements [24], [25], cell wall building enzymes [26], [27] or signalling pathways [28]–[39] was hence revealed or clarified, most times in relation to plant infection. Moreover, the recent release and analysis of the fungus genome sequence [40] has added tremendous force to the task of understanding the necrotrophic plant-fungus interaction, and first outcomes of broader studies have emerged [3], [16], [41]–[43].…”

Section: Introductionmentioning

confidence: 99%

The Homeobox BcHOX8 Gene in Botrytis Cinerea Regulates Vegetative Growth and Morphology

et al. 2012

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Filamentous growth and the capacity at producing conidia are two critical aspects of most fungal life cycles, including that of many plant or animal pathogens. Here, we report on the identification of a homeobox transcription factor encoding gene that plays a role in these two particular aspects of the development of the phytopathogenic fungus Botrytis cinerea. Deletion of the BcHOX8 gene in both the B. cinerea B05-10 and T4 strains causes similar phenotypes, among which a curved, arabesque-like, hyphal growth on hydrophobic surfaces; the mutants were hence named Arabesque. Expression of the BcHOX8 gene is higher in conidia and infection cushions than in developing appressorium or mycelium. In the Arabesque mutants, colony growth rate is reduced and abnormal infection cushions are produced. Asexual reproduction is also affected with abnormal conidiophore being formed, strongly reduced conidia production and dramatic changes in conidial morphology. Finally, the mutation affects the fungus ability to efficiently colonize different host plants. Analysis of the B. cinerea genome shows that BcHOX8 is one member of a nine putative homeobox genes family. Available gene expression data suggest that these genes are functional and sequence comparisons indicate that two of them would be specific to B. cinerea and its close relative Sclerotinia sclerotiorum.

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LongSAGE gene-expression profiling of Botrytis cinerea germination suppressed by resveratrol, the major grapevine phytoalexin

Cited by 12 publications

References 96 publications

Genome-wide Transcriptomic Analysis of the Response ofBotrytis cinereato Wuyiencin

Genome-wide Transcriptomic Analysis of the Response ofBotrytis cinereato Wuyiencin

Proteomic profiling of Botrytis cinerea conidial germination

The Homeobox BcHOX8 Gene in Botrytis Cinerea Regulates Vegetative Growth and Morphology

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