A Quantitative Method of Determining Urinary Phenols

Barness, Lewis A.; Mellman, William J.; Tedesco, Thomas A.; Young, D.G.; Nocho, Robert

doi:10.1093/clinchem/9.5.600

Cited by 26 publications

(3 citation statements)

References 3 publications

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“…Because the determination of molecular weight of herbs is not possible, the determination of phenolic groups in herbal mixtures may provide a means of quality control. The phenolic groups in AFE were determined spectrophotometrically at 700 nm on the basis of a colourimetric reaction with the Folin‐Ciocalteu reagent 13 . Catechin was used as a standard.…”

Section: Methodsmentioning

confidence: 99%

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Antihyperglycaemic And Anti‐Oxidant Properties Of Anoectochilus Formosanus in Diabetic Rats

Shih

Lin

2002

Clin Exp Pharma Physio

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1. In the present study, we investigated aqueous extracts of Anoectochilus formosanus (AFE) for antihyperglycaemic and anti-oxidant effects in diabetic rats induced by streptozotocin (STZ). 2. Diabetic rats were randomly divided into groups and treated orally by gavage with vehicle (distilled water) or AFE (1 and 2 g/kg), once a day for 21 days. 3. At the end of the 21 day period, AFE (2 g/kg) significantly reduced fasting blood glucose, serum fructosamine, triglycerides and total cholesterol compared with vehicle-treated diabetic rats. In vehicle-treated diabetic rats, levels of renal lipid peroxidation were increased, whereas glutathione concentrations were not affected. Renal lipid peroxidation levels were significantly lower and renal reduced glutathione (GSH) concentrations were significantly higher in AFE-treated diabetic rats compared with vehicle-treated diabetic rats. The diabetic kidney in the vehicle-treated group showed a decrease in catalase, but the activity of glutathione peroxidase (GSH-Px) was increased. 4. The activity of catalase, but not GSH-Px, was significantly reversed by AFE treatment. These results indicate that AFE (1 and 2 g/kg) not only possesses an antihyperglycemic effect, but that it may also reduce oxidative stress in diabetic rats.

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Section: Methodsmentioning

confidence: 99%

“…The phenolic groups in AFE were determined spectrophotometrically at 700 nm on the basis of a colourimetric reaction with the Folin-Ciocalteu reagent. 13 Catechin was used as a standard. The concentration of phenolic groups in AFE was 0.21 nmol/g.…”

Section: Extract Preparationmentioning

confidence: 99%

Antihyperglycaemic And Anti‐Oxidant Properties Of Anoectochilus Formosanus in Diabetic Rats

Shih

Lin

2002

Clin Exp Pharma Physio

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“…This is not a normal intermediate of propionate metabolism, but is formed by a side reaction in which methylmalonyl-CoA is deacylated. Methylmalonate excretion has also been observed in vitamin B12-deficient rats (Barness, Flaks, Young, Tedesco & Nocho, 1963; Williams, Spray, Newman & O'Brien, 1969).…”

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confidence: 89%

Gluconeogenesis from propionate in kidney and liver of the vitamin B12-deficient rat

Weidemann

Hems

Williams

et al. 1970

Biochemical Journal

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1. Kidney-cortex slices and the perfused livers of vitamin B(12)-deficient rats removed propionate from the incubation and perfusion media at 33 and 17% respectively of the rates found with tissues from rats receiving either a normal or a vitamin B(12)-supplemented diet. There was a corresponding fall in the rates of glucose synthesis from propionate in both tissues. 2. The addition of hydroxocobalamin or dimethylbenzimidazolylcobamide coenzyme to kidney-cortex slices from vitamin B(12)-deficient rats in vitro failed to restore the normal capacity for propionate metabolism. 3. Although the vitamin B(12)-deficient rat excretes measurable amounts of methylmalonate, no methylmalonate production could be detected (probably because of the low sensitivity of the method) when kidney-cortex slices or livers from deficient rats were incubated or perfused with propionate. 4. The addition of methylmalonate (5mm) to kidney-cortex slices from rats fed on a normal diet inhibited gluconeogenesis from propionate by 25%. 5. Methylmalonate formation is normally only a small fraction of the flux through methylmalonyl-CoA. This fraction increases in vitamin B(12)-deficient tissues (as shown by the urinary excretion of methylmalonate) presumably because the concentration of methylmalonyl-CoA rises as a result of low activity of methylmalonyl-CoA mutase (EC 5.4.99.2). Slow removal of methylmalonyl-CoA might depress propionate uptake owing to the reversibility of the steps leading to methylmalonyl-CoA formation.

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Der Stoffwechsel phenolischer Verbindungen bei Lebererkrankungen mit encephalopathischen Erscheinungen

Ruge

Otto

1969

Verhandlungen Der Deutschen Gesellschaft Für Innere Medizin

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A Quantitative Method of Determining Urinary Phenols

Abstract: A quantitative method for the determination of urinary phenols is described. Data obtained in 20 children by this method are similar to averages in adults obtained by others. This is exceeded by the excretion of a phenylketonuric infant when placed on excessive intakes of phenylalanine.

Cited by 26 publications

References 3 publications

Antihyperglycaemic And Anti‐Oxidant Properties Of Anoectochilus Formosanus in Diabetic Rats

Antihyperglycaemic And Anti‐Oxidant Properties Of Anoectochilus Formosanus in Diabetic Rats

Gluconeogenesis from propionate in kidney and liver of the vitamin B12-deficient rat

Der Stoffwechsel phenolischer Verbindungen bei Lebererkrankungen mit encephalopathischen Erscheinungen

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