A protocol for Ulmus minor Mill. micropropagation and acclimatization

Conde, Paula; Sousa, Andreia S. P.; Costa, Armando; Santos, Conceição

doi:10.1007/s11240-007-9310-8

Cited by 34 publications

(15 citation statements)

References 21 publications

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“…3). A similar two-step rooting procedure has been used successfully for different species (Martin, 2003;Feyissa et al, 2005;Siddique and Anis, 2007;Husain et al, 2008;Conde et al, 2008). IBA at high concentration is known to suppress root elongation (Korach et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Trejgell¹,

Tretyn²

2011

Acta Biologica Cracoviensia Series Botanica

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This paper is the first published report describing micropropagation of Carlina onopordifolia, using shoot tip and hypocotyl explants. The explants were excised from 10-day-old seedlings and transferred to proliferation medium supplemented with 6-benzylaminopurine (BA; 1.0 or 3.0 mg l -1 ), kinetin (Kn; 1.0 or 3.0 mg l -1 ) or zeatin (ZEA; 1.0 or 3.0 mg l -1 ) in combination with naphthaleneacetic acid (NAA; 0.1 mg l -1 ). The shoot tips were significantly better than hypocotyls as initial material for shoot regeneration. For shoot multiplication, MS medium supplemented with BA proved superior to the other cytokinins tested. Medium supplemented with 1.0 mg l -1 BA gave the highest shoot propagation frequency (66.9%) and number of shoots per explant (2.5). Single shoots were separated from each other and rooted on MS supplemented with IBA for the whole period of culture, with longor short-pulse IBA application. The highest rooting frequency (84.8%) and root number (18.8) were for shortpulse (1 min) 1000 mg l -1 IBA solution. The higher IBA concentration stimulated callus formation and the development of short roots. The shoots were transferred to MS medium without growth regulators. Survival was highest (54.4%) for the plants from the short-pulse 100 mg l -1 IBA treatment. After 8 weeks of acclimatization the plantlets were removed to field conditions and grew normally.K Ke ey y w wo or rd ds s: : Carlina onopordifolia, micropropagation, cytokinins, rooting, auxin.

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Section: Resultsmentioning

confidence: 99%

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Trejgell¹,

Tretyn²

2011

Acta Biologica Cracoviensia Series Botanica

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“…It is anticipated that L. pumila will face extinction and severe genetic loss if necessary cultivation steps are not taken. In this case, plant tissue culture techniques may be able to facilitate the large-scale production of valuable clones to avoid further depletion of this species from its natural habitat (Conde et al, 2008). Significant features of in vitro propagation are the enormous multiplication capacity in a relatively short time span, the production of healthy and disease-free plants, and the ability to generate propagules throughout the year independent of seasonal changes (Christensen and Sriskandarajah, 2008).…”

Section: Introductionmentioning

confidence: 99%

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Ling

Tan

Hussein

2013

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.

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“…Efforts on germplasm preservation and breeding programs involving biotechnological strategies have been encouraged (Harvengt 2004). Research dealing with in vitro micropropagation/acclimatization of U. minor has been successfully conducted (Conde et al 2004, Conde et al 2008, Dias et al 2010. However, up to the knowledge of the authors, information concerning the role of the antioxidant enzymatic system in plant protection against the deleterious effect of ROS formation after ex vitro acclimatization is unknown for this species.…”

Section: Introductionmentioning

confidence: 99%

Acclimatization of micropropagated plantlets induces an antioxidative burst: a case study with Ulmus minor Mill.

Dias¹,

Pinto²,

Santos³

2011

Photosynt.

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In this article, the effects of increased light intensities on antioxidant metabolism during ex vitro establishment of Ulmus minor micropropagated plants are investigated. Three month old in vitro plants were acclimatized to ex vitro conditions in a climate chamber with two different light intensities, 200 µmol m -2 s -1 (high light, HL) and 100 µmol m -2 s -1 (low light, LL) during 40 days. Immediately after ex vitro transfer, the increase of both malondialdehyde (MDA) and electrolyte leakage in persistent leaves is indicative of oxidative stress. As the acclimatization continues, an upregulation of the superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR) enzyme activities were also observed. Simultaneously, MDA content and membrane permeability stabilized, suggesting that the antioxidant enzymes decrease the deleterious effects of reactive oxygen species (ROS) generation. Unexpectedly, newly formed leaves presented a different pattern of antioxidative profile, with high levels of MDA and membrane leakage and low antioxidant enzyme activity. Despite these differences, both leaf types looked healthy (e.g. greenish, with no necrotic spots) during the whole acclimatization period. The results indicate that micropropagated U. minor plantlets develop an antioxidant enzyme system after ex vitro transfer and that, in general, LL treatment leads to lower oxidative stress. Moreover, new leaves tolerate higher levels of ROS without the need to activate the antioxidative pathway, which suggests that the environment at which leaves are exposed during its formation determinate their ability to tolerate ROS.

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A protocol for Ulmus minor Mill. micropropagation and acclimatization

Cited by 34 publications

References 21 publications

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Acclimatization of micropropagated plantlets induces an antioxidative burst: a case study with Ulmus minor Mill.

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