The aim of this study was to develop “Teachers’ Views on Mixed and Single-gender Education Scale”. The study group consisted of 718 teachers in 18 different schools who volunteered to participate in the study in Küçükçekmece District of İstanbul. Both Exploratory and Confirmatory Factor Analysis were carried out for the validity of the scale. With Exploratory Factor Analysis, it was found that the scale consisted of two factors (positive views on single-gender education and positive views on mixed-gender education) and 18 items and the scale explained 63% of the total variance. When the fit index values of the scale were examined, it was found that the model had a good fit [RMSEA=,05, SRMR=,05, CFI=0.98, GFI=0.93, AGFI=,91, NFI=,95 and IFI=0.98]. In order to calculate the discriminative values of the scale items, mean scores of upper and lower groups were examined with t-test and it was found that all of the items were discriminative. Cronbach Alpha values calculated for the scale reliability were found to vary between, 81 and ,89.