A radioimmunoassay for alpha-melanotropin in rat plasma

Wilson, John F.; Morgan, Merrill A.

doi:10.1016/0160-5402(79)90034-2

Cited by 34 publications

(16 citation statements)

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“…Cross-reaction with 1-24 corticotrophin (Synacthen; Ciba-Geigy) was 002% that of a-melanotrophin; ß-bovine-melanotrophin (Ciba-Geigy) had a similarly weak crossreaction and produced a non-parallel displacement curve. The detection limit for the combined assay and extraction procedures was from 3 to 10 pmol -melanotrophin/l for plasma samples (Wilson & Morgan, 1979a) and 0004 pmol a-melanotrophin/pituitary gland from the present pituitary assays. The coefficient of variation of the logarithm of the plasma a-melanotrophin measurements was estimated, from the observed differences between replicated plasma measurements, as 9-5% (74 degrees of freedom).…”

Section: Animalsmentioning

confidence: 99%

“…Plasma samples were extracted before assay by adsorption to Florisil (Sigma Chemical Co., London; Wilson & Morgan, 1979a). The recovery in the 13 assays in which the present samples were included was 56 + 5% (mean ±s.e.m.).…”

Section: Animalsmentioning

confidence: 99%

“…Pituitary samples were homogenized in 0-5 ml glacial acetic acid and the extracts, collected after centrifugation, dried in vacuo at room temperature. The residues were resuspended in 0-5 ml assay buffer (Wilson & Morgan, 1979a) and each sample was tested in duplicate at four concentrations in the assay. The concentrations tested were doubling dilutions from either full strength or a 1 in 100 dilution of the pituitary extracts.…”

Section: Animalsmentioning

confidence: 99%

“…Assay of a-melanotrophin The concentration of a-melanotrophin in the pituitary gland and plasma samples was determined by radioimmunoassay (Wilson & Morgan, 1979a). Plasma samples were extracted before assay by adsorption to Florisil (Sigma Chemical Co., London; Wilson & Morgan, 1979a).…”

Section: Animalsmentioning

confidence: 99%

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LEVELS OF Α-Melanotrophin IN THE PLASMA AND PITUITARY GLANDS OF FETAL AND JUVENILE RATS, AND IN THE PLASMA OF ADULT FEMALE RATS DURING PREGNANCY AND LACTATION

Wilson¹,

Morgan²

1980

Journal of Endocrinology

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alpha-Melanotrophin was detected by radioimmunoassay in the pituitary glands of fetal rats from day 17 of gestation. The pituitary content of alpha-melanotrophin increased regularly, at a gradually decreasing rate, throughout gestation and in the postnatal period. Concentrations of alpha-melanotrophin in the plasma of fetal and newborn rats were below the detection limit of the radioimmunoassay (10 pmol/l). Detectable concentrations were first found in young rats on day 3 after birth and did not differ significantly from those in their mothers throughout the period of suckling. Plasma concentrations of alpha-melanotrophin were raised in pregnant rats during the last 4 days of gestation and after parturition. They returned to basal levels in the 2 weeks after delivery. After weaning at 3 weeks of age, a large increase in the plasma concentration of alpha-melanotrophin was detected in juvenile rats. Plasma levels had returned to the normal adult range by 6 weeks of age. The increases in alpha-melanotrophin in the blood were thought to be the result of non-specific stress effects. The data did not provide evidence for a role of alpha-melanotrophin in reproductive processes in the rat.

