A Rabbit Model of Spontaneous Thrombosis Induced by Lipopolysaccharide

Liu, Haixia; Hou, Jingbo; Hu, Sining; Du, Xiao‐Jun; Yan, Fuhua; Jia, Haibo; Feng, Lifang; Zhang, Linna; Du, Jing; Zhao, Qi; Xie, Zulong; Yu, Bo

doi:10.5551/jat.22772

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…Other information, including demographic variables, level of education, smoking status and personal medical history, was collected in 2002 and 2007 [20]. Level of education was divided into two categories according to whether the participants received a college education (education time ≤ 12 or > 12 years).…”

Section: Methodsmentioning

confidence: 99%

The association between Helicobacter pylori seropositivity and risk of new-onset diabetes: a prospective cohort study

Zhou

Liu

et al. 2017

Diabetologia

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Aims/hypothesis Previous studies have suggested a possible connection between Helicobacter pylori (H. pylori) infection and diabetes risk. However, prospective studies examining direct associations between these two factors are relatively lacking. In this prospective cohort study, we aimed to evaluate the association between H. pylori infection and risk of developing diabetes. Methods We performed a population-based prospective study, recruiting participants aged 45-74 years and without diabetes from the Chinese Multi-provincial Cohort Study in 2002, with a 10 year follow-up to investigate development of diabetes. H. pylori serostatus was determined by measuring serum H. pylori antibodies. H. pylori seropositivity was defined as the antibody concentration ≥ 10 U/ml. To examine the association between H. pylori seropositivity and diabetes risk, modified Poisson regression was performed. Results Of 2085 participants without diabetes, 1208 (57.9%) were H. pylori seropositive in 2002. After multivariate adjustment of possible diabetes risk factors, H. pylori seropositivity was associated with lower risk of diabetes (RR 0.78 [95% CI 0.63, 0.97], p = 0.022). Of the 1275 participants with H. pylori antibody measurements in both 2002 and 2007, 677 (53.1%) were persistently seropositive. A lower risk of diabetes was also observed in participants with persistent H. pylori seropositivity (RR 0.61 [95% CI 0.41, 0.93], p = 0.020), compared with those persistently seronegative. Conclusions/interpretation H. pylori seropositivity was associated with lower risk of diabetes in this prospective cohort study. Extrapolation of these results and the mechanism underlying the observed association require further investigation.

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Section: Methodsmentioning

confidence: 99%

The association between Helicobacter pylori seropositivity and risk of new-onset diabetes: a prospective cohort study

Zhou

Liu

et al. 2017

Diabetologia

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“…As shown in Table 1, the main species used to study thrombosis are zebrafish [25][26][27], mice [8,[28][29][30][31][32][33], rats [34,35], rabbit [36][37][38] hamsters [39,40], guinea pigs [41,42], pigs [43][44][45], dogs [46][47][48], and baboons [9,49,50]. Of course, the mouse remains the reference animal model in the study of cardiovascular disease for several reasons: (1) it is a mammalian species with many physiological similarities to humans, (2) it is economical to house, (3) it is easy to manipulate, and (4) its genome is easily modifiable, allowing the creation of numerous transgenic lines [25].…”

Section: Animal Modelsmentioning

confidence: 99%

Role of Neutrophils and NETs in Animal Models of Thrombosis

Carminita

Crescence

Panicot‐Dubois

et al. 2022

IJMS

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Thrombosis is one of the major causes of mortality worldwide. Notably, it is not only implicated in cardiovascular diseases, such as myocardial infarction (MI), stroke, and pulmonary embolism (PE), but also in cancers. Understanding the cellular and molecular mechanisms involved in platelet thrombus formation is a major challenge for scientists today. For this purpose, new imaging technologies (such as confocal intravital microscopy, electron microscopy, holotomography, etc.) coupled with animal models of thrombosis (mouse, rat, rabbit, etc.) allow a better overview of this complex physiopathological process. Each of the cellular components is known to participate, including the subendothelial matrix, the endothelium, platelets, circulating cells, and, notably, neutrophils. Initially known as immune cells, neutrophils have been considered to be part of the landscape of thrombosis for more than a decade. They participate in this biological process through their expression of tissue factor (TF) and protein disulfide isomerase (PDI). Moreover, highly activated neutrophils are described as being able to release their DNA and thus form chromatin networks known as “neutrophil extracellular traps” (NETs). Initially, described as “dead sacrifices for a good cause” that prevent the dissemination of bacteria in the body, NETs have also been studied in several human pathologies, such as cardiovascular and respiratory diseases. Many articles suggest that they are involved in platelet thrombus formation and the activation of the coagulation cascade. This review presents the models of thrombosis in which neutrophils and NETs are involved and describes their mechanisms of action. We have even highlighted the medical diagnostic advances related to this research.

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A previously unreported impact of a PLA2G7 gene polymorphism on the plasma levels of lipoprotein-associated phospholipase A2 activity and mass

Zhao

Jia³

et al. 2016

Sci Rep

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Lipoprotein-associated phospholipase A2 (Lp-PLA2) levels are associated with the development of atherosclerosis. We aimed to assess the genetic determinants of Lp-PLA2 activity and mass by genotyping multiple polymorphisms in PLA2G7, the gene encoding Lp-PLA2, among 1258 participants from the Chinese Multi-provincial Cohort Study-Beijing Project. The Sequenom MassARRAY system, Taqman assay and direct sequencing were adopted. For the first time, the rs13218408 polymorphism was found to be significantly associated with reduced Lp-PLA2 levels. We also confirmed the significant association of previously validated polymorphisms (rs1421378, rs1805018, rs16874954 and rs2216465), even after adjusting for traditional cardiovascular risk factors and for Bonferroni correction. Percentages of variance attributable to rs13218408 were 7.2% for activity and 13.3% for mass, and were secondary to those of rs16874954 (8.1% for activity and 16.9% for mass). A significant joint effect of rs13218408 and rs16874954 was observed on Lp-PLA2 activity (P = 0.058) and mass (P = 0.003), with their minor alleles together linking to the largest reduction in Lp-PLA2 levels (37.8% reduction in activity and 41.6% reduction in mass). Taken together, our findings show a significant association of a PLA2G7 polymorphism with Lp-PLA2 levels, which was previously unreported in any population. The functionality of this genetic variation deserves further investigations.

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A Rabbit Model of Spontaneous Thrombosis Induced by Lipopolysaccharide

Cited by 4 publications

References 31 publications

The association between Helicobacter pylori seropositivity and risk of new-onset diabetes: a prospective cohort study

The association between Helicobacter pylori seropositivity and risk of new-onset diabetes: a prospective cohort study

Role of Neutrophils and NETs in Animal Models of Thrombosis

A previously unreported impact of a PLA2G7 gene polymorphism on the plasma levels of lipoprotein-associated phospholipase A2 activity and mass

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