A Quantitative Study of Changes in the Human Corpus Luteum Microvasculature during the Menstrual Cycle1

Gaytán, Francisco; Morales, C.; Garcia-Pardo, L.A.; Reymundo, C.; Bellido, C.; Sánchez-Criado, José E.

doi:10.1095/biolreprod60.4.914

Cited by 69 publications

(43 citation statements)

References 22 publications

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“…The presence of a dense capillary network by the mid-luteal phase is required for optimal delivery of progesterone precursors to, and progesterone from, the luteal cells, at a time when progesterone concentrations are at a maximum (Al-zi'abi et al, 2002). These findings are in agreement with reports in other species (Niswender et al, 1979;Gaytan et al, 1999;Dickson and Fraser, 2000). In the present study, a decrease in the endothelial cell proliferation during late regression, and at 36 h after PGF 2␣ administration, coincided with an observed decrease in microvessels and progesterone concentrations (Al-zi'abi et al, 2002).…”

Section: Discussionsupporting

confidence: 94%

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Angiogenesis and vascular endothelial growth factor expression in the equine corpus luteum

Al-zi'abi¹

2003

Reproduction

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Precise pharmacological control of the corpus luteum is important in the manipulation of the oestrous cycle in mares. Angiogenesis plays a key role in the growth and regression of the corpus luteum; therefore, influencing the vasculature of the corpus luteum may offer a novel method for controlling its lifespan. In the present study, changes in angiogenesis and vascular expression of endothelial growth factor (VEGF) were evaluated throughout the luteal phase and after PGF 2␣ -induced luteolysis. Corpora lutea were collected from mares in the early luteal phase (days 3-4), mid-luteal phase (day 10), early regression (day 14), late regression (day 17), and at 12 and 36 h after administration of PGF 2␣ on day 10 of the oestrous cycle. Immunohistochemistry was used to localize Von Willebrand factor and Ki67 in endothelial and proliferating cells, respectively. VEGF mRNA and protein were localized by in situ hybridization and immunohistochemistry. The proliferation index of endothelial cells was intense in the early luteal phase. The early and mid-luteal phases were characterized by a dense network of capillaries. The microvasculature started to regress by day 14. After administration of PGF 2␣ , vasodilation was observed after 12 h, but after 36 h, luteal degeneration was accompanied by a significant decrease in vascularity. VEGF mRNA and protein were expressed mainly in the luteal cells during the early and mid-luteal phases and expression declined at early regression (day 14). However, immunostaining for VEGF protein was high in late luteal regression (day 17) and 36 h after PGF 2␣ administration. These findings indicate a close temporal association between VEGF expression and angiogenesis in the equine corpus luteum during its functional lifespan.

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Section: Discussionsupporting

confidence: 94%

“…on luteal cells and the remainder of the luteal tissue ×100% (Ferrara et al, 1998;Gaytan et al, 1999). The area of vWF immunostaining was calculated using the Quantimet Image Processing and Analysis System 500 (Leica, Cambridge).…”

Section: Quantification Methodsmentioning

confidence: 99%

Angiogenesis and vascular endothelial growth factor expression in the equine corpus luteum

Al-zi'abi¹

2003

Reproduction

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“…In the present study, the high number of blood vessels in the late CL, associated with a decrease in vascular areas, might be explained by a decrease in blood vessel lumen and by vessel contraction giving the appearance of concentric rings [36]. This decrease in capillary diameter may lead to a fall in blood flow and could initiate and/or accelerate CL regression [38]. The further fall in blood vessel density and number observed in the mare's CA, might be ascribed to the occlusion of blood vessels that disappear as the residual luteal structure organizes into the corpus albicans [36].…”

