A Purified Agonist-Activated G-Protein Coupled Receptor: Truncated Octopus Acid Metarhodopsin

Ashida, A.; Matsumoto, Katsuya; Ebrey, Thomas G.; Tsuda, Motoyuki

doi:10.2108/zsj.21.245

Cited by 14 publications

(12 citation statements)

References 26 publications

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“…The fractions with this peak were concentrated for 30 min. The SDS-PAGE of the fraction at this peak revealed a purified band to ~33 KDa (Figure 7, below) in complete agreement with the data of [93,94]. Time ago sensors were built either for anesthetic vapor based on bacteriorodhopsin [95] or for routine contaminants testing based on DNA [96] and now our goal is to construct a more effective sensor based on the results so far obtained and those still pending on the Octapus Rodhopsin.…”

Section: Langmuir-blodgett Multilayers Of Proteins For Health and supporting

confidence: 71%

Matrices for Sensors from Inorganic, Organic, and Biological Nanocomposites

Nicolini

Sivozhelezov²,

Bavastrello

et al. 2011

Materials

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Matrices and sensors resulting from inorganic, organic and biological nanocomposites are presented in this overview. The term nanocomposite designates a solid combination of a matrix and of nanodimensional phases differing in properties from the matrix due to dissimilarities in structure and chemistry. The nanoocomposites chosen for a wide variety of health and environment sensors consist of Anodic Porous Allumina and P450scc, Carbon nanotubes and Conductive Polymers, Langmuir Blodgett Films of Lipases, Laccases, Cytochromes and Rhodopsins, Three-dimensional Nanoporous Materials and Nucleic Acid Programmable Protein Arrays.

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Section: Langmuir-blodgett Multilayers Of Proteins For Health and supporting

confidence: 71%

Matrices for Sensors from Inorganic, Organic, and Biological Nanocomposites

Nicolini

Sivozhelezov²,

Bavastrello

et al. 2011

Materials

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“…Although the purification efficiency of the longer melanopsin was lower than the shorter form, it exhibited a similar G protein activation ability (data not shown). In addition, the cephalopod squid and octopus rhodopsins have similar long C‐terminal tails, and enzymatic deletion of the tails does not affect the Gq activation profile (Ashida et al. 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, crystallization of GPCRs and rhodopsins other than bovine rhodopsin has been tried. One of them is on Gq‐coupled rhodopsins, such as octopus and squid rhodopsins (Ashida et al. 2004; Murakami et al.…”

Section: Discussionmentioning

confidence: 99%

Expression and comparative characterization of Gq‐coupled invertebrate visual pigments and melanopsin

Terakita¹,

Tsukamoto²,

Koyanagi³

et al. 2007

Journal of Neurochemistry

105

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A non‐visual pigment melanopsin, which is localized in photosensitive retinal ganglion cells and is involved in the circadian photoentrainment and pupillary responses in mammals, is phylogenetically close to the visual pigments of invertebrates, such as insects and cephalopods. Recent studies suggested that melanopsin is a bistable pigment and drives a Gq‐mediated signal transduction cascade, like the invertebrate visual pigments. Because detailed electrophysiological properties are somewhat different between the visual cells and the photosensitive ganglion cells, we here expressed and purified the invertebrate visual pigment and melanopsin to comparatively investigate their Gq‐activation abilities. We successfully expressed and purified UV and blue light‐sensitive visual pigments of the honeybee as well as the amphioxus melanopsin. Although the purified UV‐sensitive pigment and the melanopsin lost their bistable nature during purification, reconstitution of the pigments in lipid vesicles resulted in return of the bistable nature. The light‐dependent Gq‐activation abilities among these reconstituted pigments are similar, suggesting that the electrophysiological differences do not depend on the Gq‐activation step but rather on the other signal transduction steps and/or on cell properties. Our findings are also important in that this is the first report describes a heterologous large‐scale expression of the Gq‐coupled invertebrate visual pigments in cultured cells.

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“…Tsuda and his colleagues (Ashida et al, 2004) showed that after octopus rhodopsin had a portion of its C-terminal "tail" proteolyzed, the resulting pigment had identical spectral properties compared to the unproteolyzed pigment. Upon irradiation of this species, the resulting mixture of (truncated) rhodopsin and (truncated) Acid Metarhodopsin could be separated on a DEAE column, resulting in a pure and stable preparation of the activating species, Acid Metarhodopsin.…”

Section: Puri>cation Of the Active Form Of An Invertebrate Visual Pigmentioning

confidence: 99%