A proteomics approach for the identification of species-specific immunogenic proteins in the Mycobacterium abscessus complex

Steindor, Mathis; Nkwouano, Vanesa; Stefanski, Anja; Stuehler, Kai; Ioerger, Thomas R.; Bogumil, David; Jacobsen, Marc; MacKenzie, Colin R.; Kalscheuer, Rainer

doi:10.1016/j.micinf.2018.10.006

Cited by 12 publications

(15 citation statements)

References 45 publications

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“…S4-S7). The specific sequence for the detection of MABC was described previously 38 . For the detection of MABC, we used a probe for gene encoding uncharacterized protein MAB_0974.…”

Section: Methodsmentioning

confidence: 99%

Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex

Minias

Żukowska

Lach

et al. 2020

Sci Rep

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Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies—M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing.

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“…S4-S7). The specific sequence for the detection of MABC was described previously 38 . For the detection of MABC, we used a probe for gene encoding uncharacterized protein MAB_0974.…”

Section: Methodsmentioning

confidence: 99%

Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex

Minias

Żukowska

Lach

et al. 2020

Sci Rep

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“…Considerable effort would be necessary to optimize and standardize a protocol to obtain reproducible mass spectrometry results for differentiating the M. abscessus complex subspecies. A proteomics study revealed specific immunogenic proteins in the M. abscessus complex; therefore, the subspecies may be differentiated using immunoassays (34). Mutations in rrl gene confer acquired resistance and nucleotide T28 of erm (41) is associated with inducible resistance.…”

Section: Discussionmentioning

confidence: 99%

PCR amplification of the erm(41) gene can be used to predict the sensitivity of Mycobacterium�abscessus complex strains to clarithromycin

et al. 2019

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A worldwide increase in the Mycobacterium abscessus (M. abscessus) complex has been observed. Therefore, the aim of the present study was to investigate the diversity of the rrl and erm(41) genes, both of which are associated with macrolide sensitivity in the M. abscessus complex. The current study also examined the efficacy of mass spectrometry as an alternative to molecular testing to classify subspecies of the M. abscessus complex. A total of 14 strains of the M. abscessus complex were obtained, and based on conventional analyses using housekeeping genes, 57% were determined to be M. abscessus subsp. abscessus, 43% were M. abscessus subsp. massiliense, and none were identified as M. abscessus subsp. bolletii. However, depending on the strain, it was not always possible to distinguish between the subspecies by mass spectrometry. Consequently, PCR products for the rrl and erm(41) genes were directly sequenced. Overall, 7.1% of the strains were identified to have a rrl mutation, and 92.9% carried a T at position 28 of erm (41). Results presented here suggest that the principal cause of treatment failure for M. abscessus complex infections is inducible macrolide resistance encoded by the erm(41) gene. From a strictly pragmatic standpoint, the phenotypic function of a putative erm(41) gene is the most important piece of information required by clinicians in order to prescribe an effective treatment. Although PCR amplification of erm(41) is not sufficient to differentiate between the M. abscessus complex subspecies, PCR can be easily and efficiently used to predict the sensitivity of members of the M. abscessus complex to clarithromycin. PCR amplification of the erm(41) gene can be used to predict the sensitivity of Mycobacterium abscessus complex strains to clarithromycin

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“…massiliense , and these are common in Asia, Oceania and South Korea 11 , 15 , 20 , 21 . These emerging NTMs can cause skin, soft tissue, and central nervous system infections and can also lead to LD 19 , 20 , 22 , 23 . LD caused by MABSC mainly affects patients with predisposed diseases such as cystic fibrosis 20 , 24 .…”

Section: Epidemiologymentioning

confidence: 99%

“…It was later reported that some species were related to medical devices used in surgical procedures or esthetic treatments 25 . Recently, direct transmission of NTM species among cystic fibrosis patients has also been documented 20 .…”

Section: Transmission and Clinical Manifestationsmentioning

confidence: 99%

Non-tuberculous mycobacterial lung disease: a brief review focusing on radiological findings

Anjos

Parreira

Torres

et al. 2020

Rev. Soc. Bras. Med. Trop.

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The incidence and prevalence of lung disease caused by non-tuberculous mycobacteria (NTM-LD) has increased worldwide and its diagnosis represents a complex challenge. This article aims to review the tomographic findings of NTM-LD in order to facilitate their definitive diagnosis. The search for publications on the subject was performed in PMC and Scielo using the keywords 'non-tuberculous mycobacteria', 'lung disease and computed tomography (CT)' and 'radiological findings'. The radiological findings described by 18 articles on mycobacteriosis were reviewed. In addition, CT images of patients diagnosed with NTM-LD were considered to represent radiological findings. Eighteen publications were used whose main findings were pulmonary cavitation (88.9%), bronchiectasis (77.8%), and pulmonary nodules (55.6%). Despite the overlaps in imaging-related analysis of myocobacterioses with other pulmonary infections, such as tuberculosis, the predominant involvement of the middle lobe and lingula should raise suspicion for NTM-LD.

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A proteomics approach for the identification of species-specific immunogenic proteins in the Mycobacterium abscessus complex

Cited by 12 publications

References 45 publications

Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex

Subspecies-specific sequence detection for differentiation of Mycobacterium abscessus complex

PCR amplification of the erm(41) gene can be used to predict the sensitivity of Mycobacterium�abscessus complex strains to clarithromycin

Non-tuberculous mycobacterial lung disease: a brief review focusing on radiological findings

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