Coherent imaging at the diffraction limit

Thibault, Pierre; Guizar‐Sicairos, Manuel; Menzel, Andreas

doi:10.1107/s1600577514015343

Cited by 61 publications

(58 citation statements)

References 86 publications

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“…In ptychography, coherent scattering patterns are measured from an array of spots in a region of interest (ROI). When the spots are chosen with sufficient overlap, the set of measured elastic scattering patterns can be reduced reliably to the amplitude (modulus) and phase information of the object's transmission, as well as an illumination probe function (25,26). Unlike conventional lens-based microscopic imaging techniques, such as STXM, ptychography is not limited by the properties of the X-ray optics used, has the potential to reach near atomic-scale spatial resolution with very short-wavelength X-rays (27), and can image with Rayleigh wavelength-limited resolution in the soft X-ray region (at the Fe L 3 edge, the wavelength is 1.8 nm).…”

Section: Significancementioning

confidence: 99%

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Measuring spectroscopy and magnetism of extracted and intracellular magnetosomes using soft X-ray ptychography

Zhu

Hitchcock

Bazylinski

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Characterizing the chemistry and magnetism of magnetotactic bacteria (MTB) is an important aspect of understanding the biomineralization mechanism and function of the chains of magnetosomes (Fe 3 O 4 nanoparticles) found in such species. Images and X-ray absorption spectra (XAS) of magnetosomes extracted from, and magnetosomes in, whole Magnetovibrio blakemorei strain MV-1 cells have been recorded using soft X-ray ptychography at the Fe 2p edge. A spatial resolution of 7 nm is demonstrated. Precursor-like and immature magnetosome phases in a whole MV-1 cell were visualized, and their Fe 2p spectra were measured. Based on these results, a model for the pathway of magnetosome biomineralization for MV-1 is proposed. Fe 2p X-ray magnetic circular dichroism (XMCD) spectra have been derived from ptychography image sequences recorded using left and right circular polarization. The shape of the XAS and XMCD signals in the ptychographic absorption spectra of both sample types is identical to the shape and signals measured with conventional bright-field scanning transmission X-ray microscope. A weaker and inverted XMCD signal was observed in the ptychographic phase spectra of the extracted magnetosomes. The XMCD ptychographic phase spectrum of the intracellular magnetosomes differed from the ptychographic phase spectrum of the extracted magnetosomes. These results demonstrate that spectro-ptychography offers a superior means of characterizing the chemical and magnetic properties of MTB at the individual magnetosome level.ptychography | magnetotactic bacteria | biomineralization | STXM |

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Section: Significancementioning

confidence: 99%

“…Ptychography is a coherent diffractive imaging technique (25). In ptychography, coherent scattering patterns are measured from an array of spots in a region of interest (ROI).…”

Section: Significancementioning

confidence: 99%

Measuring spectroscopy and magnetism of extracted and intracellular magnetosomes using soft X-ray ptychography

Zhu

Hitchcock

Bazylinski

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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“…The advent of a fourth-generation source, diffraction-limit storage ring (DLSR), opens a promising pathway for CDI [90][91][92]. The low transverse emittance and beam divergence improve the coherent fraction of the X-ray beam, especially for hard X-rays.…”

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confidence: 99%

Current Status of Single Particle Imaging with X-ray Lasers

Sun

Fan

et al. 2018

Applied Sciences

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Abstract:The advent of ultrafast X-ray free-electron lasers (XFELs) opens the tantalizing possibility of the atomic-resolution imaging of reproducible objects such as viruses, nanoparticles, single molecules, clusters, and perhaps biological cells, achieving a resolution for single particle imaging better than a few tens of nanometers. Improving upon this is a significant challenge which has been the focus of a global single particle imaging (SPI) initiative launched in December 2014 at the Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, USA. A roadmap was outlined, and significant multi-disciplinary effort has since been devoted to work on the technical challenges of SPI such as radiation damage, beam characterization, beamline instrumentation and optics, sample preparation and delivery and algorithm development at multiple institutions involved in the SPI initiative. Currently, the SPI initiative has achieved 3D imaging of rice dwarf virus (RDV) and coliphage PR772 viruses at~10 nm resolution by using soft X-ray FEL pulses at the Atomic Molecular and Optical (AMO) instrument of LCLS. Meanwhile, diffraction patterns with signal above noise up to the corner of the detector with a resolution of~6 Ångström (Å) were also recorded with hard X-rays at the Coherent X-ray Imaging (CXI) instrument, also at LCLS. Achieving atomic resolution is truly a grand challenge and there is still a long way to go in light of recent developments in electron microscopy. However, the potential for studying dynamics at physiological conditions and capturing ultrafast biological, chemical and physical processes represents a tremendous potential application, attracting continued interest in pursuing further method development. In this paper, we give a brief introduction of SPI developments and look ahead to further method development.

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“…Therefore, with the latter approach, we demonstrated ptychographic imaging with a spatial resolution of 10 nm, performed at two detector positions. This all points towards diffraction-limited sources 28 able to provide the most coherent and intense synchrotron X-ray beam. Together with precise in-vacuum scanning X-ray microscopes and future seamless area detectors, they will certainly facilitate robust high-resolution ptychographic imaging.…”

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confidence: 99%

Influence of finite spatial coherence on ptychographic reconstruction

Stachnik

Mohácsi

Vartiainen

et al. 2015

Applied Physics Letters

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X-ray ptychography is an ultrahigh-resolution scanning coherent diffractive imaging technique, allowing quantitative measurements of extended samples and a simultaneous reconstruction of the illuminating wavefront. Recent development of the mixed-state reconstruction algorithm has triggered a certain interest in utilizing partially coherent X-ray sources for ptychography. Here, we study how finite spatial coherence influences the reconstructed image of a test structure. Our work shows that use of a highly coherent illumination provides images with better spatial resolution and fewer artefacts than the approach with partial coherence.

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Coherent imaging at the diffraction limit

Cited by 61 publications

References 86 publications

Measuring spectroscopy and magnetism of extracted and intracellular magnetosomes using soft X-ray ptychography

Measuring spectroscopy and magnetism of extracted and intracellular magnetosomes using soft X-ray ptychography

Current Status of Single Particle Imaging with X-ray Lasers

Influence of finite spatial coherence on ptychographic reconstruction

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