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Section: Animalsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

See 2 more Smart Citations

LEVELS OF Α-Melanotrophin IN THE PLASMA AND PITUITARY GLANDS OF FETAL AND JUVENILE RATS, AND IN THE PLASMA OF ADULT FEMALE RATS DURING PREGNANCY AND LACTATION

Wilson¹,

Morgan²

1980

Journal of Endocrinology

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“…Extracts were applied to: (i) Sephadex G-25 SF or G-50 SF columns (10 X 1000 mm), eluted with Anolis buffer, 0.25 M ammonium bicarbonate, pH 8.2, or 0.1 M acetic acid containing 0.5% bovine serum albumin; (ii) Amberlite XAD-2 columns, precycled, washed, and eluted as described (22) (4); (ii) a specific a-MSH assay, employing antiserum Albert (final dilution, 1:16,000) and synthetic a-MSH, iodinated by the chloramine-T method (24) and purified by Quso glass absorption (24), and chromatography on Sephadex G-25 SF, having a specific activity of 450-50 Ci/,ug (1 Ci = 3.7 X 1010 becquerels). Samples were assayed in dilutions in duplicates and sample volume (150 1,u) was 10% ofincubation volume.…”

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confidence: 99%

Adrenocorticotropin-like and alpha-melanotropin-like peptides in a subpopulation of human gastrin cell granules: bioassay, immunoassay, and immunocytochemical evidence.

Larsson¹

1981

Proc. Natl. Acad. Sci. U.S.A.

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Adrenocorticotropin (ACTH)-like and a-melanotropin (a-MSH)-like peptides have been localized to a subpopulation of cytoplasmic (secretory) granules of human antropyloric gastrin cells and of fetal and neoplastic gastrin cells. These granules also store gastrin and belong to the electron-dense variety of gastrin cell granules. Gastrin Immunocytochemistry. Sections pretreated as described (3,11), were processed for indirect immunocytochemistry, using a-MSH antisera Albert, Asger, Axel, and Arnold raised in rabbits against synthetic a-MSH (Peninsula Laboratories, San Carlos, CA), coupled to bovine serum albumin by carbodiimide. Optimal dilutions (from 1:500 to 1:10,000) were identical in pituitary intermediate lobes and antropyloric mucosa. In addition, NH2-terminal-specific ACTH-(1-24) antisera (no. 94, Ab. Sigurth) and antisera recognizing the COOH-terminal (14-17) (nos. 4562 and 2717) and middle (6-13) regions (no. 4710) of synthetic human gastrin-17 were used (3,14,15 2990The publication costs ofthis article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

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Low permeability of the blood-brain barrier to nanomolar concentrations of immunoreactive alpha-melanotropin

Wilson

1988

Psychopharmacology

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The permeability of the blood-brain barrier (BBB) to immunoreactive alpha-melanotropin (alpha-MSH) was quantified in rats pretreated with monosodium L-glutamic acid to deplete their CNS stores of endogenous alpha-MSH. The methodology, suitable for poorly permeable substances, monitored blood and brain tissue concentrations of alpha-MSH over 15 min following intravenous injection of 30 nmol synthetic alpha-MSH. Rate constants for entry of alpha-MSH into brain tissue were estimated from separate non-linear least-squares fits of connecting two- and one-compartment open models to plasma and extravascular brain tissue concentration data, respectively. Comparisons were made to rate constants measured similarly for 14C-inulin. The BBB had a low permeability to immunoreactive alpha-MSH, consistent with peptide penetrating the barrier by passive diffusion dependent upon the lipid solubility of the molecule.

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A radioimmunoassay for alpha-melanotropin in rat plasma

Cited by 34 publications

References 34 publications

LEVELS OF Α-Melanotrophin IN THE PLASMA AND PITUITARY GLANDS OF FETAL AND JUVENILE RATS, AND IN THE PLASMA OF ADULT FEMALE RATS DURING PREGNANCY AND LACTATION

LEVELS OF Α-Melanotrophin IN THE PLASMA AND PITUITARY GLANDS OF FETAL AND JUVENILE RATS, AND IN THE PLASMA OF ADULT FEMALE RATS DURING PREGNANCY AND LACTATION

Adrenocorticotropin-like and alpha-melanotropin-like peptides in a subpopulation of human gastrin cell granules: bioassay, immunoassay, and immunocytochemical evidence.

Low permeability of the blood-brain barrier to nanomolar concentrations of immunoreactive alpha-melanotropin

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