A putative function of the nucleolus in the assembly or maturation of specialized messenger ribonucleoprotein complexes

Jellbauer, Stephan; Jansen, Ralf‐Peter

doi:10.4161/rna.7163

Cited by 16 publications

(14 citation statements)

References 53 publications

(70 reference statements)

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“…The localization of UPF3 and UPF2 in the nucleolus suggests a model where the NMD proteins are loaded onto the aberrant mRNPs prior to being exported to the cytoplasm where UPF1 (mainly cytoplasmic) is recruited to activate degradation. The assembly of UPF3/UPF2 onto the aberrant transcripts would parallel the model recently proposed for assembly of translationally repressed, localized mRNPs in yeast and animals, and for export-competent viral mRNPs, in the nucleolus before export to the cytoplasm (Hiscox, 2007;Jellbauer and Jansen, 2008).…”

Section: Discussionmentioning

confidence: 94%

“…Localized mRNAs, such as yeast ASH1 mRNA, enter the nucleolus bound to specific RNA binding proteins. In the nucleolus, translation repressor proteins are loaded onto the mRNA before the mRNP is exported to the cytoplasm and its final destination, where translation is activated (Du et al, 2008;Jellbauer and Jansen, 2008). A similar trafficking pathway may also operate in mammals, for the formation of mRNPs associated with Staufen, an RNA binding protein found in the nucleolus and involved in transport of mRNAs in neurons .…”

Section: Discussionmentioning

confidence: 99%

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Aberrant mRNA Transcripts and the Nonsense-Mediated Decay Proteins UPF2 and UPF3 Are Enriched in theArabidopsisNucleolus

Kim¹,

Koroleva

Lewandowska³

et al. 2009

The Plant Cell

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The eukaryotic nucleolus is multifunctional and involved in the metabolism and assembly of many different RNAs and ribonucleoprotein particles as well as in cellular functions, such as cell division and transcriptional silencing in plants. We previously showed that Arabidopsis thaliana exon junction complex proteins associate with the nucleolus, suggesting a role for the nucleolus in mRNA production. Here, we report that the plant nucleolus contains mRNAs, including fully spliced, aberrantly spliced, and single exon gene transcripts. Aberrant mRNAs are much more abundant in nucleolar fractions, while fully spliced products are more abundant in nucleoplasmic fractions. The majority of the aberrant transcripts contain premature termination codons and have characteristics of nonsense-mediated decay (NMD) substrates. A direct link between NMD and the nucleolus is shown by increased levels of the same aberrant transcripts in both the nucleolus and in Up-frameshift (upf) mutants impaired in NMD. In addition, the NMD factors UPF3 and UPF2 localize to the nucleolus, suggesting that the Arabidopsis nucleolus is therefore involved in identifying aberrant mRNAs and NMD.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Aberrant mRNA Transcripts and the Nonsense-Mediated Decay Proteins UPF2 and UPF3 Are Enriched in theArabidopsisNucleolus

Kim¹,

Koroleva

Lewandowska³

et al. 2009

The Plant Cell

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“…In this regard, an association between the nucleolus and export of particular human virus RNAs is well established (Hiscox, 2007;Hiscox et al, 2010) and has been suggested to function for cellular mRNAs. In yeast (Saccharomyces cerevisiae) and animal cells the question of whether specific mRNAs/mRNPs have a nucleolar phase is still open (discussed in Jellbauer and Jansen, 2008).…”

Section: Rna Complexity In the Nucleolusmentioning

confidence: 99%

Nucleoli: Composition, Function, and Dynamics

2011

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“…The nucleolus is a multifunctional nuclear subcompartment whose most prominent and conserved function resides in pre-ribosomal RNA subunits production (for review, see Brown and Shaw 2008). In addition, the nucleolus is a central hub for the biogenesis and TRAMP-mediated quality control of several small noncoding RNPs (Carneiro et al 2007), the correct mRNP assembly of several mRNAs (at least in yeast and mammals) (Du et al 2008;Jellbauer and Jansen 2008) and the recognition and tagging to cytoplasmic nonsense-mediated decay of aberrantly spliced pre-mRNAs (at least in plant) (Kim et al 2009). Whether AtLARP6a may play a role in the noncoding RNA TRAMPmediated surveillance system is not straightforward to predict on the basis of current knowledge.…”

Section: Evolution Of Larp4 and Larp6mentioning

confidence: 99%

The association of a La module with the PABP-interacting motif PAM2 is a recurrent evolutionary process that led to the neofunctionalization of La-related proteins

Merret¹,

Martino²,

Bousquet‐Antonelli³

et al. 2012

RNA

114

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La-related proteins (LARPs) are largely uncharacterized factors, well conserved throughout evolution. Recent reports on the function of human LARP4 and LARP6 suggest that these proteins fulfill key functions in mRNA metabolism and/or translation. We report here a detailed evolutionary history of the LARP4 and 6 families in eukaryotes. Genes coding for LARP4 and 6 were duplicated in the common ancestor of the vertebrate lineage, but one LARP6 gene was subsequently lost in the common ancestor of the eutherian lineage. The LARP6 gene was also independently duplicated several times in the vascular plant lineage. We observed that vertebrate LARP4 and plant LARP6 duplication events were correlated with the acquisition of a PABPinteracting motif 2 (PAM2) and with a significant reorganization of their RNA-binding modules. Using isothermal titration calorimetry (ITC) and immunoprecipitation methods, we show that the two plant PAM2-containing LARP6s (LARP6b and c) can, indeed, interact with the major plant poly(A)-binding protein (PAB2), while the third plant LARP6 (LARP6a) is unable to do so. We also analyzed the RNA-binding properties and the subcellular localizations of the two types of plant LARP6 proteins and found that they display nonredundant characteristics. As a whole, our results support a model in which the acquisition by LARP4 and LARP6 of a PAM2 allowed their targeting to mRNA 39 UTRs and led to their neofunctionalization.

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A putative function of the nucleolus in the assembly or maturation of specialized messenger ribonucleoprotein complexes

Cited by 16 publications

References 53 publications

Aberrant mRNA Transcripts and the Nonsense-Mediated Decay Proteins UPF2 and UPF3 Are Enriched in theArabidopsisNucleolus

Aberrant mRNA Transcripts and the Nonsense-Mediated Decay Proteins UPF2 and UPF3 Are Enriched in theArabidopsisNucleolus

Nucleoli: Composition, Function, and Dynamics

The association of a La module with the PABP-interacting motif PAM2 is a recurrent evolutionary process that led to the neofunctionalization of La-related proteins

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