Section: Discussionmentioning

confidence: 75%

Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle

Ferreira‐Dias¹,

Pinto‐Bravo²,

Mateus³

et al. 2006

Domestic Animal Endocrinology

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Corpus luteum growth and endocrine function are closely dependent on the formation of new capillaries. The objectives of this study were to evaluate (i) tissue growth and microvascular development in the equine cyclic luteal structures; (ii) in vitro angiogenic activity of luteal tissues in response to luteotrophic (LH, PGE 2 ) and luteolytic (PGF 2␣ ) hormones and (iii) to relate data to luteal endocrinological function. Our results show that microvascular density was increased in the early and mid luteal phase, followed by a fall in the late luteal phase and a further decrease in the corpus albicans. Hyperplasia of luteal tissue increased until the mid luteal phase and it was followed by tissue regression. Luteal explants were cultured with no hormone added, or with PGF 2␣ , LH, PGE 2 , LH + PGE 2 or LH + PGF 2␣ . Media conditioned by equine luteal tissue from different stages of the luteal phase were able to stimulate mitogenesis of bovine aortic endothelial cells (BAEC), suggesting the presence of angiogenic activity. No difference was observed among luteal structures on their mitogenic capacity, for any treatment used. Nevertheless, Late-CL conditioned-media with PGF 2␣ showed a significant decrease in BAEC proliferation (p < 0.05) and LH + PGF 2␣ a tendency to reduce mitogenesis. Thus, prostaglandin F 2␣ may play a role on vascular regression of the CL during the late luteal phase in the * Corresponding author. Tel.: +351 213652859; fax: +351 213652889. -mail address: gmlfdias@fmv.utl.pt (G. Ferreira-Dias Ferreira-Dias et al. / Domestic Animal Endocrinology 30 (2006) [247][248][249][250][251][252][253][254][255][256][257][258][259] mare. These data suggest that luteal angiogenesis and vascular regression in the mare are coordinated with the development of non-vascular tissue and might be regulated by many different factors. E

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“…In the ovaries of SAMR mice, strong expressions of eNOS at estrus stage (C lane 2) and of iNOS at estrus and metestrus stages (E lanes 2 and 3) were detected. f u n ct i on o f th e l u te a l c el l s, p ro g es t er o n e p r o d uc t i o n , i s a c c o m p a n ie d b y e x te n s i v e development of the vascular system in LBs [21] and by maximal dilatation of LB capillary vessels [22]. It has been reported that dilatation of the vascular bed in LBs of rat ovaries is mediated by NO, and that the expression of eNOS mRNA in luteal cells, indicating NO production, is related to a decrease in progesterone synthesis [22].…”

Section: Discussionmentioning

confidence: 98%

Abnormal Accumulation of Luteal Bodies in Ovaries of the Senescence Accelerated Mouse (SAM).

Kiso

Manabe

Komatsu

et al. 2001

Journal of Reproduction and Development

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Abstract. The senescence accelerated mouse prone (SAMP) mice with a shortened life span show accelerated changes in many of the signs of aging and have a shorter reproductive life span than SAM resistant (SAMR) controls. The reproductive senescence of SAMP is more accelerated than that of SAMR. In the present study, we found abnormal accumulation of luteal bodies (LBs) only in the ovaries of SAMP mice and, we examined the mechanism of abnormalities in luteal cell regression in the ovaries of SAMP mice. No significant changes were detected in serum progesterone or 17β-estradiol levels during estrus cycle between SAMP and SAMR mice. In abnormally accumulated LBs of SAMP mice, extremely high levels of activity of 20α-hydroxysteroid dehydrogenase, which catalyzes conversion of progesterone to the inactive form, 20α-hydroxyprogesterone were demonstrated histochemically. However, no marked differences in activity of 17β-hydroxysteroid dehydrogenase were detected histochemically. Moreover, no apoptotic cells were detected in the abnormally accumulated LBs of SAMP mice. Extremely weak immunohistochemical reactivity for endothelial nitric oxide synthase (eNOS) was noted in the abnormally accumulated LBs of SAMP mice, but no differences were seen in inducible NOS (iNOS). No marked differences in expression of eNOS-or iNOS-mRNA were seen during the estrus cycle in the ovaries of SAMP mice. However, high levels of expression of eNOS-mRNA at estrus stage and of iNOS-mRNA at estrus and metestrus stages were noted in the ovaries of SAMR mice. SAMP mice may be a useful model in which to examine the mechanism of regression of LBs in mammalian ovaries.

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A Quantitative Study of Changes in the Human Corpus Luteum Microvasculature during the Menstrual Cycle1

Cited by 69 publications

References 22 publications

Angiogenesis and vascular endothelial growth factor expression in the equine corpus luteum

Angiogenesis and vascular endothelial growth factor expression in the equine corpus luteum

Microvascularization and angiogenic activity of equine corpora lutea throughout the estrous cycle

Abnormal Accumulation of Luteal Bodies in Ovaries of the Senescence Accelerated Mouse (SAM).

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