A Radioimmunoassay for Plasma Aldosterone<sup>1</sup>

Mayes, Darrel M.; Furuyama, Shunsuke; Kem, David C.; Nugent, Charles A.

doi:10.1210/jcem-30-5-682

Cited by 338 publications

(79 citation statements)

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“…Plasma aldosterone was measured by radioimmunoassay as described elsewhere. 15 Results are expressed as means ± SEM: Analysis of variance was used for among-group comparisons and for comparison of dose-response curves. The Scheffe test was used for specific between-group comparisons.…”

Section: Methodsmentioning

confidence: 99%

Cyclosporin A-induced hyperreninemic hypoaldosteronism. A model of adrenal resistance to angiotensin II.

Stern¹,

Lustig²,

Petrasek³

et al. 1987

Hypertension

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SUMMARY We studied the effects of cyclosporin A on the renin-aldosterone axis in SpragueDawley rats. Two weeks of intragastric administration of cyclosporin A (5 mg/kg/day or 20 mg/kg/day) resulted in large increases in plasma renin concentration (23 ± 5, 70 ±12, and 79 ± 11 ng/ml/hr in control rats and rats receiving 5 mg and 20 mg of cyclosporin A, respectively), with no parallel increments in plasma aldosterone. In vitro angiotensin II (ANG II)-stimulated aldosterone secretion by zona glomerulosa cells obtained from cyclosporin A-treated rats was also reduced (4.8 ± 0.5, 1.5 ± 0.2, and 0.2 ± 0.2 ng/10 5 cells in control rats and rats receiving 5 mg and 20 mg of cyclosporin A, respectively). In contrast, in vitro aldosterone response to graded increments of potassium (3.7-10.7 mmol/L) or adrenocorticotropic hormone (ACTH) (10~"-10~8 M) was preserved in cyclosporin A-treated rats. When added in vitro to zona glomerulosa cells from untreated rats, cyclosporin A also attenuated ANG Il-stimulated aldosterone secretion, but did not affect potassium or ACTH-mediated aldosterone production. Thus, cyclosporin A-induced hyperreninemic hypoaldosteronism in the rat depends on opposing renal and adrenal effects, with a direct or feedback stimulation of renin secretion and a specific blockade of ANG H-mediated aldosterone production. has been occasionally associated with hyperkalemia, hyperchloremic acidosis, relative hypoaldosteronism, and reduced urinary excretion of potassium.1 " 4 The inappropriately normal aldosterone levels in hyperkalemic CSA-treated humans have been interpreted to reflect the coexisting relative hyporeninemia commonly observed in such patients. 1 In the rat, however, CSA administration appears to stimulate plasma renin activity (PRA).5~7 Yet in a recent study, hyperkalemia with relative hypoaldosteronism was observed in CSA-treated rats fed a high potassium diet.

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Section: Methodsmentioning

confidence: 99%

Cyclosporin A-induced hyperreninemic hypoaldosteronism. A model of adrenal resistance to angiotensin II.

Stern¹,

Lustig²,

Petrasek³

et al. 1987

Hypertension

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“…6 Plasma concentrations of aldosterone, growth hormone and insulin were determined by the radioimmunoassay methods of Mayers, et al, 7 Schalch and Parker, s and Moran and Lazarow, 9 respectively. The recovery rates were 85 percent for aldosterone, 98per cent for growth hormone and 94 per cent for insulin.…”

Section: Methodsmentioning

confidence: 99%

Effect of anaesthesia and surgery on endocrine function in elderly patients

Oyama

Taniguchi

Takazawa

et al. 1980

Canad. Anaesth. Soc. J.

