1995
DOI: 10.1111/j.1423-0410.1995.tb01681.x
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A Quantitative Assay for Subclassing IgG Alloantibodies Implicated in Hemolytic Disease of the Newborn

Abstract: Traditionally, IgG subclassing has been performed using qualitative assays. Quantitation of IgG subclasses may have prognostic value in evaluating alloimmunized pregnancies. A quantitative enzyme-linked immunosorbent assay (ELISA) was implemented for measuring IgG subclasses of red blood cell (RBC) antibodies (AB) isolated by adsorption/elution from the sera of alloimmunized pregnant women. The assay is a sandwich enzyme immunoassay using monoclonal antibodies specific for the relevant IgG subclasses and anti-… Show more

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Cited by 14 publications
(5 citation statements)
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“…Since unconjugated bilirubin is absorbed more easily than bilirubin glucuronides, 17 the expected result of ␤-glucuronidase inhibition would be to decrease the intestinal absorption of bilirubin and thus improve bilirubin excretion in the stool. This would fit with the observed order of jaundice in this study: breast milk (rich with ␤-glucuronidase 14,15,21,25 ) is associated with highest jaundice levels, Enfamil (containing negligible ␤-glucuronidase 14 ) is associated with intermediate jaundice levels, and Nutramigen (containing a ␤-glucuronidase inhibitor 20,24 ) is associated with the lowest jaundice levels.…”
Section: Commentsupporting
confidence: 81%
“…Since unconjugated bilirubin is absorbed more easily than bilirubin glucuronides, 17 the expected result of ␤-glucuronidase inhibition would be to decrease the intestinal absorption of bilirubin and thus improve bilirubin excretion in the stool. This would fit with the observed order of jaundice in this study: breast milk (rich with ␤-glucuronidase 14,15,21,25 ) is associated with highest jaundice levels, Enfamil (containing negligible ␤-glucuronidase 14 ) is associated with intermediate jaundice levels, and Nutramigen (containing a ␤-glucuronidase inhibitor 20,24 ) is associated with the lowest jaundice levels.…”
Section: Commentsupporting
confidence: 81%
“…8 It has been suggested that identification of IgG subclasses may yield prognostic information regarding the development of hemolysis and hyperbilirubinemia. 9 Few studies have assessed the role of IgG subclasses in the pathogenesis of hyperbilirubinemia in ABO heterospecificity. On the basis of data retrieved from Rhisoimmunized newborns and 1 study of ABO-heterospecific neonates, 4 we hypothesized that the degree of hemolysis and hyperbilirubinemia would correlate with the anti-A or anti-B IgG subclass present and that the presence of subclass 1 or 3 would be predictive of hemolysis.…”
mentioning
confidence: 99%
“…In such instances, MMA assays appear to have limited predictive value. The IgG subclass quantitation as say described in the current study is not suitable for lab oratories performing routine antenatal tests; however, the ELISA assay described by Thomas et al [22] may be of more practical value for routine laboratories.…”
Section: Discussionmentioning
confidence: 94%