A quantitative framework for the 3D characterization of the osteocyte lacunar system

Mäder, Kevin; Schneider, Philipp; Müller, Ralph; Stampanoni, Marco

doi:10.1016/j.bone.2013.06.026

Cited by 101 publications

(93 citation statements)

References 59 publications

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“…Sharir short morphology, to having long and slender forms while the min-136 eral particle long-axis becomes aligned with the bone long axis. 137 The above mentioned studies reported the morphology and with osteocyte lacunae [25][26][27] in the bone matrix) a 'despeckling' Table 2). By depositing material in both the perios- bone zones $46,000 lacunae/mm 3 are found [5,36].…”

mentioning

confidence: 96%

Long bone maturation is driven by pore closing: A quantitative tomography investigation of structural formation in young C57BL/6 mice

et al. 2015

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mentioning

confidence: 96%

Long bone maturation is driven by pore closing: A quantitative tomography investigation of structural formation in young C57BL/6 mice

et al. 2015

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“…Motion of the sample along three different axes will probably have to be simultaneous and uninterrupted, thus producing a unique three-dimensional scan. With hundreds or thousands of Gigavoxel tomograms collected within a single day, statistically significant ensembles can be surveyed, for instance to study phenotypic variations and drug-dependent effects at the sub-micrometer scale (Mader et al, 2013).…”

Section: Gains In Quantitymentioning

confidence: 99%

Coherent imaging at the diffraction limit

Thibault

Guizar‐Sicairos

Menzel

2014

J Synchrotron Radiat

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X-ray ptychography, a scanning coherent diffractive imaging technique, holds promise for imaging with dose-limited resolution and sensitivity. If the foreseen increase of coherent flux by orders of magnitude can be matched by additional technological and analytical advances, ptychography may approach imaging speeds familiar from full-field methods while retaining its inherently quantitative nature and metrological versatility. Beyond promises of high throughput, spectroscopic applications in three dimensions become feasible, as do measurements of sample dynamics through time-resolved imaging or careful characterization of decoherence effects.

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“…There will always be a compromise between field of view and spatial resolution as well as acquisition time, data volume, and related financial implications. A framework has been proposed for the quantitative characterisation of osteocyte lacunae and their 3D spatial relationships (Mader et al, 2013), which included shape, orientation, number density, spatial distribution, and alignment. This framework is a valuable first step and should be expanded to encompass finer cellular detail such as cell processes and ultrastructure.…”

Section: Pascal Buenzlimentioning

confidence: 99%

High-resolution 3D imaging of osteocytes and computational modelling in mechanobiology: insights on bone development, ageing, health and disease

Goggin

Zygalakis

Oreffo³

et al. 2016

eCM

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Osteocytes are involved in mechanosensation and mechanotransduction in bone and hence, are key to bone adaptation in response to development, ageing and disease. Thus, detailed knowledge of the three-dimensional (3D) structure of the osteocyte network (ON) and the surrounding lacuno-canalicular network (LCN) is essential. Enhanced understanding of the ON&LCN will contribute to a better understanding of bone mechanics on cellular and sub-cellular scales, for instance through improved computational models of bone mechanotransduction. Until now, the location of the ON within the hard bone matrix and the sub-µm dimensions of the ON&LCN have posed significant challenges for 3D imaging. This review identifies relevant microstructural phenotypes of the ON&LCN in health and disease and summarises how light microscopy, electron microscopy and X-ray imaging techniques have been used in studies of osteocyte anatomy, pathology and mechanobiology to date. In this review, we assess the requirements for ON&LCN imaging and examine the state of the art in the fields of imaging and computational modelling as well as recent advances in high-resolution 3D imaging. Suggestions for future investigations using volume electron microscopy are indicated and we present new data on the ON&LCN using serial block-face scanning electron microscopy. A correlative approach using these high-resolution 3D imaging techniques in conjunction with in silico modelling in bone mechanobiology will increase understanding of osteocyte function and, ultimately, lead to improved pathways for diagnosis and treatment of bone diseases such as osteoporosis.Keywords: Osteocyte, 3D imaging, microscopy, b i o m e c h a n i c s , l a c u n o -c a n a l i c u l a r n e t w o r k , mechanobiology, mechanosensation, mechanotransduction, osteoporosis. IntroductionThrough bone adaptation, the skeleton adapts continuously to changed mechanical loading patterns due to development, growth, ageing, disease, disuse or exercise, by removing existing and adding new bone tissue. It has been recognised that osteocytes are the key cells which orchestrate bone adaptation (Tatsumi et al., 2007). Osteocytes are ovoid cells approximately 10 µm long, surrounded by a pericellular matrix (PCM). The osteocytes and their processes form the osteocyte network (ON), which is housed within the lacuno-canalicular network (LCN), a system of voids and channels in the calcified bone matrix (Fig. 1). Neve et al., 2012). Knowledge of the three-dimensional (3D) structure of the ON&LCN would inform conclusions about the function and malfunction of osteocytes for different bone states in development, ageing, disease, disuse or exercise. More specifically, enhanced knowledge would improve the predictive power and accuracy of computational models, which attempt to elucidate the mechanisms of mechanotransduction using 3D geometries for the ON&LCN. Recent finite element and fluid-structure interaction models have used relatively low resolution image data and made a priori assumptions about the ...

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A quantitative framework for the 3D characterization of the osteocyte lacunar system

Cited by 101 publications

References 59 publications

Long bone maturation is driven by pore closing: A quantitative tomography investigation of structural formation in young C57BL/6 mice

Long bone maturation is driven by pore closing: A quantitative tomography investigation of structural formation in young C57BL/6 mice

Coherent imaging at the diffraction limit

High-resolution 3D imaging of osteocytes and computational modelling in mechanobiology: insights on bone development, ageing, health and disease

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