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The effect of halothane-nitrous oxide anaesthesia and abdominal surgery" on endocrine function was investigated in 18 elderly patients whose ages ranged from 65 to 77 (mean 69 years).Cortisol levels in plasma increased significantly during anaesthesia and surgery, and the increases were slightly higher than those in young adults. Plasma aldosterone increased and insulin levers did not change during the procedures, and these levels were lower than in younger adults.The endocrine responses in elderly patients were similar in magnitude to those in young adults. Therefore it appears that no special hormonal treatment is necessary for elderly patients undergoing routine surgical procedures.MANY STUDIES ARE AVAILABLE on the effect of anaesthesia and surgery on the endocrine function in adults. ~-s Although the number of elderly patients submitted to operation has increased, no information is available on endocrine responses in these patients. The present investigation was undertaken to determine whether or not hormonal responses in the elderly are different from those in younger adults, because the hormonal function might play a role in the perioperative and postoperative metabolic responses of surgical patients. METHODSEighteen patients of either sex ranging in age from 65 to 77 years, (mean 69 + 0.9 years) served as subjects of this study. ASA classifications were 1 or 2, and none of the patients had any abnormality of endocrine, hepatic or renal function. All of them underwent abdominal operations. Premedication consisted of pentobarbitone 50 mg, meperidine 35 mg and atropine 0.5 mg given 1.5 hours before the start of anaesthesia. Anaesthesia was induced with halothane, nitrous oxide and oxygen, and the operation was not started until 20 minutes after induction.The trachea was intuhated after the first blood sample had been obtained and following intravenous injection of succinylcholine chloride 40rag. Thereafter anaesthesia was maintained with halothane (0.5-1.0 per cent), nitrous oxide

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“…These methods have been previously described for this laboratory (Balment, Henderson & Oliver, 1975;Balment, Chester Jones, Henderson & Oliver, 1976a). Aldosterone and corticosterone in adrenal venous and peripheral arterial blood plasmas were assayed by radioimmunoassays (Mayes, Furayama, Kem & Nugent, 1970;Gross, Ruder, Brown & Lipsett, 1972 Plasma composition was largely unaffected in either group of rats on the Li supplement; the only significant change was the mild hypokalaemia of the LE rats (P < 0.001) after 12 days. The plasma Li concentrations achieved were similar in the two types of rat.…”

Section: Analytical Methods and Hormone Assaysmentioning

confidence: 99%

Time Course of Lithium‐induced Alterations in Renal and Endocrine Function in Normal and Brattleboro Rats With Hypothalamic Diabetes Insipidus

Balment

Jones

Henderson

1977

British J Pharmacology

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I A lithium chloride (1.1 g/kg) supplemented diet was given to Long Evans (LE) and Brattleboro (DI) rats to investigate its actions in the presence (LE) and absence (DI) of vasopressin. 2 During the first 24 h, Li-supplemented LE rats displayed an initial water deficit (drinking less than renal output), increased plasma antidiuretic (ADH) titres and slightly increased plasma renin activities (PRA) and plasma osmolarities. Such changes were qualitatively similar to those seen in rats fed a normal diet, but deprived of water for 24 hours. After 12 days, the Li-supplemented rats had elevated plasma ADH titres, but reduced pituitary oxytocic and antidiuretic activities. 3 The urinary losses of Na, K and Cl exceeded dietary intakes in LE rats on the introduction of the Li-supplement, and the urinary osmolarity fell by 50%. Electrolyte balances were gradually reestablished, although drinking and urine production increased in parallel to reach twice the control values by day 12 of the supplement. 4 Aldosterone and corticosterone secretory rates and their peripheral plasma concentrations were unchanged both after 24 h and 28 days of the Li-supplement. 5 Li elicited no water deficit or saluresis in DI rats, and although the polyuria and polydipsia were exacerbated, urinary osmolarity did not change over the 12 day observation period. 6 Li increased Ca excretion in both rat types; after 12 days the PRA of DI but not LE animals was increased. 7 It is concluded that the overall renal actions of Li are tempered by vasopressin rather than adrenocorticosteroids.

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A Radioimmunoassay for Plasma Aldosterone¹

Cited by 338 publications

References 0 publications

Cyclosporin A-induced hyperreninemic hypoaldosteronism. A model of adrenal resistance to angiotensin II.

Cyclosporin A-induced hyperreninemic hypoaldosteronism. A model of adrenal resistance to angiotensin II.

Effect of anaesthesia and surgery on endocrine function in elderly patients

Time Course of Lithium‐induced Alterations in Renal and Endocrine Function in Normal and Brattleboro Rats With Hypothalamic Diabetes Insipidus

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A Radioimmunoassay for Plasma Aldosterone1

Cited by 338 publications

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Cyclosporin A-induced hyperreninemic hypoaldosteronism. A model of adrenal resistance to angiotensin II.

Cyclosporin A-induced hyperreninemic hypoaldosteronism. A model of adrenal resistance to angiotensin II.

Effect of anaesthesia and surgery on endocrine function in elderly patients

Time Course of Lithium‐induced Alterations in Renal and Endocrine Function in Normal and Brattleboro Rats With Hypothalamic Diabetes Insipidus

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A Radioimmunoassay for Plasma Aldosterone¹