A protocol for in vitro mass propagation of asiatic hybrids of lily through liquid stationary culture

Abstract

A simple, rapid and cost-effective in vitro scheme has been proposed for mass propagating two cultivars of Asiatic lily hybrids. An average of seven bulblets was formed after 17 d when 1 Â 1 cm 2 bulb scale segments (explants) were cultured on Murashige and Skoog (MS) medium with 3% sucrose and 0.5 mM a-naphthaleneacetic acid (NAA). On MS medium containing 0.5 mM NAA and 6 or 9% sucrose, depending on the cultivar, large numbers of bulblets of increased size (3.5± 5.0 cm in circumference) were formed under a 16/8 h photoperiod. A continuous system of mass propagation of bulblets was achieved through in vitro scale formation (secondary explants) on MS medium supplemented with 23 mM kinetin and 0.5 mM NAA, as well as scale proliferation on MS basal liquid stationary medium. Upon transplantation all bulblets sprouted, of which 40% flowered in the first season. Under ideal conditions, ca. 9X68 Â 10 5 bulblets can be produced from a single scale segment in 1 yr by following the systematic propagation steps proposed here.

Establishment of genetic fidelity of In vitro-raised Lilium bulblets through rapd markers

“…The standard protocol for raising Lilium (Asiatic hybrids) cultivar`Gran Paradiso' micropropagated bulblets through an adventitious budding method using bulbscale culture was developed by us for large-scale multiplication (Varshney et al, 2000). Bulblets were formed after repeated subculture on semi-solid MS (Murashige and Skoog, 1962) medium supplemented with 0.5 mM 1-naphthaleneacetic acid (NAA) and 6% sucrose every 6 wk.…”

Application of bioreactor systems for large scale production of horticultural and medicinal plants

“…Numerous studies have been reported on regeneration of bulblets from excised lily bulb scales (Robb, 1957;Hackett, 1969;Allen, 1974;Anderson, 1977;Novak and Petru, 1981;Takayama and Misawa, 1983;Varshney et al, 2000) and it is now the current commercial method used for vegetative propagation of lilies. In our previous study, we reported a two-stage culture process of Lilium micropropagation (bulblet formation and then their development) in 5-20 l batches of non-stirred bioreactors within 60 days (Lim et al, 1998;Kim, 1999.…”

Micropropagation: A Tool for the Production of High Quality Plant-based Medicines

“…Extensive work has been done on several ornamental species of family Liliaceae [46,89,102] but very few medicinal plants of this family have been cultured in vitro [39]. Biotechnological research on Chlorophytum borivilianum has been supported previously by central funding agencies.…”